Oxidative rearrangement of (+)-sesamin by CYP92B14 co-generates twin dietary lignans in sesame

Murata, Jun; Ono, Eiichiro; Yoroizuka, Seigo; Toyonaga, Hiromi; Shiraishi, Akira; Mori, Shoko; Tera, Masayuki; Azuma, Toshiaki; Nagano, Atsushi J.; Nakayasu, Masaru; Mizutani, Masaharu; Wakasugi, Tatsuya; Yamamoto, Masayuki P.; Horikawa, Manabu

doi:10.1038/s41467-017-02053-7

Download PDF

Article
Open access
Published: 18 December 2017

Oxidative rearrangement of (+)-sesamin by CYP92B14 co-generates twin dietary lignans in sesame

Jun Murata ORCID: orcid.org/0000-0001-7867-330X¹^na1,
Eiichiro Ono²^na1,
Seigo Yoroizuka³^na1,
Hiromi Toyonaga²,
Akira Shiraishi¹,
Shoko Mori¹,
Masayuki Tera¹,
Toshiaki Azuma¹,
Atsushi J. Nagano^4,5,
Masaru Nakayasu⁶,
Masaharu Mizutani⁶,
Tatsuya Wakasugi³,
Masayuki P. Yamamoto³ &
…
Manabu Horikawa ORCID: orcid.org/0000-0003-3070-006X¹

Nature Communications volume 8, Article number: 2155 (2017) Cite this article

6491 Accesses
44 Citations
1 Altmetric
Metrics details

Subjects

An Author Correction to this article was published on 25 May 2018

This article has been updated

Abstract

(+)-Sesamin, (+)-sesamolin, and (+)-sesaminol glucosides are phenylpropanoid-derived specialized metabolites called lignans, and are rich in sesame (Sesamum indicum) seed. Despite their renowned anti-oxidative and health-promoting properties, the biosynthesis of (+)-sesamolin and (+)-sesaminol remained largely elusive. Here we show that (+)-sesamolin deficiency in sesame is genetically associated with the deletion of four C-terminal amino acids (Del4C) in a P450 enzyme CYP92B14 that constitutes a novel clade separate from sesamin synthase CYP81Q1. Recombinant CYP92B14 converts (+)-sesamin to (+)-sesamolin and, unexpectedly, (+)-sesaminol through an oxygenation scheme designated as oxidative rearrangement of α-oxy-substituted aryl groups (ORA). Intriguingly, CYP92B14 also generates (+)-sesaminol through direct oxygenation of the aromatic ring. The activity of CYP92B14 is enhanced when co-expressed with CYP81Q1, implying functional coordination of CYP81Q1 with CYP92B14. The discovery of CYP92B14 not only uncovers the last steps in sesame lignan biosynthesis but highlights the remarkable catalytic plasticity of P450s that contributes to metabolic diversity in nature.

Characterization of CYP71AX36 from Sunflower (Helianthus annuus L., Asteraceae)

Article Open access 04 October 2019

Maximilian Frey, Iris Klaiber, … Otmar Spring

Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria

Article Open access 04 February 2021

Gaohua Yang, Sen Hong, … Yang Gu

Identification of a binding protein for sesamin and characterization of its roles in plant growth

Article Open access 14 June 2019

Masayuki Tera, Tomotsugu Koyama, … Manabu Horikawa

Introduction

Sesame (Sesamum indicum) has been cultivated since antiquity and charred sesame was found in a stratum dated at between 3050 and 3500 B.C. at an Indus Valley Civilization site¹. Sesame seed oil is a rich source of unsaturated fatty acids, as well as lignans, which are phenylpropanoid-derived plant specialized metabolites chemically defined as monolignol dimers^2,3. (+)-Sesamin, (+)-sesamolin and (+)-sesaminol glucosides are the major lignans in sesame seed, and have attracted attention for their health-promoting activities. For example, (+)-sesamin is a phytoestrogen, a precursor of the mammalian lignans enterolactone and enterodiol, and exhibits protective activity against breast and prostate cancers⁴. The long-term consumption of (+)-sesaminol is believed to inhibit pathogenic extracellular β-amyloid aggregation associated with Alzheimer disease⁵. Moreover, sesamol, a sesame lignan-related metabolite, has been related to photoprotective effect in UV-B irradiated lymphocytes, and repression of melanin biosynthesis by reducing cAMP-dependent tyrosinase activity^6,7. Lignans are often observed in the seeds of oil crops, such as olive, safflower, and flaxseed, as well as sesame, and have been implicated to be potent defense phytochemicals against microbes and other plant species⁸. However, in sharp contrast to the wealth of knowledge on the biological activities of lignans in mammals, the physio-ecological roles of lignans in plants remain largely elusive.

