Influenza A virus nucleoprotein (NP) association with viral RNA (vRNA) is essential for packaging, but the pattern of NP binding to vRNA is unclear. Here we applied photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation (PAR-CLIP) to assess the native-state of NP–vRNA interactions in infected human cells. NP binds short fragments of RNA (~12 nucleotides) non-uniformly and without apparent sequence specificity. Moreover, NP binding is reduced at specific locations within the viral genome, including regions previously identified as required for viral genome segment packaging. Synonymous mutations designed to alter the predicted RNA structures in these low-NP-binding regions impact genome packaging and result in virus attenuation, whereas control mutations or mutagenesis of NP-bound regions have no effect. Finally, we demonstrate that the sequence conservation of low-NP-binding regions is required in multiple genome segments for propagation of diverse mammalian and avian IAV in host cells.
Influenza A virus (IAV) possesses a segmented, negative-sense RNA genome that is bound by the viral nucleoprotein (NP) throughout replication. Recent cryo-electron microscopy studies of the IAV ribonucleoprotein complex (vRNP) provide evidence for a NP–RNA complex structure with a corkscrew-like morphology and the tripartite polymerase complex at one end and a loop at the other end. However, current models conflict with each other and yield no information about RNA conformation, binding, or NP–RNA association1,2. NP is thought to coat viral RNA (vRNA) uniformly in cells and virus particles; however. uniform coating likely would preclude the possibility for RNA structure formation in RNPs. Numerous virus families utilize structured RNA elements for specific biological processes throughout infection, including genome packaging3,4. For example, viral RNA elements are required for efficient replication, mRNA splicing, and genome packaging of IAV5,6,7. Structure formation has been demonstrated with in vitro folded IAV vRNA and engineered genome segments, but the structural constraints imposed by nucleoprotein on vRNA generated during infection is not known8,9. Elucidation of the physiological interaction between NP and viral genomic RNA may provide novel insights into how IAV is capable of coordinating its lifecycle. Thus, we set out to determine the in vivo landscape of NP–vRNA interactions.
Infection and complete replication of IAV requires delivery of all eight genome segments into a recipient cell. All IAV segments require packaging signals derived from the termini on each segment10,11. Interaction between vRNAs has been demonstrated in vitro and disruption of packaging signals or interacting segment regions attenuated virus replication at the stage of genome packaging12,13,14. In many cases, mutation of a single segment leads to a significant decrease in the packaging efficiency of other segments5,15. Additionally, viral particles largely package only one copy of each genome segment16,17,18. Together, these results suggest that genome segments function as a multipartite, cooperatively packaged entity, possibly potentiated by segment–segment interactions, rather than a stochastically generated particle19,20.
In this study, we set out to determine how IAV NP interacts with vRNA during infection in cells. We show that the NP of IAV binds the vRNA non-uniformly and that regions of low-NP binding are enriched for predicted RNA secondary structures. Synonymous mutations designed to destabilize the predicted RNA structure attenuate IAV replication, whereas synonymous mutations that maintain the predicted RNA structure or mutations in NP-bound RNA regions have no effect on virus replication in vitro or in vivo. Viral attenuation is associated with an increase in defective virus production, suggesting that low-NP-binding regions and the predicted RNA structures are required for viral genome packaging.
Nucleotide resolution mapping of NP–vRNA interactions
Photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation (PAR-CLIP) coupled to next-generation sequencing was used to resolve the interaction between the negative-sense RNA genome of IAV and NP during infection of human 293T cells21. We infected human cells with WT-PR8 virus for 16 h in the presence of 4-thiouridine (4-SU) to enhance cross-linking of NP–RNA complexes and then generated Illumina 1 × 50 sequencing libraries of the NP-bound RNA (Fig. 1a). The impact of 4-SU on viral replication was assessed in 293T cells. WT-PR8 replicated to equivalent titers 12, 18, and 24 h post-infection (hpi) in mock- or 4-SU-treated (100 μM) cells (Fig. 1b). Additionally, NP localization after 4-SU treatment was assessed by confocal microscopy at 16 hpi, and no alteration was observed at this time point (Fig. 1b). These results suggest that 4-SU treatment does not substantially impact IAV nucleoprotein production or replication in human cells.
To determine the sensitivity and specificity of the PAR-CLIP assay, we performed Western blotting analysis for IAV NP and cellular ß-actin on input lysate and immunoprecipitated proteins (Fig. 1c, bottom). Compared to immunoprecipitations performed without antibody or a control anti-HA antibody, immunoprecipitation with a monoclonal antibody (MAb) against IAV NP produced a specific band. UV-exposure of infected cells in the presence of 4-SU enabled greater recovery of NP–RNA complexes (Fig. 1c, top). The protein purity in the immunoprecipitate was verified by silver stain or Western blotting with an anti-IAV polyclonal serum (Supplementary Fig. 1a, b).
