Fig. 7 | Nature Communications

Fig. 7

From: Macrophages orchestrate breast cancer early dissemination and metastasis

Fig. 7

Intra-epithelial macrophage numbers in human DCIS lesions negatively correlate with E-cadherin levels. Human adjacent healthy (a) and DCIS tissue (b) was stained against CD68 (macrophages) and smooth muscle actin (SMA). Bar: 75 μm; zoom factor 4.75x. c Plot shows mean±SEM of the percentage of ducts containing intra-epithelial macrophages (IEMs) from 7 healthy and 10 DCIS patients; each dot represents one patient; statistical analysis: Mann–Whitney test. Sections from human DCIS tissue were stained against CD68 (macrophages) and E-cadherin (d, e, bar: 75 μm; zoom factor 4.1x). E-cadherin pixel intensity was quantified in regions of individual cell junctions and medians for individual patients were quantified. Plot f depicts mean E-cadherin intensity throughout DCIS lesions of individual patients with low or high intra-epithelial macrophage (IEM) numbers (total patient number N = 12; statistical analysis: Mann–Whitney test—SEM shown)

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