The biosynthesis of sesame lignans up to (+)-sesamin is well characterized. The pathway branches out from a common cascade of phenylpropanoids by stereo-selective radical coupling of two molecules of a monolignol, coniferyl alcohol, guided by a dirigent protein⁹. This coupling reaction results in the formation of the (+)-enantiomer of pinoresinol, a central precursor of lignans. (+)-Pinoresinol is then converted to (+)-sesamin via sequential oxygenation resulting in the formation of two methylenedioxy bridges. This reaction is catalyzed by a single cytochrome P450 monooxygenase CYP81Q1, also known as piperitol/sesamin synthase¹⁰. In contrast, the terminal metabolism of sesame lignans, particularly (+)-sesamolin and (+)-sesaminol, remain enigmatic, with neither the type of oxygenation nor the putative substrate being well understood (Fig. 1a). This is primarily because (+)-sesamolin biosynthesis requires an atypical oxygen insertion between the furan and aromatic rings. This reaction has been rarely observed other than Justicia simplex and Phryma leptostachya^11,12. Through genetic, genomic and biochemical approaches, here we identify a P450 monooxygenase CYP92B14 as the enzyme that is responsible for the oxygenation of (+)-sesamin to form (+)-sesamolin and (+)-sesaminol. Furthermore, the labeling experiments using stable isotopes indicate that the oxygenation involves a novel reaction scheme designated as oxidative rearrangement of α-oxy-substituted aryl groups (ORA). The identification of CYP92B14 completes the biosynthesis of major lignans in sesame, and provides insights into yet to be characterized mechanisms of enzymatic oxygenation in specialized metabolites that possess heteroatoms at the α-position of alkyl-substituted aryl groups.

Results

Genetic analysis of sesamolin-deficient accession #4294

In order to identify enzyme genes responsible for the biosynthesis of (+)-sesamolin, recombinant inbred lines (RILs; F6 generation) of sesame were generated by crossing a (+)-sesamolin-deficient accession #4294 and a (+)-sesamolin-accumulating cultivar ITCFA2002 (Fig. 1b, Supplementary Table 1). The genetic analysis indicated that (+)-sesamolin was derived from (+)-sesamin, since (+)-sesamolin-deficient RILs accumulated increased amounts of (+)-sesamin compared to (+)-sesamolin-accumulating RILs (Fig. 1c, d). This notion was further supported by the higher level of (+)-sesamin in an another (+)-sesamolin-deficient cultivar Maruemon compared to its relative cultivars, Gomazou and Maruhime, both of which accumulate (+)-sesamolin¹³. The RILs were clearly grouped into two classes in a 1:1 ratio according to the level of (+)-sesamolin: of 160 lines, the relative content of (+)-sesamolin compared to total lignan was <5% in 81 lines but 15–45% in 79 lines (Fig. 1d, Supplementary Table 2). Moreover, the microsome fraction of the (+)-sesamolin-accumulating cultivar ‘Masekin’ exhibited biosynthetic activity of (+)-sesamolin from (+)-sesamin, but the activity in the fraction was abolished by heat inactivation prior to the assay (Fig. 1e). These collectively indicate that a single genetic locus encoding a microsomal enzyme is involved in the biosynthesis of (+)-sesamolin.

CYP92B14 is responsible for (+)-sesamolin biosynthesis

Restriction Site Associated DNA Sequence (RAD-Seq) analysis¹⁴ of the segregated RIL lines and genetic mapping using 566 single-nucleotide polymorphism (SNP) markers covering over 90% of the RILs indicated that a marker, #90036, on a genomic contig, linkage group 2 (LG2)¹⁵, was associated with (+)-sesamolin deficiency (Fig. 2a). There was a P450 gene cluster in the corresponding genomic region, where the open reading frame (ORF) of LOC105175169, designated as CYP92B14 by P450 monooxygenase nomenclature¹⁶, harbored a thymine insertion shared in #4294 and Maruemon, generating a mutant lacking the four C-terminal amino acids (Del4C) (Fig. 2c, Supplementary Fig. 1). The expression of CYP92B14 was increased during the mid-stage of seed development (Fig. 2b, Supplementary Figs. 2, 3). This profile coincided with that of sesamin synthase CYP81Q1 (Fig. 1a)¹⁰, and proceeded to the accumulation of (+)-sesamolin. In contrast, S. indicum cytochrome P450 oxidoreductase 1 (CPR1) was expressed throughout seed development. The expression level of CYP92B14 was considerably higher than that of any other CYP gene except LOC105175177 (Supplementary Fig. 3). CYP92B14 and four other P450s identified in LG2 were grouped into the CYP92 family, which is widely found in gymnosperms and angiosperms. These five P450s are phylogenetically close to PsDWF_like1¹⁷, which is involved in brassinosteroid biosynthesis, but distant from two phenylpropanoid-related P450s, coumarate 3-hydroxylase (C3H; CYP98A20) and CYP81Q1 (Fig. 2a, c, Supplementary Fig. 4).

Biochemical characterization of CYP92B14

Biochemical analysis showed that the microsome fraction from yeast cells expressing CYP92B14 and CPR1 converted (+)-sesamin to (+)-sesamolin (Fig. 3a). Furthermore, co-existence of CPR1 with CYP92B14 substantially enhanced the catalysis. Conversely, Del4C lost the ability to biosynthesize (+)-sesamolin (Fig. 3a). CO-difference spectrum analysis indicated that Del4C was expressed as an inactive form as shown by the absence of a peak at 450 nm (Fig. 3b), while its expression level in yeast was comparable to that of CYP92B14 (Fig. 3c, Supplementary Fig. 5). Moreover, Del4C in mature seeds of the (+)-sesamolin-deficient RIL (#119) was expressed at a level comparable to that of CYP92B14 in the (+)-sesamolin-accumulating RIL (#89) (Supplementary Fig. 6). On the other hand, introduction of the amino acid substitution P509S to CYP92B14 did not result in the loss of the catalytic activity (Supplementary Fig. 7). These data indicate that functional impairment originated from the lack of four amino acids in the C-terminus of CYP92B14, but not the lack of CYP92B14 expression, is responsible for (+)-sesamolin deficiency in #4294. Intriguingly, CYP92B14 also generated (+)-sesaminol from (+)-sesamin, whereas Del4C lacked such activity (Fig. 3a), showing that CYP92B14 is a multifunctional enzyme that co-generates (+)-sesamolin and (+)-sesaminol.