Influenza A nucleoprotein binds viral RNA non-uniformly
PAR-CLIP identified both human and virus-derived RNAs that interacted with NP. The procedure enriched for IAV RNA sequences relative to RNA-seq libraries (Fig. 1d), and the majority of the viral RNA sequences were derived from the negative-strand vRNA (Supplementary Fig. 2). The average length of the NP-bound vRNA was 12 nucleotides (range 11–14, Fig. 1e).
We then compared PAR-CLIP and RNA-seq libraries to identify contiguous regions of vRNA that are significantly under- and overrepresented among the NP-bound RNAs. Using the criteria of >3-fold difference, Q < 0.01, and ≥ 18 nucleotides long, we identified 24 regions in the viral genome that were low in NP binding relative to RNA-seq and 18 regions that met two of these three criteria that we did not investigate further (Supplementary Fig. 3, Supplementary Table 1). Moreover, four high-NP-binding regions where vRNA was overrepresented in PAR-CLIP libraries relative to the control RNA-seq sets were identified (Supplementary Table 1). The low-NP-binding regions together represent ~10% of the viral genome and do not differ in base composition from the remainder of the genome (Fig. 1f). Analysis of the nucleotide sequences in low-NP-binding regions revealed that RNA secondary structures are predicted to form in the absence of NP binding in some of these regions (Supplementary Table 1)5,7. Thus, NP binding might be affected by local secondary structures in the genome of IAV.
Segment 5 low-NP-binding regions are important for IAV
To assess the significance of the NP-bound or underrepresented vRNA regions, we selected six regions in segment 5 that have variable NP-binding profiles (Fig. 2a and Table 1). The lack of alternative reading frames and splicing of segment 5 mRNA made this segment highly suitable for extensive characterization of the significance of the low-NP-binding regions on IAV replication. Of the six regions, two were underrepresented (low-NP binding) in PAR-CLIP data sets (NP22–68 and NP1410–1495). The NP1410–1495 region contains a previously hypothesized vRNA pseudoknot5. Additional regions of NP intermediate binding (NP145–175, NP456–490, NP584–608, and NP1058–1081) were also included in the following studies. Computational prediction of RNA structures using RNAfold in low-NP-binding regions guided further mutational analysis, and these regions were mutagenized to either disrupt (NP22–68:A and NP1410–1495) or maintain (NP22–68:B) the predicted secondary and tertiary structure formation (Supplementary Fig. 4). Mutant viruses bearing 2–7 synonymous structural nucleotide changes in these regions were generated and assessed for ability to replicate in vitro and in vivo.
WT and mutant viruses were subjected to a focus-forming assay in MDCK cells to determine replication competence in vitro (Fig. 2b). Mutations disrupting the segment 5 vRNA pseudoknot (NP1410-1495) formation and destabilizing a predicted stem-loop structure (NP22–68:A) in the 3′ region of the vRNA segment resulted in reduction of focus area, as a measure of multi-cycle replication and spread (Fig. 2b). Conversely, mutation of intermediate NP-bound vRNA regions did not alter the focus area. Synonymous mutations in NP22–68 designed to maintain the predicted secondary structure (NP22–68:B), also did not affect focus size (Fig. 2b). Multi-cycle replication assays in MDCK cells of select mutant viruses confirmed these results (Fig. 2c). Finally, mice were inoculated with 103 TCID50 of each virus, and the presence of infectious virus in the lung was assessed 48 h later. WT-PR8 replicated to high titers at this time point whereas destabilizing mutations in low-NP-binding regions resulted in attenuation (Fig. 2d). Synonymous structural mutations in NP-bound regions had no effect on virus infection in vivo (Fig. 2d). These results collectively suggest that structural features of the low-NP-binding regions are important for IAV replication.
To determine the cause of attenuated replication of low-NP-binding mutant viruses, we evaluated effects on specific stages in the IAV lifecycle. All viruses displayed similar cytoplasmic distribution of NP 8 hpi in MDCK cells when assessed by indirect immunofluorescence and confocal microscopy (Fig. 3a). The ability of the NP proteins from mutant viruses to facilitate the transcription and replication of a firefly luciferase reporter genome segment by the tripartite IAV polymerase complex was tested in human cells; all viruses displayed equivalent reporter activity (Fig. 3b). Similarly, infection of MDCK cells with all viruses generated equivalent amounts of viral antigen (NP) 8 hpi when measured by flow cytometry (Fig. 3c). Therefore, the synonymous mutations introduced within NP segment did not alter NP expression or its activity by all measurable outcomes.
