Fig. 2 | Nature Communications

Fig. 2

From: Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont

Fig. 2

Agarolytic reaction pathway of Bu NP1 agarases. a Quantification of GH16B, GH16C, and GH86 activity using agarose, raw porphyran, and enriched porphyran. Error (s.d.) is represented by error bars over three replicates. b TLC analysis of the exo-activity of GH117s and GH2s. FACE analysis of agarose digestion with c GH86-containing and d GH16B-containing reaction mixtures. FACE analysis of NAOS digestion with e GH86-containing and f GH16B-containing reaction mixtures. g Schematic of the agarolytic reaction pathway. The β-1,4 linkages of agarose and large NAOS are hydrolyzed by GH86 and GH16B to release smaller NAOS fragments. Next, the newly exposed α-1,3-linked AHG is cleaved from the non-reducing end by GH117B to generate AOS products, which are substrates for GH2C. GH2C hydrolyzes the β-1,4-linked galactose at the non-reducing end to regenerate NAOS with a degree of polymerization that has collectively been reduced by a single disaccharide. Depolymerization is cyclical and is repeated by alternating reaction steps with GH117B and GH2C

Back to article page