Effects of co-expression of CYP81Q1 on CYP92B14

Various plant P450 enzymes have been implicated to interact and form complexes called metabolon with enzymes that catalyze sequential reactions in a metabolic pathway¹⁸. To assess whether CYP81Q1 affects the catalytic activity of CYP92B14, CYP81Q1 was expressed in yeast cells together with CYP92B14 and CPR1, and was subjected to the bioassays. Yeast cells co-expressing CYP81Q1, CYP92B14, and CPR1 converted (+)-pinoresinol to (+)-sesaminol and (+)-sesamolin via (+)-sesamin, demonstrating sequential lignan biosynthesis by the distinct P450s (Fig. 4). Notably, when (+)-sesamin was supplied as a substrate, yeast cells harboring CYP81Q1, CYP92B14, and CPR1 produced increased amount of (+)-sesaminol and (+)-sesamolin compared to those expressing CYP92B14 and CPR1 but without CYP81Q1 (Fig. 4, Supplementary Fig. 7). Furthermore, while (+)-sesamin supplied as the substrate was detectable in yeast cells expressing CYP92B14 and CPR1, it was completely consumed in yeast cells expressing CYP92B14 and CYP81Q1 together with CPR1. On the other hand, CYP81Q1 alone did not react with (+)-sesamin, and CYP92B14 alone showed no apparent catalytic activity toward (+)-pinoresinol and (+)-piperitol. These data suggest catalytic cooperation between CYP81Q1 and CYP92B14 for biosynthesis of (+)-sesamolin and (+)-sesaminol.

CYP92B14 mediates unique oxygenation schemes

The oxygenation scheme by CYP92B14 was expected to be unique, since CYP92B14 co-generated twin products, (+)-sesaminol and (+)-sesamolin, from a single substrate (+)-sesamin. CYP92B14 incorporated oxygen from molecular oxygen, but not H₂O, for the oxygenation catalysis as with other P450s (Supplementary Fig. 8). NMR analysis of CYP92B14 assay products showed that the deuterium-labeling content in (+)-sesaminol and (+)-sesamolin was essentially identical to that in the substrate (+)-7,7′-²H₂-sesamin (80% ²H for each position) (Compound 1) (Fig. 5a). Alternatively, deuterium-labeling content in (+)-sesaminol was changed in the C3 and C6 positions (C3: 45% ²H, C6: 30% ²H) when (+)−2,2′-²H₂-sesamin (75% ²H for each position) (Compound 2) was used as a substrate (Fig. 5a). The deuterium-labeling content at the C2 or C2′ positions of (+)-sesamolin was unchanged from that of the substrate (+)-2,2′-²H₂-sesamin. These results show that, for the biosynthesis of (+)-sesamolin, CYP92B14 catalyzes C–O bond formation between the intermediate II and sesamol via intermediate I following the initial oxidation of (+)-sesamin at C1 position and the continuous cleavage of the furan and aromatic rings (Fig. 5b), which is comparable to that of P450-dependent C–C bond cleavage of p-hydroxybenzyl alcohol¹⁹. On the other hand, CYP92B14 biosynthesizes (+)-sesaminol by two separate pathways: (i) C–C bond formation between the intermediate II and sesamol as in the case of sesamolin biosynthesis, and (ii) direct oxygenation of the aromatic ring via intermediate III following the initial oxidation of (+)-sesamin at the C6 position (Fig. 5b). These oxygenation positions catalyzed by CYP92B14 for (+)-sesamolin and (+)-sesaminol biosynthesis were clearly distinct from that of (+)-episesaminone, which is tautomerically generated by oxygenation at the C7 position through hemiacetal intermediate^3,20. NMR analysis of CYP92B14 assay products using (+)−2,2′-²H₂-sesamin and (+)−7,7′-²H₂-sesamin also showed that CYP92B14-mediated catalysis yielded (+)-sesamolin through ORA, (+)-sesaminol through ORA and (+)-sesaminol through direct oxygenation in a molar ratio of 72:17:11, respectively, (Fig. 5b).

