Fig. 3 | Nature Communications

Fig. 3

From: The dynamic dimer structure of the chaperone Trigger Factor

Fig. 3

Determination of the lifetime of the TF dimer. a Experimental scheme. At the onset of the experiment (t = 0), separately produced samples of [U-15N,2H]-labeled (green) and randomly spin-labeled (brown) TF are mixed in equal ratio in sample buffer (left). The NMR signal intensity is then monitored in real time t during the equilibration to the end point (right). NMR signals of protomers bound to a spin-labeled protomer are reduced in intensity by the intermolecular PRE, symbolized by light green color. b Intensity of 15N-filtered NMR signals, I Δ, rel, following the experimental scheme in a. The lifetime of the TF dimer is obtained by non-linear least-square fits (lines) to the data (dots). See Supplementary Note 1 for mathematical details. The experiment was performed at five temperatures in the range 15–35 °C, as indicated

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