Fig. 4 | Nature Communications

Fig. 4

From: Molecular demultiplexer as a terminator automaton

Fig. 4

T-1 signals apoptosis by switching to diagnostic mode. a PE-Annexin labels most of the cells incubated with T-1 under light irradiation (Annexin V (+) region of the green area). b Green channel: cells incubated with T-1 under irradiation. c Green channel: cells incubated with T-1 in the dark. d The 2D plot for both green and red channels: T-1 and PE-Annexin V (a specific apoptosis marker) stain the same kind of cells with a large (86%) agreement: 70% of the irradiated cells were co-stained with both T-1 and PE-Annexin V, indicating only apoptotic cells are fluorescently labeled with T-1. e The truth table with input, the switch and the outputs clearly identified, and the particular set of conditions were highlighted. f Switch input (appearance of phosphatidylserine in the external leaflet of the cell membrane) selects fluorescence emission as the primary output. g Cells treated with PE-Annexin V and the T-1, and kept in dark, show no signs of morphological change, and the cellular membranes are not stained with either one of the agents. h, i Cells were incubated with T-1, irradiated with the LED light source, then treated with PE-Annexin V. The two agents (T-1 and PE-Annexin V, green and red, respectively) label the same regions in the cells undergoing apoptosis. (scale bar, 10 μm)

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