Discussion

CYP92B14 catalyzed the intricate reactions; a novel oxygenation designated as ORA and a well-characterized direct oxygenation of aromatic ring. ORA involved (i) the oxygenation of carbon atom on the aromatic ring system substituted with alkyl group, possibly via cation intermediate, (ii) the cleavage of the C–C bond between the aromatic and α-oxy-substituted alkyl groups, followed by (iii) the addition of the generated phenol adduct and the oxonium intermediate (Fig. 5b). Enzyme assays using deuterated (+)-sesamin clearly indicated that CYP92B14 catalyzed the oxidation of aromatic ring of (+)-sesamin. P450 monooxygenases in general operate the initial oxidation of a phenolic substrate through electrophilic attack of FeO complex to the aromatic ring, which generates a cation rather than radical intermediate²¹. Therefore, CYP92B14 likely generates cation intermediates I and III in the initial oxidation of (+)-sesamin. The preference of the generation of intermediate I over intermediate III, and that of C–O over C–C bonding in the addition of the phenol adduct to the oxonium intermediate (Fig. 5b) would largely reflect the structural constraints of the active site. Although plant specialized metabolites rarely harbor an insertion of an oxygen atom between aromatic and α-oxy-substituted alkyl group as in (+)-sesamolin¹¹, the initial oxygenation step of ORA might be widespread outside sesame lignans. For example, a quinoid glycoside forsythenside B²² from Forsythia suspensa, a pretocarpan hydroxycristacarpone²³ from Erythrina orientalis and a sesquiterpene hydroxycacalolide²⁴ from Ligularia virgaurea all possess 4-alkyl-4-hydroxy-2,5-cyclohexadienone partial structures generated by the oxidation of 4-alkylphenol compounds, respectively (Supplementary Fig. 9). Moreover, a characteristic hydroxyl group at the C1-position of the B-ring in protoapigenone and protogenkwanone in the fern genera Thelypteris and Pseudophegopteris likely to be generated by oxygenation analogous to that by CYP92B14 (Supplementary Fig. 9)^25,26. Furthermore, it is plausible that 5,7-dihydroxychromone accumulated in Magnifera foetida and various other plant species is derived from C–C bond cleavage of the flavanone naringenin (Supplementary Fig. 9)^{27,28,29,30,31,32}. Since no enzymes that catalyze these oxygenation reactions have been identified so far, CYP92B14 is the first example of enzymes that mediates the oxygenation of the carbon atom on the aromatic ring system substituted with alkyl group. It is of particular interest whether the putative P450 enzyme encoded by LOC105175177 exhibits any enzyme activity toward sesame lignans. However, our attempts to identify the activity of the P450 enzyme have not been successful so far, since we failed to express LOC105175177 in yeast (Supplementary Fig. 5). Future establishment of the expression system of LOC105175177 and its functional characterization in comparison to CYP92B14 may provide further insights into the molecular mechanism of ORA that is mediated by this type of P450 enzymes. ORA might also be applicable to other classes of specialized metabolites including isoquinoline alkaloids and lignin polymers, since these metabolites have α-oxy-substituted aromatic ring as a common structural feature (Supplementary Fig. 9). In particular, typical lignin polymers contain multiple putative sites for oxygen insertion by ORA, implicating that the use of enzymes with catalytic activity similar to CYP92B14 facilitates full decomposition of lignan units that are otherwise indigestible by other classes of lignin-degrading enzymes^33,34.

Although the C-terminal region of P450s is unlikely to be directly associated with the substrate-binding domain³⁵, the C-terminal region seems to be indispensable for the catalytic activity of P450. For example, an amino acid substitution P497R in Homo sapiens CYP27B1 leads to the functional impairment and is responsible for vitamin D-dependent rickets type I and cerebrotendinous xanthomatosis³⁶. On the other hand, in the case of CYP92B14 mutant Del4C, which lacks the last four residues in its C-terminus and contains P509S substitution because of the thymine insertion, is unable to catalyze the reaction (Fig. 2a, Supplementary Fig. 1), whereas P509S mutant harboring full-length sequence remains active (Supplementary Fig. 7). Although the molecular mechanism behind the loss of oxygenation activity of these P450s is unclear, these data corroborate the implicit functional importance of the C-terminal region of P450s³⁶.

Importantly, sesamol and (+)-samin, a hydrate of the oxonium intermediate, were generated when CYP92B14 was supplied with (+)-sesamin (Supplementary Fig. 10). Moreover, the supplementation of these two compounds clearly inhibited the biosynthetic activity of CYP92B14 to form (+)-sesamolin and (+)-sesaminol from (+)-sesamin (Supplementary Fig. 11). These results supported the notion that sesamol and intermediate II were potent reaction intermediates of (+)-sesamolin and (+)-sesaminol biosynthesis (Fig. 5b), and partly explained why the total amount of (+)-sesamolin and (+)-sesaminol produced was substantially smaller than the amount of (+)-sesamin consumed in the yeast bioassay (Fig. 4, Supplementary Fig. 10). The lack of sesamol and (+)-samin production by Del4C (Supplementary Fig. 10)³⁷ indicated that Del4C was unable to cleave the inert C–C bond between the furan and aromatic rings of (+)-sesamin.

The identification of CYP92B14 uncovered the final steps in the biosynthetic pathway of all major lignans in S. indicum, (+)-sesamin, (+)-sesaminol and (+)-sesamolin, being (+)-sesamin as a precursor for (+)-sesaminol and (+)-sesamolin (Supplementary Fig. 12). This was biochemically confirmed by the result that the expression of CYP81Q1, CYP92B14, and CPR1 in yeast cells was sufficient to produce (+)-sesaminol and (+)-sesamolin from (+)-pinoresinol (Fig. 4). (+)-Sesamolinol, although previously proposed to be a putative precursor for (+)-sesamolin biosynthesis³⁸, is unlikely to be directly related to the biosynthesis of (+)-sesamolin, since (+)-sesamolinol and (+)-piperitol were not accepted as substrates by CYP81Q1 and CYP92B14, respectively (Fig. 4)^10,39. However, there might be additional enzymes, other than CYP92B14, that are responsible for (+)-sesaminol biosynthesis in S. indicum, since sesamolin-deficient RILs accumulate (+)-sesaminol triglucoside (STG) in vivo to a level comparable to that of sesamolin-producing RILs, while Del4C does not biosynthesize (+)-sesaminol in vitro (Figs. 1d, 3a).

