Fig. 4 | Nature Communications

Fig. 4

From: One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains

Fig. 4

Characterization of synthetic promoter driven aromatic polyesters. a Scheme of the PhaAB expression cassette. Analysis of d-phenyllactate monomer fraction b and differential scanning calorimetry (DSC) analysis c of copolymers produced by XB201TBAL strain expressing PhaAB under the synthetic Anderson promoters (BBa_J23100, BBa_J23105, BBa_J23114, BBa_J23109, and BBa_J23103) followed by overexpression of AroGfbr, PheAfbr, FldH, HadA, and PhaC1437. dg Grazing-incidence X-ray scattering (GIXS) patterns of poly(3HB) d, poly(16.8 mol% d-lactate-co-80.8 mol% 3HB-co-1.6 mol% d-phenyllactate-co-0.8 mol% d-4-hydroxyphenyllactate) e, poly(80.8 mol% 3HB-co-35.5 mol% d-phenyllactate) f, and poly(52.1 mol% 3HB-co-47.9 mol% d-phenyllactate) g. Colors indicate the intensity counts ranging from 0 (blue) to 105 (red). h In-plane line cut of GIXS patterns of each polymer (d black; e blue; f orange; g yellow). i Dual cantilever beam (DCB) test of polymers. *P < 0.05 and **P < 0.01, by two-tailed t-test. Error bars represent s.d. of n = 3 technical replicates

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