We next assessed the virus particle to infectious unit ratio of WT and mutant PR8 viruses to evaluate potential defects in viral genome packaging. The infectious titer (TCID50 ml−1) of NP1410–1495 and NP22–68:A mutant viruses was significantly lower (P < 0.05) compared to all other viruses when normalized to HA-units, indicating a greater production of non-infectious particles (Fig. 3d). The ability of WT and mutant viruses to package all eight genome segments was assessed using a population level measure of relative vRNA segment abundance in purified viral particles22. RNA from WT and mutant IAVs was subjected to RT-qPCR and the abundance of segments was normalized to segment 7 (Fig. 3e). Mutation of either the predicted pseudoknot (NP1410–1495) or stem-loop structure (NP22–68:A) resulted in aberrant genome constellation stoichiometry. Consistent with the viral replication assays, mutation of intermediate NP-bound regions or mutations designed to maintain the predicted 3′ stem-loop structure (NP22–68:B) result in unaltered ratios of genome segments in all but one segment-specific instance (Fig. 3e and Supplementary Fig. 5a). These results suggest that RNA structure-destabilizing mutations, in regions of vRNA poorly bound by NP, impact replication at the stage of coordinated genome packaging. Finally, we determined the co-expression of NP and matrix (M) proteins in singly infected cells (Fig. 3f, multiplicity of infection (MOI) of 0.05)23. In agreement with the RT-qPCR data, suggesting a packaging defect, a lower percentage of NP1410–1495 and NP22–68:A mutant virus-infected cells co-expressed both proteins compared to cells infected with WT or other mutant viruses. Notably, high MOI infection with multiple defective particles simultaneously infecting the same cell is able to overcome this defect (Supplementary Fig. 5b).
Additional low-NP-binding regions impact IAV replication
Low-NP-binding regions in segments 1, 2, and 8 also were evaluated for their contribution to viral replication by mutagenesis designed to disrupt predicted secondary and tertiary RNA structures (Fig. 4a–c, Supplementary Table 1 and Supplementary Data 1). Nucleotide substitutions that alter the predicted RNA secondary structure but have no impact on the protein, i.e., synonymous structural mutations, in segment 1 (PB21823–1944:A-C), segment 2 (PB1497-561:A) and segment 8 (NS23–86:A) reduced the focus area of mutant viruses in MDCK cells (Fig. 4d–f). Control mutations, designed to maintain the predicted vRNA structure in the low-NP-binding regions on segment 2 and 8 (PB1497–561:B, or NS23–86:B), did not alter focus size (Fig. 4e, f). Similar to the attenuation of structurally disrupted segment 5 viruses, the PB21823–1944:A-B, PB1497–561:A or NS23–86:A mutant viruses generated fewer TCID50 particles per HA-unit, while the control mutations (PB1497–561:B or NS23–86:B) did not (Fig. 4g–i). The PB21823–1944 mutant that showed the least attenuation in the focus-forming assay, PB21823–1944:C, had no discernable difference in TCID50 per HA-unit. Segment 1 (PB2) and 2 (PB1) mutants did not display altered reporter activity (Supplementary Fig. 6a). In contrast to the impact of synonymous mutations in low-NP-binding regions, mutations in NP-bound regions in segment 1 (PB2350–375 and PB22213–2239) and segment 2 (PB11828–1858 and PB12032–2058) did not alter focus size or TCID50 per HA-unit (Fig. 4d, e, g, h, and Supplementary Fig. 5a). Specific manipulation of segment 8 vRNA sequence (NS23–86:A) resulted in decreased packaging of segments 3, 4, 5, and 7 relative to segment 1 (Fig. 4j). Assessment of vRNA abundance in virus particles revealed that segment 2 (PB1497–561:A) mutant viruses packaged reduced levels of segment 6 vRNA (NA) (Fig. 4j). The PB21823–1944:A virus did not show altered segment abundance (Fig. 4k and Supplementary Fig. 6b). Finally, virus replication of the PB21823–1944:A, PB1497–561:A and NS23–86:A mutant viruses in mice was diminished 48 hpi compared to WT-PR8 (Fig. 4k). Together, these data suggest that vRNA sequences that are low in NP binding help to coordinate packaging of a full complement of eight vRNA segments and changes to the predicted RNA structures in these regions results in virus attenuation. To demonstrate that synonymous structural mutations affect the stability of the predicted RNA structure, we performed RNA thermal stability assays using RNA oligomers corresponding to the low-NP-binding region PB1497–56124. The thermal profile of the RNA containing the predicted structure-destabilizing mutations (PB1497–561:A) was different from that of the WT RNA oligomer and the RNA containing synonymous mutations that maintained the RNA structure (PB1497–561:B) (Supplementary Fig. 4d). Moreover, the thermal melt profile for the destabilizing mutations (PB1497–561:A) indicated a lower thermal stability. In contrast, the thermal profile of the PB1497–561:B RNA was similar to that of WT RNA. Combined these data show that stable RNA structures are required for IAV replication and genome packaging.