Co-generation of structurally different metabolites through ORA, as evidenced in this study, reveals the outstanding enzymatic plasticity of P450s to elaborate structurally diverged specialized metabolites for increased environmental fitness. CYP92B14 could have recently evolved following the establishment of (+)-sesamin biosynthesis in an ancestor of Sesamum spp., given that (i) (+)-sesamolin is a lineage-specific lignan in Sesamum spp.⁴⁰, (ii) (+)-sesamin is the specific substrate for CYP92B14, and (iii) CYP81Q1 and CYP92B14 are phylogenetically distinct P450s despite the high structural similarities between their substrates/products. The capability of CYP81Q1 to ameliorate the activity of CYP92B14 (Fig. 4) further substantiated the versatility of P450s in producing multiple specialized metabolites. Enzymes that were involved in primary metabolism (i.e., glycolysis, amino acid biosynthesis and TCA cycle), as well as various specialized metabolism in plants, in particular, have been implicated to form multiple enzyme complexes that carry out sequential enzymatic reactions^18,41. However, major effort has been made to detect physical association of enzymes that form metabolons, and only few examples biochemically rationalized the formation of such complexes^18,42,43,44. Therefore, our data suggesting that the activity of CYP92B14 is functionally associated with CYP81Q1 may provide a novel system to study structure-function relationship of enzymes that function cooperatively.

Consequently, our data not only complete the biosynthesis of major lignans in sesame, but provide tools for heterologous expression of valuable dietary lignans. Furthermore, our findings open a gate for novel chemical perspective with respect to oxygenation through ORA in various classes of specialized metabolites that possess heteroatoms at the α-position beyond sesame lignans (Supplementary Fig. 9), thus established a novel molecular basis for enlarging the structural diversity of specialized metabolites in nature.

Methods

Plant materials

Sesamum indicum cv. Masekin plants were cultivated in a field in Osaka, Japan. Immature seeds were collected along the developmental stage index as described previously¹⁰. S. indicum cv. Maruemon was a gift from National Agriculture and Food Research Organization (NARO). S. indicum ITCFA2002 was kindly provided by T. Amano (ITOCHU) and T. Kondo (Nippon Norin Seed). F1 plants and RILs from a cross between #4294 and ITCFA2002 were grown in a greenhouse at University of Toyama.

RAD-Seq and genetic analyses

Genomic DNA of RILs and their parental lines (#4294 and ITCFA2002) were isolated from young leaves using the cetyl trimethylammonium bromide (CTAB) method with some modifications. Briefly, a fine frozen tissue powder was washed three times with washing buffer (0.5 M HEPES (pH 8.0), 0.1% polyvinylpyrrolidone, 4% β-mercaptoethanol) and then subjected to DNA extraction by the CTAB method, but omitting the CsCl precipitation step. The genomic DNAs were adjusted to 50 ng/µl and used for RAD-Seq analysis as described previously⁴⁵. RIL SNP was genotyped by the derived Cleaved Amplified Polymorphic Sequence (dCAPS) method⁴⁶ using the primer sets 5′-GCCATCTTTGGTTTCTGGTG-3′ and 5′-TGGGACTGACTCAACGGC-3′ for the #90032 marker, and restriction enzyme Hae III. PCR was performed using TAKARA Ex Taq Hot Start Version (TAKARA) DNA polymerase under the following conditions: an initial cycle at 94 °C for 2 min, 35 cycles at 98 °C for 10 s, 60 °C for 30 s, 72°C for 30 s, and a final extension step at 72 °C for 2 min. Linkage analysis of RAD markers and the (+)-sesamolin content trait was performed using Antmap ver. 1.2 software⁴⁷.

RT-PCR

Total RNA was extracted from developing seeds using RNeasy Plant Mini Kit (Qiagen). After DNase I (TAKARA) treatment, cDNA was synthesized from oligo dT primer using PrimeScript RT reagent Kit (TAKARA). Reactions were conducted according to manufactures’ instructions. PCR was performed using primer sets (Supplementary Table 3) under the conditions described above. Primers for UBQ6 were designed as reported previously⁴⁸.

Phylogenetic analysis

Phylogenetic analysis of CYP92B14 and 12 other P450 monooxygenases from plants was conducted using the UPGMA method with MEGA7⁴⁹. The accession numbers of the genes subjected to phylogenetic analysis are as follows; SiCYP92B14, LC199944; SiCYP98A20, NP_001291339; SiCYP81Q1, BAE48234; SrCYP81Q2, BAE48235; SaCYP81Q3, BAE48236; PlCYP81Q38, BAP46307; GmCYP93C, SBU44858; AtCYP51G1, NP_172633; AtCYP98A3, OAP09214; PsDWF_like1, AAG44132; and GhDDWF1, ABA01477.

Chemicals

(+)-Pinoresinol and (+)-sesaminol (Nagara Science) were used without further purification. (+)-Sesamin (ChromaDex) was sequentially purified by normal, followed by reverse phase column chromatography. (+)-Sesamolin was isolated during the purification of (+)-sesamin. For the synthesis of (+)-samin, (+)-sesamolin was treated with 3N aqueous hydrogen chloride in dioxane at 45 °C for 1 h³⁷. For the synthesis of (+)-piperitol, (+)-sesamin was treated with diisobutyl aluminum hydride in toluene at 140 °C for 1 h³⁹. Sesamol and other chemical reagents, including solvents, were purchased from Tokyo Chemical Industry, Sigma-Aldrich or Nacalai Tesque.

