Fig. 2 | Nature Communications

Fig. 2

From: A systematic approach to the development of a safe live attenuated Zika vaccine

Fig. 2

Genome stability of DN-1 and DN-2 after serial passage. Whole-genome sequencing of DN-1 and DN-2 at each of seven serial passages was performed using next-generation sequencing to determine stability of the M66V mutation and identify additional mutations, if any, after each serial passage. Single-nucleotide variants (SNVs), with >0.01 (1%) frequency, at different genome positions at each serial passage are plotted to determine genome stability. Each data point represents the frequency at a serial passage. Box and whiskers plots show the range of frequencies of SNVs detected over the serial passages, with the box representing the 25th–75th percentiles while the whiskers represent the maximum and minimum frequencies detected. Dotted line at the SNV frequency of 0.5 represents the point above which a variant is recognized as the consensus sequence