Fig. 4 | Nature Communications

Fig. 4

From: The dynamic dimer structure of the chaperone Trigger Factor

Fig. 4

Domain contacts in the full-length TF dimer. Result of PRE experiments with a paramagnetic spin label attached to one of the positions S30, V49, S61, S72, A223, and E326 in full-length TF measured in sample buffer (20 mM K-phosphate pH 6.5, 100 mM KCl, 0.5 mM EDTA) at 25 °C and 700 MHz. The peak volume ratio between oxidized and reduced samples from 2D [15N,1H]-TROSY is plotted against the residue number. For visualization purposes, a value of 0.15 is shown for the peaks that were broadened beyond detection in the paramagnetic sample. Data are shown only for non-overlapping resonances. The black line outlines the PRE effect observed for each mutant. The orange-shaded regions correspond to intermolecular PRE and the green shaded to intramolecular effects, as expected from the monomeric crystal structure (PDB 1W26). The colored bars on top show the sequence domain organization as in Fig. 1

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