Multiyne chains chelating osmium via three metal-carbon σ bonds

Although the formation of metal–carbon σ bonds is a fundamental principle in organometallic chemistry, the direct bonding of one organic molecule with one metal center to generate more than two metal–carbon σ bonds remains a challenge. Herein, we report an aromaticity-driven method whereby multiyne chains are used to construct three metal–carbon σ bonds in a one-pot reaction under mild conditions. In this method, multiyne chains act as ligand precursors capable of chelating an osmium center to yield planar metallapolycycles, which exhibit aromaticity and good stability. The direct assembly of various multiyne chains with commercially available metal complexes or even simple metal salts provides a convenient and efficient strategy for constructing all carbon-ligated chelates on the gram scale.

In recent years, our research has focused on the construction of as many metal-carbon σ bonds as possible between a carbon ligand and a metal center, achieving a series of conjugated carbon-ligated multidentate chelates, termed carbolong complexes, with novel structures and unique properties [39][40][41][42][43] . All of these complexes contain three or more metal-carbon σ bonds; however, these metal-carbon σ bonds could only be constructed step by step.
Aromaticity-driven cyclization reactions have been widely used in synthetic chemistry owing to the relatively lower energies of the aromatic products compared to their non-aromatic or antiaromatic analogs. Numerous aromatic hydrocarbons have been constructed using this method. As shown in Fig. 1a, one of the most prominent examples of this chemistry is the transition metal-catalyzed [2 + 2 + 2] cycloaddition reactions of alkynes, which can form three carbon-carbon σ bonds in a one-pot reaction to obtain benzene and its derivatives 44 . Thus, we considered whether the direct construction of three metal-carbon σ bonds could also be achieved through an aromaticity-driven process. Herein, we present an efficient strategy involving aromaticity-driven processes in a one-pot reaction to achieve stable multidentate complexes containing three metal-carbon σ bonds (Fig. 1b). The direct reaction of multiyne chains with a commercially available metal complex (OsCl 2 (PPh 3 ) 3 ) or even a simple metal salt (K 2 OsCl 6 ) affords all carbon-ligated chelates with both single and multiple metal-carbon bonds under ambient conditions on the gram scale. The promising synthetic potential of this method is evidenced by the extension of the multiynes to produce diverse chelate scaffolds.

Results
Synthesis of CCC-type chelates with three M-C σ bonds. We first designed and prepared the carbon ligand precursor L1 ( Fig. 2a; see Supplementary Methods for synthesis details). L1 is a triyne chain containing two functional modules (an acetenyl unit and a 3hydroxyl pentadiynyl unit) connected by a flexible linker. As shown in Fig. 2a, the treatment of L1 with OsCl 2 (PPh 3 ) 3 and PPh 3 in dichloromethane under air at room temperature (RT) for 1 h resulted in the formation of CCC-type chelate 1 with a yield of 82%.
Chelate 1 was characterized by nuclear magnetic resonance (NMR) spectroscopy. The 13 C NMR spectrum shows a typical chemical shift of the carbyne carbon in 1 at 321.57 parts per million (ppm). The downfield 1 H NMR chemical shifts (H7 at 13.14 ppm and H3 at 7.64 ppm) appear to be diagnostic of the presence of an aromatic framework, which is common for aromatic metallacycles [26][27][28][29][30][31][32][33][34][45][46][47] . Single-crystal X-ray analyses indicated that a tridentate CCC-type ligand was attached to the osmium center by three metal-carbon σ bonds (Fig. 2b), confirming that the combination of L1 with the osmium substrate led to the conversion of five sp carbons and one sp 3 carbon to six sp 2 carbons. The Os-C1 bond length (1.840(7) Å, where the value in parentheses represents the standard deviation) is at the high end of the range for Os≡C triple bonds (1.612-1.856 Å). However, the other two Os-C bond lengths (i.e., 2.108(7) Å for Os-C4 and 2.040(8) Å for Os-C7) are within the range of the  Os-C single bonds (1.921-2.195 Å) of vinyl osmium complexes (these ranges are based on a search of the Cambridge Structural Database, CSD version 5.38, in November 2016). The carbon-carbon bond distances within the metallacycles (1.366 (10)-1.406(10) Å) lie between the typical single and double carbon-carbon bond distances. The metallabicycle is nearly coplanar, as reflected by the small mean deviation from the least-squares plane (0.020 Å). The planarity of the metallacycles and the carbon-carbon bond distance equivalency indicate extensive electronic delocalization within the osmabicycle ring in 1. All the salient features of 1 compare well with those of previously reported aromatic osmapentalynes 39 . Osmapentalynes and related osmium chelates with multidentate carbon ligands have attracted considerable attention as novel aromatic molecules with unique properties, especially in the area of photophysics [39][40][41][42][43]48 . However, previous methods reported for the synthesis of these chelates with multidentate carbon ligands require the use of an unstable osmacycle precursor via standard Schlenk techniques [39][40][41][42][43]48 . By contrast, triyne L1 can lead to an osmapentalyne in a one-pot synthesis under an atmosphere of air and from commercially available metal source.
