Fig. 4 | Nature Communications

Fig. 4

From: Precise control of SCRaMbLE in synthetic haploid and diploid yeast

Fig. 4

Sporulation and deep sequencing analysis. a The diploid yJBD001 was sporulated and dissected on permissive agar plates, and the resulting haploid spore clones were analyzed to identify the mating type. b The crossing-over of two larger chromosome fragments was observed in the haploid spore yJBS001. The synIII YCR018C-YCR098C segment (168 kb) was exchanged with the wtIII YCR018C-YCR098C, and the YER032W-YER139C of synV (225 kb) was exchanged with the YER032W-YER139C of V. c Three inversions were observed in the haploid spore yJBS001, including one inversion in synV (YEL059W-YEL022W) and two inversions (YCR007C and YCR008W) in synIII. A translocation of YER043C-YER044C was observed in synV. d The copy numbers (duplication and deletion) of five locations in the diploid yeast yJBD001 and its spore yJBS001 were verified by qPCR. Error bars represent SD from three independent experiments

Back to article page