Mutation of low-NP-binding regions attenuates diverse IAV
We evaluated the contribution of two low-NP-binding regions (PB21823–1944 and NP1410–1495), identified in WT-PR8, to the replication of a North American avian IAV (A/shorebird/Delaware/22/2006 (IAV-H7N3)) and the 2009 pandemic H1N1 IAV (A/California/04/2009 (IAV-pH1N1)). The PB2 and NP gene-segment of IAV-PR8 and IAV-H7N3 are divergent and representative of mammalian and avian viruses, respectively (Fig. 5a, b, Supplementary Fig. 4). The NP gene-segment of IAV-pH1N1 is closely related to IAV-PR8 (Fig. 5a, Supplementary Fig. 4), while the PB2 gene-segment is more closely related to avian viruses (Fig. 5b, Supplementary Fig. 4). Isogenic IAV-H7N3 and IAV-pH1N1 viruses, bearing synonymous mutations in the NP1410–1495 or PB21823–1944 regions, designed to disrupt the predicted local RNA structure, were evaluated for virus replication in vitro. Synonymous mutations designed to impact the predicted RNA secondary structure in these regions of IAV-H7N3 and IAV-pH1N1 attenuated viral replication, as measured by focus area (Fig. 5c, e). All mutant viruses displayed decreased TCID50 per HA-unit (Fig. 5d, f). These results indicate that the RNA features, identified by PAR-CLIP in IAV-PR8, are important in genome packaging of diverse avian and human strains of IAV.
We have identified the interaction landscape of IAV NP with viral RNA in the context of infected cells. Our findings indicate that binding of NP to viral RNA is restricted to an average of 12 nucleotides and the distance between two cross-linking sites is 25 nucleotides. These estimates agree with molecular models of NP–RNA interactions and the NP-binding footprint of a related orthomyxovirus1,20,25,26. Within this model, NP is excluded from consistent interaction with specific regions of vRNA and allows trans-interactions with either other genome segment vRNA or host and virus factors. The interaction between NP and the viral RNA was non-uniform and characterized by regions that were consistently low or high in NP binding. These results are in agreement with recent findings from Lee et al.27. About half of the low-NP regions are predicted to form secondary and tertiary RNA structures, based on computational analysis, and mutations designed to alter the stability of these predicted RNA structures resulted in attenuated virus infection (summarized in Table 1). The presence of RNA structures in RNP complexes may explain the pleomorphic nature of RNPs28. Several, mostly shorter low-NP-binding regions were not predicted to form stable structures. These regions may represent a portion of a larger but less stable structure or have a different yet unknown function during IAV replication. At present, the function of high-NP-binding vRNA regions is unknown. These regions may have a role in inter-segment NP-based interactions or be regions of high-NP density, enabled by position along the vRNP.
We examined NP–vRNA interactions at a late time point when a majority of viral RNA is distributed throughout the cytoplasm and thought to be within vRNP complexes19,29. In support of this, the great majority of PAR-CLIP reads were obtained from negative-sense vRNAs.
We found many low-NP-binding RNA regions that overlap with previously predicted packaging and bundling signals near segment termini (NP22–68, NP1410–1495, and NS23–88)5,30. However, additional low-NP-binding regions were identified throughout the segment body and outside of traditional packaging signals11,22,31. Our findings agree with studies assessing structure-guided mutagenesis of hypothetical vRNA structures (segment 7) or biochemical analyses of vRNA (segment 8) that largely overlap with low-NP-binding regions assessed here7,32. Additionally, we identified specific nucleotides required for co-packaging of segment 2 (PB1) and segment 6 (NA), in a region recently implicated in directing reassortment outcomes and co-segregation of these segments in vitro33,34. Therefore, NP PAR-CLIP allowed us to directly identify potentially functional RNA regions in IAV genomes required for coordinated genome packaging.
As predicted RNA structures in some low-NP-binding regions are important for genome packaging, it suggests a requirement for inter-segment RNA interactions. Specific RNA interactions between genome segments have been demonstrated in vitro and ablation of these interactions lead to aberrant genome packaging; an outcome reversed by introduction of compensatory mutations in the interacting segment12. Many of the mutations that alter genome assembly also lead to an increase in number of defective viral particles30. The formation and nature of vRNA structures is likely contingent on the sequence composition and physical position of the nucleotides within a RNP complex. Additionally, manipulation of NP-bound regions, which may be unable to make inter-segment contacts due to NP-induced structural constraints on vRNA, did not impact virus replication. The presence of highly NP-bound vRNA regions may represent genomic regions that are spatially or structurally organized in a manner that allows improved protein binding, though we have not formally tested this hypothesis within this study.
IAVs can reassort and generate novel and potential pandemic strains. We tested if the regions identified in IAV-PR8 were required for replication of a divergent H7N3 virus. Synonymous changes designed to disrupt the predicted RNA structure in the same vRNA regions of two separate segments in both IAV-H7N3 and IAV-pH1N1 were attenuating and suggest conservations of these structural elements. Future examination of NP-binding of viral RNA in diverse strains of IAV is likely to identify overlapping and distinct high and low-NP-binding regions. These RNA features may be required for all viruses as coordinating packaging elements, but additional regions act as strain or lineage-specific packaging enhancers. Further, these strain or lineage-specific RNA features, required for genome packaging, may act as potential determinants of reassortment outcomes. Prior experimental systems demonstrated that co-segregation of genome segments occurs during natural reassortment as well as lab-adaptation of virus strains to create new vaccines34,35. Finally, attenuation of genome packaging through silent, structural mutations has the potential to accelerate live attenuated vaccine production using native genome constellations to allow vaccination with all protein epitopes of a novel pandemic virus, without the need for master donor strains.
