Fig. 7 | Nature Communications

Fig. 7

From: A systematic approach to the development of a safe live attenuated Zika vaccine

Fig. 7

DN-2 displays attenuated replication in A129 mouse model and elicits protective responses against lethal wild-type ZIKV challenge. Male A129 mice were inoculated i.p. with 103 or 104 p.f.u. of DN-1, DN-2, or H/PF/2013 (n = 5 per group except n = 4 for mice that received 103 p.f.u. of DN-1 and H/PF/2013) and monitored for a survival, b weight changes and viremia levels after infection with c 103 and d 104 p.f.u. of each virus. e Immune sera from surviving mice obtained at 21 days post inoculation to determine neutralizing antibody titers using PRNT and the log 50% neutralizing titers [log(PRNT50)] are as shown. These mice, together with a group of mice that received PBS at day 0 (n = 5), were challenged with 104 p.f.u. of H/PF/2013 i.p. at 21 days post inoculation. f Survival, g weight changes, and h viremia levels were tracked for 15 days or until euthanasia for control mice with >20% weight loss. Error bars represent s.d. *p < 0.05, **p < 0.01 in unpaired t test. Dotted lines on viral quantification graphs represent limit of detection. n.s. not significant, n.d. not detectable