Fig. 6 | Nature Communications

Fig. 6

From: Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation

Fig. 6

Electrostatic interactions that drive conformational compaction allosterically couple the R-motif binding and rRNA binding modes of NPM1. Phase separation diagrams based on turbidity assays for the homotypic (a), heterotypic with SURF6-N (NPM1 constructs at 20 μM) (b) and heterotypic with rRNA (c) mechanisms, at the indicated NPM1 construct (a, c) or SURF6-N (b) concentrations. The purple dotted line is a visual aid that represents the phase boundary for NPM1WT between the mixed (gray circles) and demixed (green circles) states

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