Chemical synthesis of stable isotope-labeled sesamin

(+)-7,7′-²H₂-sesamin (1): (+)-Sesamin (270 mg, 0.762 mmol) was subjected to 4.0 kgf/cm² of hydrogen in the presence of Pd(en) (270 mg) in a mixture of 1,4-dioxane (4.2 ml) and deuterium oxide (1.8 ml) at 110 °C for 3 days⁵⁰. After filtration of the reaction mixture, the filtrate was purified by reverse phase column chromatography to provide deuterated 1 (153 mg, 0.429 mmol, 56%). The ²H content at the C7 and C7′ of deuterated (+)-sesamin was 80%, as estimated by comparison of the hydrogen intensity of H-7, 7′ and H-9a, 9′a in a ¹H NMR experiment.

1: mp 123–124 °C; [α]²⁵_D +67.7° (c 0.16, CHCl₃); ¹H NMR (800 MHz, CDCl₃) δ3.05 (2 H, m, H-8, 8′), 3.87 (2 H, dd, J = 2.4, 9.2 Hz, H-9a, 9′a), 4.23 (2 H, dd, J = 6.6, 9.2 Hz, H-9b, 9′b), 4.71 (2 H, d, J = 3.5 Hz, H-7, 7′), 5.95 (4 H, s, H-10, 10′), 6.78 (2 H, d, J = 8.0 Hz, H-5, 5′), 6.80 (2 H, br d, J = 8.0 Hz, H-6, 6′), 6.85 (2 H, br s, H-2, 2′); ¹³C NMR (200 MHz, CDCl₃) δ 54.2, 54.3 (C-8, 8′), 71.66, 71.67 (C-9, 9′), 85.4 (t, J = 22.0 Hz, C-7, 7′), 85.8 (C-7, 7′), 101.1 (C-10, 10′), 106.5 (C-2, 2′),108.2 (C-5, 5′), 119.3, 119.4 (C-6, 6′), 134.97, 135.03 (C-1, 1′), 147.0, 147.1 (C-4, 4′), 148.0(C-3, 3′). HRMS (ESI): [M+Na]⁺ calculated for C₂₀H₁₆D₂O₆Na: m/z 379.3549, found: 379.3542. NMR spectra of 1 are shown in Supplementary Figs. 13–15.

(+)-2,2′-²H₂-sesamin (2): Synthesis of (+)-2,2′-²H₂-sesamin was performed basically as reported previously⁵¹. To a solution of (+)-sesamin (354 mg, 1.0 mmol) in dry tetrahydrofuran (5 ml) was added n-BuLi (1.5 ml, 1.6 mol/L in toluene) at −78 °C under argon. After stirring for 30 min, methanol-d₄ (0.5 ml) was added, the solution was allowed to warm to room temperature, then the solution was partitioned between H₂O and ethyl acetate, the organic layer washed with saturated NaCl solution, dried (MgSO₄) and concentrated in vacuo to dryness. The resulting residue was purified by reverse phase column chromatography to provide deuterated 2 (185 mg, 0.519 mmol, 52%). The ²H content at the C2 and C2′ of deuterated (+)-sesamin was 75%, as estimated by comparison of the hydrogen intensity of H-2, 2′ and H-9a, 9′a in a ¹H NMR experiment.

2: mp 123–124 °C; [α]²⁵_D +68.6° (c 0.18, CHCl₃); ¹H NMR (800 MHz, CDCl₃) δ3.05 (2 H, m, H-8, 8′), 3.87 (2 H, dd, J = 3.7, 9.2 Hz, H-9a, 9′a), 4.23 (2 H, dd, J = 6.7, 9.2 Hz, H-9b, 9′b), 4.71 (2 H, d, J = 4.2 Hz, H-7, 7′), 5.95 (4 H, s, H-10, 10′), 6.78 (2 H, d, J = 8.0 Hz, H-5, 5′), 6.80 (2 H, br d, J = 8.0 Hz, H-6, 6′), 6.85 (2 H, br d, J = 1.6 Hz, H-2, 2′); ¹³C NMR (200 MHz, CDCl₃) δ54.3 (C-8, 8′), 71.6 (C-9, 9′), 85.70, 85.73 (C-7, 7′), 101.0 (C-10, 10′), 106.2 (t, J = 24.8 Hz, C-2, 2′), 106.4 (C-2, 2′), 108.1 (C-5, 5′), 119.3 (C-6, 6′), 134.9, 135.0 (C-1, 1′), 147.05, 147.06 (C-4, 4′), 147.85, 147.91 (C-3, 3′); HRMS (ESI): [M+Na]⁺ calculated for C₂₀H₁₆D₂O₆Na: m/z 379.3549, found: 379.3540. NMR spectra of 2 are shown in Supplementary Figs. 16–18.

LC–MS analysis of stable isotope labeled compounds

High-resolution mass spectra of all products including deuterated or ¹⁸O-labeled compounds were obtained using an ion-trap time-of-flight mass spectrometer (Shimadzu LCMS-IT-TOF). A YMC-Triart C18 (100 × 2.1 mm, 3.0 μm) column was used for separation of enzymatic products derived from (+)-sesamin or deuterated (+)-sesamin. The mobile phase consisted of 0.1% formic acid in MilliQ water (A) and 0.1% formic acid in methanol (B) and used for a linear gradient elution (0–7–10–10.01–15 min, 30–90–90–30–30%B). Positive and negative ions were measured simultaneously. The parameters were as follows: nebulizer gas, 1.5 L/min; drying gas pressure, 190 kPa; CDL temperature, 200 °C; block heater temperature, 200 °C; detector, 1.64 kV; and interface, 4.5 kV (positive mode) and −3.5 kV (negative mode).

