Fig. 6: | Nature Communications

Fig. 6

From: A regulatory circuit of two lncRNAs and a master regulator directs cell fate in yeast

Fig. 6

Distribution of IME1 expression among single S288C cells in the presence or absence of IRT2 transcription. a Distribution of IME1 transcripts levels among single cells during entry into meiosis in S288C as detected by single-molecule RNA fluorescence in situ hybridization. Wild-type (WT) (FW631), pCUP-IRT2 (FW2668), and pIME1-Δu6 (FW1390) cells were grown in rich medium till saturation and subsequently shifted to SPO. Samples were taken at the indicated time points, fixed, and hybridized with probes directed against IME1 and ACT1 mRNAs (see supplemental information for details). The black line indicates the median number of transcripts per cell for each time point. At least n = 120 cells were used for the analysis. b Same as a, but cells were binned according IME1 expression levels. c Quantification of cells that completed meiotic divisions in S288C cells described in a. Cells were fixed after 72 h in SPO, stained, and DAPI masses were counted. The means ± SEM of at least n = 5 experiments are shown. ***p < 0.0005 (Student’s t test)