Fig. 1 | Nature Communications

Fig. 1

From: Self-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation

Fig. 1

Electrostatic interactions drive NPM1:SURF6-N LLPS. a Net charge per residue distribution using CIDER (http://pappulab.wustl.edu/CIDER/analysis/); top: the central intrinsically disordered region (IDR) in NPM1 exhibits polyampholityc properties; IDR contains two strong acidic tracts (A2 & A3) interleaved with moderately charged basic tracts (B1 & B2); bottom: the intrinsically disordered nucleolar protein SURF6 contains multivalent R-motifs throughout its primary structure. Data are shown for residues 1–182, corresponding to the construct used in this study (SURF6-N); b Schematic representation of the NPM1 constructs used in this study; c Confocal microscopy images of phase separation by 20 µM NPM1WT with 20 µM SURF6-N in buffer with variable concentrations of NaCl, as indicated. Screening of electrostatic interactions at high [NaCl] disrupts LLPS droplets. NPM1 is labeled with AlexaFluor 488 and SURF6-N with AlexaFluor 647. Scale bar, 10 µm

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