NMR analysis of stable isotope labeled compounds

All NMR spectra of all products including deuterated compounds were acquired on a Bruker AVANCE III HD 800 spectrometer equipped with a 5-mm TCI cryogenic probe and Z-axis gradient (Bruker Biospin). All spectra were measured at 25 ºC, using 5 mm NMR tubes. Standard Bruker pulse sequences were employed. The data analyses were carried out with Bruker TopSpin 3.2 software (Bruker Biospin). The acquisition parameters were as follows: ¹H NMR: number of data points 64 K; spectral width 16025 Hz; relaxation delay 10 s; number of scans 64; receiver gain 3.2; ¹³C NMR: number of data points 64 K; spectral width 48077 Hz; relaxation delay 2 s; number of scans 1024; receiver gain 203; ²H NMR: number of data points 64 K; spectral width 4092 Hz; relaxation delay 1 s; number of scans 1024; receiver gain 128. Chemical shift was reported in parts per million (p.p.m.) relative to the peaks from chloroform-d (CDCl₃, δ_H 7.26 p.p.m., δ_C 77.0 p.p.m.). The data for ¹H NMR are reported as follows: chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet), integration and coupling constants.

RNA-seq

Total RNA was extracted from six developmental stages of seeds and germinating seeds (3 days after imbibition) using RNeasy Plant Kit (Qiagen). The developmental stages of sesame seeds were described previously¹⁰. The RNA samples were treated with DNase Set (Qiagen) to remove contaminating genomic DNA. The quality of each RNA sample was evaluated using BioAnalyzer (Agilent Technologies) with RNA6000 Nano Chip. A 1 mg aliquot of total RNA from each sample was used to construct cDNA libraries using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina) according to the manufacturer’s instructions. The resulting cDNA library was validated using BioAnalyzer with DNA1000 Chip and quantified using Cycleave PCR Quantification Kit (TAKARA). Paired-end sequencing using 2 × 101 cycles was performed using HiSeq 1500 sequencing system (Illumina) in the high output mode.

Total reads were extracted using CASAVA v1.8.2 (Illumina), then PCR duplicates, adaptor sequences, and low quality reads were removed from the extracted reads as follows. Briefly, if the first 10 bases of two reads were identical and the entire reads showed >90% similarity, the reads were considered to be PCR duplicates. Base calling from the 5′ to the 3′ end was stopped when the frequency of accurately called bases dropped to 0.5. The remaining reads were assembled using Trinity (25 February 2013 release) by normalization with maximum coverage to 30. For each sample, the Fragments per Kilobase Megareads (FPKM) values were calculated for contigs containing cDNA sequences responsible for CYP92B14, CYP81Q1, and CPR1 using Bowtie.

qPCR analysis

qPCR analysis was performed as described previously⁵². Briefly, 18S rRNA was used as a reference gene for normalization and presented as expression ratios relative to control via the ΔΔCt method. Oligonucleotides used for the analysis are listed in Supplementary Table 3.

Enzyme assays using sesame microsome fractions

Fresh immature seeds (1 g) in Stage 4 were frozen in liquid nitrogen and homogenized to a fine powder under liquid nitrogen cooling using TissueLyzer II (Qiagen), then resuspended in 2 ml of extraction buffer I containing 100 mM Tris-Cl (pH 8.0), 0.1% β-mercaptoethanol, 1× Complete EDTA-free protease inhibitor (Roche) and 20% glycerol. The homogenate was desalted using a MicroSpin HR S-400 column (GE Healthcare) and centrifuged at 21,000×g for 10 min at 4 °C, then the supernatant was ultracentrifuged at 70,000×g for 1 h at 4 °C. The pellet was resuspended in 200 µl of Extraction buffer I. The microsome fraction (10 µl) was mixed with 100 µM (+)-sesamin and 1 mM NADPH in a final volume of 50 µl and incubated for 30 min at 37 °C. Heat inactivation was conducted by incubating the microsome fraction at 96 °C for 5 min prior to the enzyme assay. The reaction was terminated by the addition of an equal volume of acetonitrile, the reaction mixture was filtered through a Millex-LH syringe filter (Merck Millipore), then analyzed either by using an HPLC equipped with a photodiode array detector (Waters)⁵³ or an LC–MS equipped with a fluorescent detector (Shimadzu), unless otherwise stated.

Plasmid construction and yeast transformation

cDNAs of CYP92B14, Del4C, CPR1, and CYP81Q1 were amplified with cDNAs derived from sesame seeds at developmental stage 4 as the PCR templates and specific primer sets, described in Supplementary Table 3. Amplified fragments were inserted into the yeast expression vectors pYE22m, pDEST52 (Invitrogen), and pJHXSBP-His using standard procedures. The plasmid list and vector information are shown in Supplementary Table 3. Individual genes cloned in each vector were verified by DNA sequencing of both strands. Yeast INVsc strain (Invitrogen) was transformed using a conventional method with yeast expression vectors carrying Sesamum genes. The transformed yeast colonies were isolated on SD agar plates without selection compounds for 2 days at 30 °C.

