Fig. 3 | Nature Communications

Fig. 3

From: Macrophages orchestrate breast cancer early dissemination and metastasis

Fig. 3

Macrophage depletion during pre-malignant stages prevents early cancer cell dissemination. Twenty-week-old pre-malignant MMTV-HER2 mice were treated with the anti-CSF1R ASF98 antibody and animals were harvested after 2 weeks with no signs of invasive carcinoma (a). Analysis of H&E staining of mammary gland sections confirmed the absence of invasive lesions (b, c; bars: 100 μm). E-cadherin expression in whole mammary glands was determined (d; mean±SEM; control 7, anti-CSF1R 6 animals; statistical analysis: Mann–Whitney test) and by immunofluorescent staining against E-cadherin in mammary gland sections (e, f; bars: 10 μm). E-cadherin signal intensity was measured in individual regions of cell junctions in three animals per group (g). P values were calculated with 95% confidence interval by Mann–Whitney test. Early circulating cancer cells (eCCCs) were quantified in mice per group as the amount of HER2 and CK8/18+ eCCs/ml peripheral blood. Values were normalized to the mean of controls and are shown as mean±SEM (h) depicts normalized mean±SEM; seven animals per group; statistical analysis: Mann–Whitney test. Disseminated HER2+ eCCs were quantified in lung sections (i, j; bars: 25 μM) and were quantified as the average of HER2+ cells per 100 randomly chosen microscopic fields (k). Mean±SEM are shown; statistical analysis: Mann–Whitney test