The facile synthesis of 1 using this method demonstrates that it is highly efficient for constructing complexes with three metal-carbon σ bonds via the cyclization of multiyne chains with metal species. To explore the generality of our strategy, we prepared multiynes with different linkers. Similar to L1, L2-L4 were able to chelate an osmium center via three metal-carbon σ bonds in a one-pot reaction to achieve CCC-type chelates 2-4, respectively, in high yield (Fig. 2a). These results demonstrate that our method can be applied to a broad range of substrates.
Extension of the multiyne platform. The efficient ligating ability of L1-L4 inspired us to investigate whether an extension of the multiyne chain will provide tetradentate carbon-ligated chelates and, if so, how the synthesis is achieved.
As shown in Fig. 3a, the reaction of tetrayne L5 with OsCl 2 (PPh 3 ) 3 in air afforded complex 5 with a yield of 73%. The crystal structure of 5-PF 6 (the derivative of 5 with PF 6 as the counteranion) reveals that 5 has a fused metallapentalene ARTICLE structure with the metallacyclopropenylidene moiety, which is a unique planar tetradentate CCCC-type chelate (Fig. 3b) (5) Å) is a typical exocyclic double bond. By contrast, the C-C bond distances within the tricyclic skeleton are all between the carbon-carbon single-and double-bond distances, suggesting that there is π-electron delocalization within the fused metallacycle (see Supplementary Fig. 1 for resonance structures). Consistent with the experimental observations, theoretical calculations (natural bond orbital analyses) also indicated that 5 is a tetradentate CCCC-type chelate ( Supplementary Fig. 2). It is worth noting that the eight C(sp) and one C(sp 3 ) atoms of L5 were completely converted into nine C(sp 2 ) atoms and were incorporated into the final planar conjugated skeleton. Interestingly, the formation of 5 was also achieved by reacting L5 with the inorganic salt K 2 OsCl 6 under air (Fig. 3a). Although the reactions of triynes L1-L4 with inorganic salts did not work, the reaction of tetrayne L5 with K 2 OsCl 6 indicates that the direct combination of inorganic salts with multiynes could be a readily accessible strategy. This approach may provide opportunities for the use of multiyne chains as carbon ligand precursors; that is extending multiyne chains could enhance their chelating ability, which would lead to the synthesis of useful chelates in an efficient manner.
Multiyne chains with allene groups were also compatible with our strategy. As shown in Fig. 4a, the reaction of L6 (or L7) with OsCl 2 (PPh 3 ) 3 led to the formation of complex 6 (or 7) with a yield of 51% (or 81%). X-ray crystallography analyses confirmed that both 6 ( Fig. 4b) and 7 (Supplementary Fig. 6) were tetradentate CCCC-type chelates. The main difference between 5 and 6/7 is the hybrid form of C8, which is sp 2 -hybridized in 5 but sp 3 -hybridized in 6 and 7.
In addition to all carbon-ligated chelates, chelates with hetero coordination atoms can also be constructed via the cyclization reactions of multiyne chains with metals. As shown in Fig. 5, the reaction of the phenolic group substituted triyne L8 with OsCl 2 (PPh 3 ) 3 afforded the tetradentate CCCO-type chelate 8 with a yield of 71%. 8 was characterized by NMR spectroscopy and all of its characteristic chemical shifts of protons, carbons, and phosphorus were consistent with those of recently reported values for osmapentalenofuran 50 . The structure of 8 was further confirmed by high-resolution mass spectrometry (HRMS), and elemental analyses. It is noteworthy that 8 represents a rare example of a planar tetradentate CCCO-type chelate 50 .
DFT studies of the aromaticity of 1-8. Consistent with their structural aromatic characteristics, 1-8 exhibit good stability (all of them stable in air at RT). Density functional theory (DFT) calculations were performed to evaluate their aromaticity. Taking 1 as an example, the calculated aromatic stabilization energy (ASE) results are comparable to the values reported for other metallaaromatics (Fig. 6a) 39,[49][50][51][52][53][54] . We also calculated the nucleus-independent chemical shift (NICS) along the z-axis at 1 Å above the ring critical point (NICS(1) zz ; the average value was used when the environments above and below the ring centers were not equivalent) of model compound 1′, which we simplified by replacing the PPh 3 groups with PH 3 groups (Fig. 6b) [55][56][57] . Both the considerable ASE value and negative NICS values suggest that the ring systems in complex 1 are aromatic. The anisotropy of the induced current density (AICD) method 58,59 was applied to visualize the electron delocalization within the metallacycles (Fig. 6c). The aromatic character was further confirmed by the obvious diatropic ring current (clockwise vectors) within the metallabicyclic moiety of model osmapentalyne 1′. are also in good agreement with their aromatic natures. Thus, we propose that the aromaticity of the final products is crucial for the assembly of multiynes with osmium in a one-pot reaction.

