Fig. 8 | Nature Communications

Fig. 8

From: A systematic approach to the development of a safe live attenuated Zika vaccine

Fig. 8

DN-2 demonstrates decreased organ infection. a Frequency of viral RNA detection and b levels of viral RNA detected in the brains, testes, kidneys, and spleens using at 15 days post challenge or at killing for animal study in Fig. 5. c Sequence of viral RNA detected in testes samples were determined using Sanger sequencing to distinguish identity of virus. d Male mice (n = 3 per group) were infected with 104 p.f.u. of DN-1, DN-2 and H/PF/2013 i.p., killed at 8 days post infection and viral loads in the brains, testes, kidneys, and spleens were quantified using qPCR with normalization against viremia. Error bars represent s.d. *p < 0.05, **p < 0.01 in unpaired t test. Dotted lines on viral quantification graphs represent limit of detection or when y-value = 0 in normalized values. n.s. not significant, n.d. not detectable