Fig. 3 | Nature Communications

Fig. 3

From: Metas-Chip precisely identifies presence of micrometastasis in live biopsy samples by label free approach

Fig. 3

Capture of metastatic cells in SLN samples of a patient with metastatic cancer by both Metas-Chip and pathological process. a Representative images of two live metastatic cells detached themselves from the isolated lymph of the patient with metastatic breast cancer and attacked two individual single-HUVEC-sensing traps. b Their invasion induced sharp reduction in electrical response of the traps by retracting the HUVECs from the sensing electrodes, which could be traced in time-lapse optical images (c) and simultaneous schematics (d). H&E and immunohistochemical characterization of the patient’s SLN: (e) Images of a lymph region stained with hematoxciline and eosine. Nest of tumor cells with hyperchromic nucleus could be observed. This would indicate that the lymph node has been metastasized by invasive ductal breast carcinoma. (f) Pan cytokeratins and (g) vimentin expression in the sentinel lymph node of the patient with metastatic breast carcinoma. High reactivity of the cells with the markers (brown-yellow faint colors in the images) indicates the sharp involvement of the lymph with tumor cells. The scale bars for a, c, d, f and g are 25 µm in length and the scale bar for e is 100 μm in length

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