Fig. 2 | Nature Communications

Fig. 2

From: Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation

Fig. 2

Intra-IDR interactions drive structural compaction within NPM1 and influence the threshold for heterotypic LLPS in the presence of SURF6-N. Phase diagrams determined by turbidity for NPM1WT (a), NPM1N240 (b), and NPM1N188 (c). The dotted purple line is a visual aid that represents the phase boundary for NPM1 between the mixed (gray circles) and demixed (green circles) states. (d) Changes in radii of gyration as a function of ionic strength, as determined from P(r) analysis of SAXS scattering curves; fitting errors are shown. (e) smFRET histograms showing the variation of NPM1 conformation at increasing [NaCl]. The solid lines represent fitting of the experimental data with a Gaussian model. The peak at zero is due to molecules lacking an active acceptor dye. The dotted line indicates the shot-noise simulation at each condition

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