Madin–Darby Canine Kidney (MDCK) cells were maintained in minimal essential medium (MEM) with 5% fetal bovine serum (FBS, Biowest), MEM-vitamins (Gibco), l-glutamine (Gibco), and penicillin–streptomycin (Gibco). Human embryonic kidney cells (293-T) were maintained in Opti-MEM (Life Technologies) with 10% FBS, l-glutamine, and penicillin–streptomycin. MDCK and 293T cells were a kind gift from Dr. Richard Webby at St. Jude Children’s Research Hospital.
Eight bidirectional pHW2000 plasmids containing cDNA for A/Puerto Rico/08/1934 (H1N1), A/California/04/2009 (H1N1), or A/shorebird/Delaware/22/2006 (H7N3) were previously described35. Viruses were generated by transfection of all eight plasmids into co-cultures of 293T and MDCK cells (1 μg per plasmid) with polyethylenimine (8 μg total). Wild-type (herein IAV-PR8 (WT-PR8), IAV-pH1N1 (WT-pH1N1) or IAV-H7N3 (WT-H7N3) and mutant viruses were generated in the same manner with the exception of substituting individual plasmids harboring mutagenized DNA for the single indicated wild-type plasmid. The next day, the transfection mixture was removed and replaced with Opti-MEM containing MEM-vitamins, l-glutamine and penicillin–streptomycin. Forty-eight hours post-transfection, an additional 1 ml of the same media containing 1 μg ml−1 TPCK-Trypsin (Worthington) was added to the co-culture. Seventy-two hours after addition of TPCK-Trypsin, culture supernatant was collected and clarified by centrifugation (5 min, 350 × g). Viral stocks were generated by infection of MDCK cells in a T75 flask. Cells were washed once with PBS, and 200 μl of transfection supernatant was mixed with 25 ml of infection media (M0.1B) composed of MEM containing vitamins, l-glutamine, penicillin–streptomycin, 0.1% Bovine Serum Albumin (Gibco) and 1 μg ml−1 TPCK-Trypsin for 48 h. Stocks were aliquoted and stored at −80 °C until use, and all studies were conducted with passage 1 stocks following verification of mutant sequence identity. All viruses were generated at least twice independently. Sequences for primers utilized during mutagenesis of IAV plasmids are available in Supplementary Table 2.
IAV nucleoprotein PAR-CLIP and RNA-seq library generation
To identify interactions between viral RNA and IAV nucleoprotein, we adapted the protocol for PAR-CLIP21 coupled to next-generation sequencing to discern nucleotide resolution maps of protein–RNA interaction across the IAV genome. Confluent 293-T cells were infected at an MOI of 1 for 16 h in the presence of 100 μM 4-thiouridine (4-SU) and then cross-linked with ultraviolet light (310 nM, 500,000 μJ total energy, Boekel Scientific UV Crosslinker AH (115 V)). PAR-CLIP was performed essentially as described before21 with the exception of antibodies used to immunoprecipitate NP–RNA complexes: monoclonal antibodies HB65 (ATCC) or MAb8258 (EMD Millipore). On-bead Calf Intestinal Alkaline Phosphatase (CIP, NEB) treatment was used to dephosphorylate RNA ends followed by 5′ end-labeling with 32P catalyzed by T4 Polynucleotide Kinase (PNK, NEB). Briefly, protein–RNA complexes were separated on 4–12% SDS-PAGE gels and transferred to nitrocellulose membranes. 32P-labeled RNA was identified by autoradiography, excised, and extracted following proteinase K (New England Biolabs (NEB)) digestion. To account for potential bias introduced during adaptor ligation and sequencing, we also generated RNA-seq libraries using RNA extracted from uncross-linked cell lysates of influenza infected 293T cells from which RNA was isolated by TRIzol (Invitrogen) extraction, then 10 μg RNA was fragmented by Mg2+ at 95 °C for 12 min (NEB, Magnesium RNA Fragmentation Module). 32P-labeling of this RNA was performed in solution with T4 PNK (NEB), and size-selection of 10–100 nt fragments by Urea-Page (15%) was performed. In both cases, total RNA was precipitated with ethanol, and used to prepare Illumina sequencing libraries. 3′-OH RNA previously prepared by CIP treatment was ligated to a 3′ adaptor using T4 RNA Ligase 2, truncated K227Q (NEB), according to manufacturer’s instructions. The 5′ adaptor sequence contains NNN-degenerate nucleotides in addition to a sequence used for demultiplexing to facilitate collapsing of redundantly generated PCR products and ascertain the frequency of individual NP–RNA interaction events. This adaptor was ligated to the 5′-end of RNA using T4 RNA Ligase 1 (NEB). Following isolation of adaptor ligated and radiolabeled RNA, all subsequent library generation steps were identical. Independent PAR-CLIP or RNA-seq libraries were pooled and cDNA synthesized using a primer complementary to the 3′ adaptor (SuperScript III, Invitrogen). Libraries were amplified by PCR for 9–15 cycles using Phusion DNA polymerase (Phusion HF Mastermix, Thermo) and primers annealing to the 5′ and 3′ termini of the DNA that enable flow-cell binding. Size selection of libraries was conducted by extraction of amplicons from 6% urea gel electrophoresis, and then libraries were precipitated. Molarity of libraries was determined by qPCR (NEBNext, New England Biosciences) and size by Bioanalyzer (Agilent). Illumina sequencing on the HiSeq 2500 instrument (1 × 50 bp reads) was performed by the Genome Technology Access Center at Washington University in St. Louis.