Discussion
To characterize the aromaticity-driven processes for multiynes chelating osmium, we performed DFT calculations. The computed free-energy profile of the key steps in the reaction of L1 with the osmium center to yield the final σ-bonded CCC-type chelate 1 is shown in Fig. 7   kcal/mol from B1 to D1). Finally, an acid-mediated aromatization can yield the final energetically favorable aromatic product 1 with three metal-carbon σ bonds, which is substantially more stable (by 21.2 kcal/mol) than E1. DFT calculations clearly show that the aromatic product 1 is more stable (more than 17 kcal/ mol) than all the intermediates, which provides a thermodynamic driving force for the formation of three metal-carbon σ bonds. The rationality of the aromaticity-driven mechanism was further verified by the isolation of a key intermediate for the synthesis of CCC-type chelate 4. As shown in Fig. 8, when the reaction of L4 with OsCl 2 (PPh 3 ) 3 was carried out in a mixed solvent of dichloromethane and tert-butanol (3/1, v/v) at 0°C for 0.5 h, complex 9 was isolated with a yield of 77%. Although 9 is labile in common organic solvents, it can be stable in tetrahydrofuran for weeks. Single-crystal X-ray diffraction (Fig. 9) reveals that 9 is an alkynyl-coordinated osmium vinyl complex with a free terminal C≡C bond, which is consistent with the intermediate B1 shown in Fig. 7. Other related metal vinyl complexes have also been isolated as intermediates that were demonstrated to be highly reactive 60,61 . Complex 9 could convert to osmapentalyne 4 when the reaction temperature was increased to RT. As monitored by in situ NMR, the conversion of L4 to intermediate 9 and finally to chelate 4 further confirms that 9 is a key intermediate in the aromaticity-driven cyclization reaction (see Supplementary Fig. 21 for in situ 31 P NMR monitoring spectra). Attempts to capture the corresponding intermediates related to C1 and D1 were unsuccessful, probably due to the strong aromaticity driving force of the final product.
In summary, we developed a highly efficient multidentate carbon ligand platform that involves the use of multiyne chains for the preparation of metal chelates with three or more metal-carbon σ bonds in a one-pot reaction under mild conditions. The multiyne chains consist of two functional modules and a linker, we term which carbolongs. They can be used to react with a commercially available metal complex, or even a simple metal salt, affording all carbonligated chelates on the gram scale. Our theoretical and experimental studies revealed that aromaticity-driven processes play a substantial role in the high efficiency and robust characteristics of chelating metals with these multiyne chains. The application of this strategy to utilize various multiynes as a carbon ligand platform will potentially gain widespread use as a promising complement to existing techniques for the preparation of polycyclic organometallic complexes. Synthesis of tetradentate CCCC-type chelate 7. A dichloromethane solution (2 mL) of L7 (130 mg, 0.802 mmol) was added slowly to a green solution of OsCl 2 (PPh 3 ) 3 (550 mg, 0.525 mmol) and PPh 3 (692 mg, 2.64 mmol) in dichloromethane (20 mL). The reaction mixture was stirred at RT for 30 min to yield a brown solution. The solution was evaporated under vacuum to a volume of~2 mL and washed with Et 2 O (3 × 30 mL) to afford a brown solid. The solid was purified by flash chromatography on silica gel (eluent: 20:1 dichloromethane/methanol) to yield complex 7 as a yellow solid. Yield: 506 mg, 81%.
Computational details. To obtain the ASE values and elucidate the mechanism, we used the original structures without changing the PPh 3 groups to PH 3 groups to obtain a more reasonable estimation of the energy differences. All of these structures evaluated were optimized at the B3LYP/6-31G* level of DFT [62][63][64][65] with an SDD basis set 66 to describe P, Cl, and Os atoms; single-point energy calculations were then performed on the mechanism using the TPSS/6-31G* method 67 with the PCM solvation method in dichloromethane 68 . Because we used simplified structures in the other calculations, all of these structures were studied using a larger basis set. The B3LYP/6-311++G** level of DFT 62-65 with a double-ζ valence basis set (LanL2DZ) was used to describe the P, Cl, and Os atoms. Frequency calculations were performed to confirm the characteristics of all the calculated structures as minima. In all calculations, the effective core potentials (ECPs) reported by Hay and Wadt with polarization functions were added for P (ζ(d) = 0.34), Cl (ζ(d) = 0.514), Os (ζ(f) = 0.886) 69 . NICS values were calculated at the B3LYP-GIAO/6-311++G** level. All the above calculations were performed using the Gaussian 09 software package 70 . The AICD calculations were performed using AICD software 58 .
Data availability. Data relating to the X-ray crystal structures are available from the Cambridge Crystallographic Data Centre (CCDC) under deposition numbers CCDC 1506325 (1), CCDC 1506339 (5-PF 6 ), CCDC 1548457 (6), CCDC 1548468 (7), and CCDC 1548472 (9). These data can be obtained free of charge from the Cambridge Crystallographic Date Centre via www.ccdc.cam.ac.uk/data_request/cif. For the XYZ coordinates of the optimized structures in the DFT studies of the aromaticity and mechanism, see Supplementary Data 1 and 2. The authors declare that all other data supporting the findings of this study are available within the article and its Supplementary Information file.