Fig. 5 | Nature Communications

Fig. 5

From: 133p53 isoform promotes tumour invasion and metastasis via interleukin-6 activation of JAK-STAT and RhoA-ROCK signalling

Fig. 5

∆122p53-expressing PDAC cells have constitutively active JAK-STAT and RhoA-ROCK signalling pathways. a Quantitation of invasive index and representative images of pan-cytokeratin s-stained sections of PDAC/vector (Vo) and PDAC/Δ122 (Δ) cells invading through collagen matrix contracted by TIFs treated with either control solvents, Pan-JAK inhibitor P6, the ROCK inhibitors Y-27632 and H1152, and the RhoA inhibitor TAT-C3, respectively. b Quantitation and representative images of pSTAT3-stained sections of invading PDAC cells upon treatment with the above inhibitors. c Quantitation and representative images of pMYPT1-stained sections of invading PDAC cells upon treatment with the above inhibitors. Scale bar = 100 μm. The bars represent the mean and error bars represent ±s.e.m of n = 3 repeats with 3 biological replicates each. p-values were determined using unpaired t-tests. p < 0.05 was considered significant. *p < 0.05, **p < 0.005 and ***p < 0.0005 are designated, respectively

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