All NGS data were analyzed on the Washington University in Saint Louis School of Medicine’s McDonnell Genome Institute (MGI) cluster using publically available analysis programs (FastX toolkit, Bowtie, and SAMtools) and in-house scripts21. PAR-CLIP and RNA-seq data were generated from four independent experiments. For PAR-CLIP and RNA-seq libraries we used an analysis pipeline that collapsed unique barcoded reads, removed adaptors, and aligned them to the viral genome. Strandedness was determined post-alignment and all subsequent analysis was performed on negative-sense viral genomic RNA (vRNA). For reads that mapped to the viral RNAs, we normalized the number of reads per nucleotide to the total number of reads per genome segment to yield a normalized coverage ratio for both PAR-CLIP and RNA-seq libraries. We then compared the normalized coverage for each preparation at nucleotide resolution using an unpaired t test with False Discovery Rate (FDR) correction (Benjamini–Hochberg)36. vRNA nucleotides with a FDR minima Q < 0.01 and fold-change > 3 were identified and these regions were extended to the final nucleotide of each regionm, where Q ≤ 0.1. These areas represent low-NP or high-NP-binding regions of interest (ROI). ROI ≥ 18 nt were subjected to RNA structure analysis with mFold, RNAfold, and vsFold5 to determine computationally the theoretical minimal free energy (ΔG) of each region as well as the potential secondary or tertiary RNA structure formation37,38. Nucleotide composition of ROIs was determined by calculating the percent of A, G, C, or U in each region and comparing them to the percent of each nucleotide in the IAV genome. PAR-CLIP data sets were compared to RNA-seq data sets at each nucleotide position on a per-segment basis and positions using unpaired t test with FDR correction as above with a threshold of Q = 0.05. Sites of statistically enriched transition in PAR-CLIP data sets were tabulated and the sequence distance between these positions was calculated. Transition events with a step-size of 1, i.e. sequential nucleotides both with high transition rate, and were excluded from analysis because they likely represent single NP–RNA interactions of adjacent 4-SU nucleotides in vRNA. Transition rate for four PAR-CLIP libraries were averaged prior to distance calculation.
Generation of IAV containing altered RNA structures
Mutant selection was performed after analysis of open reading frames to assess potential nucleotide degeneracy while retaining coding sequence. Subsequently, we selected five underrepresented ROIs in four segments (1, 2, 5, and 8) for functional assessment of manipulating predicted RNA structures on viral replication. We also identified four regions in segment 5, amenable to extensive silent mutagenesis that were either highly bound or represented at the same frequency in PAR-CLIP and RNA-seq data sets. We introduced between 2 and 7 synonymous mutations simultaneously in silico to the identified regions and reassessed structural stability or pseudoknot formation using the same structure prediction programs. We selected variant codon combinations that would disrupt or maintain the predicted vRNA structure but not change the encoded amino acid, alter codon usage, or disrupt alternative reading frames or splicing events. Once predicted destabilizing mutations were identified, mutant viruses were generated. Mutations were introduced into pHW2000 bidirectional plasmids by inverse PCR with primers (see Supplementary Table 2) including selected mutations and unique ligation sites. PCR products were gel extracted and digested with either BsmbI (New England Biolabs, (NEB)) or AarI (Thermo Fisher Scientific) restriction enzymes and DpnI (NEB) to remove residual parent plasmid. Digested PCR products were PCR purified and ligated using Instant Sticky End Ligase (NEB). Ligation products were transformed into E. coli, plated on selective LB Agar. Colonies were selected and grown overnight in LB broth and selective antibiotics. All plasmids were grown in 200 ml LB Broth prior to preparation by Qiagen HiSpeed Endotoxin-Free MaxiPrep and verified by Sanger Sequencing (Genewiz).
Influenza A virus focus-forming assay
Six-well plates of MDCK cells were plated and inoculated with 1 ml of 10-fold serially diluted virus stock for 1 h in M0.1B. After 1 h, the inoculum was removed, and replaced with an overlay of 1% Low Melting Point Agarose in MEM supplemented with 1 μg ml−1 TPCK-Trypsin. Seventy-two hours post infection cells were fixed in 5% formaldehyde, permeabilized with 0.1% saponin in HBSS, and stained for NP protein with 1 µg ml−1 biotinylated anti-NP (mAB8258b, Millipore). Foci were visualized by addition of HRP-conjugated streptavidin and TruBlue streptavidin substrate and imaged on a Biospot reader (Cellular Technology Limited). Individual foci were counted and area was calculated using the Analyze Particles extension within Fiji39. The area of the foci formed by mutant viruses was normalized to the average of WT-PR8 foci per experiment. A minimum of 60 foci was analyzed per virus.
