Fig. 9 | Nature Communications

Fig. 9

From: A systematic approach to the development of a safe live attenuated Zika vaccine

Fig. 9

Decreased vertical transmission during DN-2 infection of pregnant A129 mice. a DN-1, DN-2 and H/PF/2013 were tested in a pregnant mouse model according to the timeline shown. be The dams (n = 5 for DN-1 and DN-2, n = 4 for H/PF/2013) were killed at day 7 post infection. Of these mice (infected dams numbered M1 to M14 with n = number of fetuses indicated, uninfected controls numbered C1–C6), four each in the DN-1 and DN-2-infected dams and three in the H/PF/13-infected group were pregnant and the placenta and fetuses were also collected. b Fetuses of infected pregnant dams showing absence or presence of gross pathological changes caused by vertical ZIKV transmission. c Survival and d weight of fetuses were determined and e viral RNA quantification was performed on whole fetus homogenates using qPCR. See also Supplementary Fig. 6. Error bars represent s.d. Dotted lines on viral quantification graphs represent limit of detection