Fig. 3 | Nature Communications

Fig. 3

From: The anthelmintic praziquantel is a human serotoninergic G-protein-coupled receptor ligand

Fig. 3

(R)-PZQ constricts mouse mesenteric vasculature. a Image of mesentery surrounding mouse intestine, showing blood vessel containing a schistosome worm-pair (inset). b Representative image of a surgically isolated piece of mouse mesenteric artery held under tension between two clamped wires. Scalebar, 1 mm. c Changes in tension triggered by varied manipulations. Left, sustained contraction of vessels in KPSS and 5-HT (10 µM). Right, tension in individual arteries incubated in the presence of vehicle (DMSO, open circle) or SB204741 (10 µM, closed circle), and challenged with (R)-PZQ (50 µM) or (S)-PZQ (50 µM). W, wash; complete media exchange. Preincubation of strips with SB204741 (10 µM) attenuated (R)-PZQ evoked vasoconstriction. d Cumulative data set from experiments reporting effects of ligands relative to KPS(S)-evoked contractile tension, concentrations of ligands: 5-HT (1 µM), (R)-PZQ (50 µM), (S)-PZQ (50 µM), SB204741 (10 µM). Replicate number for each measurement as indicated, where individual measurements reflect the response from a naïve artery isolated from different mice. Data are presented as mean ± s.d. Probability, p < 0.05 (*), p < 0.01 (**). e Schematic model (not to scale) depicting (R)-PZQ action on both parasite (contraction) and host vasculature (contraction, increased perfusion pressure) facilitating hepatic shift of worms

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