We infected 293T cells in the presence or absence (mock) of 4-SU with WT-PR8 (MOI of 1) for 16 h. Briefly, 293T cells were seeded on glass coverslips coated with poly-d lysine overnight. Cells were infected with WT-PR8 for 16 h then fixed with 4% methanol-free PFA in PBS (pH 7.4), washed with PBS, permeabilized with saponin, then NP staining was performed using anti-NP MAb HB65 (1 µg ml−1) and goat-anti-mouse Alexa-488 (Invitrogen). Nuclei were counterstained with DAPI. Coverslips were mounted to slides with ProLong® Diamond Antifade Mountant (Molecular Probes). To determine if viral protein production was impacted by synonymous mutations, we utilized confocal microscopy to visualize the distribution of NP during infection as a marker of viral protein production and a proxy for trafficking of vRNPs. MDCK cells were infected with WT-PR8 and indicated mutant viruses (MOI of 0.2) for 8 h, then fixed and permeabilized as before. NP staining was performed as above using anti-IAV-NP MAb HB65 and Goat-anti-Mouse Alexa-488 (Invitrogen). Slides were imaged with Zeiss LSM 880 Confocal Laser Scanning Microscopy with Airyscan and analyzed with Zeiss Zen Black Software performed within the Molecular Microbiology Imaging Facility at Washington University in Saint Louis.
Influenza A virus reporter assay
To assess the impact of silent mutations on polymerase complex activity, pHW2000 plasmids encoding WT or mutant PB2, PB1, PA, and NP were utilized in a dual-luciferase reporter assay as previously described35. A vRNA-like firefly luciferase reporter plasmid and a Renilla luciferase expression plasmid also were included. Briefly, 293T cells were seeded into 24-well plates and transfected with equal amounts of all six plasmids (500 ng DNA total) in Opti-MEM containing TransIT-LT1 (Mirus). Cells were maintained at 37 °C for the duration of the experiment. Forty-eight hours later, cells were lysed for analysis of luciferase activities (Promega). Each combination of polymerase proteins (set of plasmids) was examined in duplicate. The relative light units (RLU) of firefly luciferase activity were normalized to the RLU for Renilla luciferase activity within the same sample to account for differences in transfection efficiency between wells and experiments. The activity of each plasmid set containing a mutant segment was normalized to the activity of WT-PR8.
To determine the amount of NP generated at a fixed time during infection, we infected 2 × 105 MDCK cells (MOI of 0.2). Eight hours post-infection, cells were collected and fixed. Intracellular staining of IAV antigen was performed as above using an anti-IAV NP primary antibody (HB65) (1 µg ml−1) and an Alexa-488-conjugate goat-anti-mouse secondary antibody (Invitrogen). The mean fluorescence intensity (MFI) of NP+ cells was plotted and calculated from two experiments performed in duplicate. Co-expression of NP and M in MDCK cells was determined 16 hpi with an MOI of 5 and 0.05. Infected cells were fixed and stained as above with the additional staining step of utilizing mouse-anti-M primary antibody (M2-1C6) (2 µg ml−1) and goat-anti-mouse Alexa-647 secondary. A second intracellular staining step utilizing biotinylated mouse-anti-NP (mAb8258b) (1 µg ml−1) and Alexa-488 conjugated streptavidin was then performed. The frequency of co-expression was calculated by determining the number of cells expressing NP or M as well as those expressing both. The percentage of infected cells co-expressing NP and M was calculated by dividing co-expressing cells by all cells expressing one or more viral protein as previously described23. Analysis of viral proteins was determined using a flow cytometer and FlowJo software (Tree Star).
Multi-step replication of influenza A virus
MDCK cells (2 × 105) were seeded in 24-well plates and inoculated the next day with 200 TCID50 of IAV. Cells were washed once with PBS before addition of inoculum in M0.1B and incubated for 1 h at 37 °C. Subsequently, the cells were washed twice with PBS and 1 ml of M0.1B supplemented with 1 μg ml−1 TPCK-Trypsin was added to each well. Culture supernatants were collected at 24 and 48 hpi and the amount of infectious virus was quantified by titration on MDCK cells.
Titration of influenza A virus (TCID50)
Confluent monolayers of MDCK cells were grown overnight in 96-well tissue culture plates. The next day, the cells were washed with PBS and inoculated with 10-fold serial dilutions of culture supernatant or lung homogenate for 1 h in M0.1B at 37 °C. After 1 h, the inoculum was removed and replaced with M0.1B supplemented with 1 μg ml−1 TPCK-Trypsin and incubated for 72 h. Presence of virus was determined by hemagglutination assay using 0.5% turkey red blood cells. TCID50 was determined by the Reed–Muench method40.
