Fig. 5 | Nature Communications

Fig. 5

From: Metas-Chip precisely identifies presence of micrometastasis in live biopsy samples by label free approach

Fig. 5

Cytopathological and immunohistochemical images of the lymph node aspirated from a known metastatic sample (ID 43) positively scored by Metas-Chip in comparison with the similar assays from two suspicious aspirated samples (ID 68 and 69) negatively scored in Pap stain, but positively scored by Metas-Chip and IHC. a Cancer cells with large hyperchromic nucleus present metastatic carcinoma, meanwhile no trace of malignant cells could be observed in (e, i). Expression of PCK in the cancer cells are observable in metastatic (b) and one of the suspicious patients (f). Also the expression of PCK is suggestable in lymph of other suspicious patient (j). Expression of CK7 was positive in patient ID 43 (c) while it is negative in patient ID68 (g) and ID69 (k). Positive expression of EMA is observed in known metastatic (d) and suspicious patients (h, l). At least one IHC marker was positive in the suspicious patients who had been positively scored by Metas-Chip. Expression of metastatic epifluorescent marker (anti-proMMP2) on malignant cells invaded HUVEC trap. m Optical microscopy and (n) epifluorescence image of live single-HUVEC trap (stained by Dil: yellow) and breast cancer cells tagged for MMP2 (by anti-proMMP2: green) proteins presented in the surface of metastatic cells. Both of the cancer cells could be observed in optical images, but just one of them expressed the marker as active metastatic cells could be traced in epifluorescent image. Epifluorescent images of metastatic cell (expressed the marker) 90 min after invading the individual HUVEC trap showed entrance of the metastatic cell to vascular barrier . The scale bars are 25 µm in length

Back to article page