Fig. 2 | Nature Communications

Fig. 2

From: The dynamic dimer structure of the chaperone Trigger Factor

Fig. 2

Localization of pairwise interaction sites on individual TF domains. a NMR titration of unlabeled SBD–PPD to 100 μM [U-15N] RBD in sample buffer (20 mM K-phosphate pH 6.5, 100 mM KCl, 0.5 mM EDTA) at 25 °C and 700 MHz. b NMR titration of unlabeled RBD to 250 μM [U-2H,15N] SBD–PPD in sample buffer at 25 °C and 700 MHz. c Chemical shift perturbation of the amide moieties observed in the titrations: [U-15N] RBD + SBD–PPD (top left), [U-15N] RBD + SBD (bottom left), [U-2H,15N] SBD–PPD + RBD (top right), and [U-2H,15N] SBD + RBD (bottom right). Light-shaded bars represent peaks undergoing line-broadening. Dashed lines are plotted at defined thresholds (mean value of the chemical shift perturbations plus one time and plus two times the standard deviation corrected to zero). d Significant chemical shift perturbations plotted in TF crystal structure (PDB 1W26). The affected residues are plotted with color gradient from light to dark for peaks with chemical shift changes above the threshold and that broaden beyond detection, respectively

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