Infection of mice with influenza viruses
Male C57BL/6 J mice (5–6 weeks of age) were bred in-house in a barrier facility at Washington University School of Medicine, St. Louis, MO or purchased from Jackson Laboratories. Mice received food and water ad libitum and all experiments were conducted in accordance with rules of the Institutional Animal Care and Use Committee. Mice were anesthetized with isoflurane in an airflow chamber and then inoculated intranasally with 30 μL of sterile PBS containing 1000 TCID50 of WT-PR8 or the indicated mutant virus. Forty-eight hours post-infection, lungs were collected and homogenized in 1 ml M0.1B, cleared by centrifugation at 1200 × g for 5 min, then stored in aliquots at −80 °C. Viral titers from lung homogenates were determined by TCID50 assay.
Segment abundance RT-qPCR
MDCK cell derived stocks of WT-PR8 or mutant viruses were clarified by centrifugation at 1200 × g for 5 min, passed through a 0.22 µM filter, and pelleted on 30% sucrose cushion by ultracentrifugation (Beckman SW32ti swinging bucket rotor, 27 K RPM, 4 °C for 90 min). Pelleted virus particles were directly resuspended in 350 μl TRK lysis buffer, and RNA purified immediately and eluted in 30 μl DEPC H2O (Total RNA Kit I, Omega). cDNA was synthesized from 5 µl of RNA with SSIII Reverse transcriptase (Invitrogen) and a vRNA specific primer22. Total cDNA was diluted 1:10,000 and used to quantify each of the 8 genome segments by SYBR Green qPCR (PowerUpTM SYBR® Green Master Mix) with primer pairs previously published22. Relative abundance of each genome segment was calculated as before, except with normalization to Segment 1 or 7 depending on the virus assessed.
RNA thermal denaturation experiments
The RNA oligomers corresponding to PB1497–561, PB1497–561:A, and PB1497–561:B (Supplementary Table 2) were synthesized commercially (Integrated DNA Technologies). RNA was diluted in buffer (100 mM NaCl and 50 mM KCl) to a final concentration of 10 µM, incubated at 95 °C for 5 min and flash cooled on ice to induce folding. Unfolding of RNA secondary structure was monitored by CD spectroscopy at 210 and 260 nm on a Chirascan CD spectrometer (Applied Photophysics)41. RNA was heated from 5 to 95 °C at a rate of 1 °C/min and CD readings collected every 2 °C. A linear, least-squares program was used to fit the transition region of thermal melting curves. By definition, the melting temperature of nucleic acid is the temperature at which 50% of all nucleic acid becomes single-stranded for a single cooperative transition24. The ellipticity at which half of the RNA is denatured was calculated using the equation shown below and modified from (42):
where, Fd is the fraction of denatured nucleic acid, θn the minimum ellipticity at 210 nm or the maximum ellipticity at 260 nm, θd the maximum ellipticity at 210 nm or the minimum ellipticity at 260 nm, and θobs the observed ellipticity at Fd. The ellipticity, θobs, when Fd = 0.5 was calculated and used to calculate Tm from the linear fit of the transition region. For comparisons between different RNA samples, the first derivative of the ellipticity, θobs, the change in molar ellipticity as a function of temperature (dθ/dT), was calculated and plotted.
All statistical analyses were performed using GraphPad Prism 7.0. For comparison of PAR-CLIP and RNA-seq data sets, we used multiple unpaired t tests with the Benjamini–Hochberg correction to identify areas in which these sequencing preparations were statistically different from each other (Q < 0.1 and Q < 0.01). Transition-distance was determined using FDR (Q < 0.05). For analysis of the focus-forming assay, luciferase assay, and lung viral titers, we used one-way ANOVA with multiple comparison corrections (Kruskal–Wallis test). TCID50 per HA-unit was analyzed using an unpaired t test.
The unprocessed PAR-CLIP and RNA-seq files have been uploaded to the NCBI Sequence Read Archive (SRA, SUB3042681). All other data supporting the findings of this study are available within the article and its Supplementary Information files, or are available from the authors upon request.
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We thank the following colleagues for comments on experiments comprising this manuscript: Michael Diamond, MD, David Wang, PhD, Daisy W. Leung, PhD, and Deborah Lenschow, MD and Anshu Gounder all of Washington University in Saint Louis. Additionally, Traci Bricker and Christopher Edwards provided technical support. Chris Brooke provided anti-M1 hybridoma, M2-1C6. NIGMS training grant 5T32GM007067 and NIAID ID Training Grant 2T32AI007172, and the Victoria Fraser Infectious Disease Research Fellowship provided funding to GW. This study was funded in part by the National Institute of Health (NIH) grants P01AI120943 (G.K.A) and R01-AI118938 (A.C.M.B.).
The authors declare no competing financial interests.
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Williams, G.D., Townsend, D., Wylie, K.M. et al. Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals RNA features required for replication. Nat Commun 9, 465 (2018). https://doi.org/10.1038/s41467-018-02886-w
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