Fig. 4 | Nature Communications

Fig. 4

From: A systematic approach to the development of a safe live attenuated Zika vaccine

Fig. 4

Efficient replication and immune gene activation in monocyte-derived dendritic cells (moDCs). Viral replication in moDCs at 24 h post infection as determined by a qPCR (n = 3, representative plot of two experiments) and b plaque assay (n = 6) on Vero (results of two experiments combined, YF17D plaque assay was performed on BHK-21). c, d Microarray was performed on infected moDCs (n = 3) after 24 h post infection and analysis was done in comparison to uninfected cells (n = 3). c GSEA analysis of the most enriched signaling pathways in DN-2 compared to DN-1, H/PF/2013, and YF17D after 24 h post infection in moDCs. Normalized enrichment scores (NES) were computed by GSEA (n = 3). d Heat map of differentially expressed immune-related genes. Values represented as log2(fold change) compared to uninfected cells. Error bars represent s.d. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 in unpaired t test. n.s. not significant