Enzyme assays using yeast microsome fractions

Yeast cell lines expressing CYP92B14 and CPR1, Del4C and CPR1, CYP92B14 alone or CPR1 alone were precultured in 50 ml SD medium lacking respective amino acids overnight at 120 r.p.m. 30 °C. The saturated culture was transferred to 950 ml SD liquid medium without respective amino acids and cultured at 120 r.p.m. 30 °C until the OD₆₀₀ reached 2.0. The cells were harvested by centrifugation at 10,000×g for 15 min at 4 °C. The pellet was rinsed once and resuspended in 10 ml of Extraction buffer I, followed by sonication at 4 °C. The homogenate was centrifuged at 21,000×g for 10 min at 4 °C, then the supernatant was ultracentrifuged at 70,000×g for 1 h at 4 °C. The pellet was resuspended in 600 µl of extraction buffer I and subjected to enzyme assays. The microsome fraction (50 µl) was mixed with 100 µM (+)-sesamin and 1 mM NADPH in a final volume of 100 µl and incubated for 60 min at 37 °C. The reaction was terminated by the addition of an equal volume of acetonitrile, and the mixture was filtered through a Millex-LH syringe filter (Merck Millipore), then subjected to HPLC analysis.

Bioassay experiments in yeast cells

For 1 ml-scale experiments, single colonies of yeast strains expressing the ORF sets CYP92B14+CPR1 or Del4C+CPR1 were cultured in 3 ml synthetic defined liquid medium overnight at 30 °C with rotary shaking at 120 r.p.m. The stationary phase culture (50 µl) was transferred to 1 ml of fresh medium in 24-well plates supplemented with 100 µM of the lignan being tested as a substrate in the experiment. The cultures were further incubated for 24 h unless otherwise stated at 30 °C with rotary shaking at 120 r.p.m. The efficiency of lignan extraction from the culture by the addition of an equal volume of acetonitrile was verified (Supplementary Fig. 19). Briefly, the cells were collected together with the medium and disrupted by sonication. The homogenate (50 µl) was mixed with 50 µl acetonitrile and centrifuged at 21,000×g for 10 min. The supernatant was filtered through a Millex-LH syringe filter and subjected to LC–MS analysis. The identity of the reaction products was confirmed by comparing the retention time and parent mass with authentic lignan standards.

For the ²H-labeling studies, yeast cells carrying CYP92B14+CPR1 were cultured in 600 ml of liquid medium containing 200 µM of ²H-labeled sesamin for 48 h at 30 °C with rotary shaking at 120 r.p.m., then extracted twice with an equal volume of ethyl acetate. The organic layer was dehydrated with magnesium sulfate, dried in vacuo to dryness, and reconstituted with 400 µl of 50% acetonitrile. The extract was fractionated by HPLC, and the fractions containing enzyme reaction products were subjected to MS and NMR analyses. NMR spectra of enzyme reaction products are shown in Supplementary Figs. 20–23.

For the ¹⁸O-labeling studies, either yeast medium prepared with ¹⁸O-labeled H₂O (Taiyo Nippon Sanso), or yeast medium prepared with non-labeled H₂O and aerated with ¹⁸O-labeled O₂ (GL Science), was used for the yeast bioassay experiments. Briefly, the cells were cultured in 1 ml of ¹⁸O-labeled medium containing 100 µM sesamin in 24-well plates for 48 h at 30 °C with rotary shaking at 120 r.p.m. Alternatively, 50 µl of stationary phase liquid cultures of yeast cells were sub-cultured in 1 ml of non-labeled liquid medium bubbled in advance with ¹⁸O-labeled O₂ at a rate of 1 ml/min for 10 min. The air space in the test tubes was replaced with ¹⁸O-labeled O₂, then the tubes were sealed tightly and the yeast cells were cultured for 4 h at 30 °C with rotary shaking at 120 r.p.m. The reactions were terminated by the addition of an equal volume of acetonitrile, filtered through a Millex-LH filter, and analyzed by LC–MS.

CO-difference spectroscopy

Yeast cells expressing the ORF sets CYP92B14+CPR1 or Del4C+CPR1 were cultured in two three liter flasks containing one liter medium to an OD₆₀₀ of 2.0. The cells were collected by centrifugation at 10,000×g for 15 min at 4 °C and rinsed once with 50 ml of distilled water. The cells were resuspended in extraction buffer I, disrupted by sonication on ice, then the homogenate was centrifuged at 10,000×g for 15 min at 4 °C, followed by ultracentrifugation at 75,000×g for 1 h at 4 °C. The pellet was resuspended in 600 µl of extraction buffer. CO-difference spectroscopy was conducted basically as described previously⁵⁴. Briefly, the absorbance spectrum (400–500 nm) of the obtained microsome fraction was measured, then the microsome fraction was reduced by the addition of sodium dithionite and the spectrum of the reduced form was measured. The microsome fraction was then saturated with CO by bubbling CO for 1 min and the spectrum of the CO-bound form of P450 monooxygenase was measured.

Immunoblot analysis

Microsomal proteins of yeast cells expressing CPR1 with either CYP92B14 or Del4C were separated by SDS-PAGE and subjected to immunoblot analysis. Briefly, 20 µg of microsomal proteins per lane was separated by SDS-PAGE using a 5–20% acrylamide gradient gel and blotted onto a Hybond-P VDF membrane (GE Healthcare). Primary antibody reactions used 500× diluted rabbit polyclonal antibodies raised against a 1:1 mixture of the oligopeptides NH₂-C+WRQARKIYLSEVF-COOH and NH₂-C+QFLRLHDKVFASRP-COOH (Eurofins Genomics), both of which are perfectly conserved among all the P450 monooxygenases analyzed in the immunoblot analysis. Secondary antibody reactions used 5000× diluted rabbit IgG-HRP (GE Healthcare). Chemiluminescent detection was performed using ECL Select (GE Healthcare) as a substrate with Amersham Imager 600 (GE Healthcare) (Supplementary Fig. 5).

Data availability

The authors declare that fastq files for RNASeq data have been deposited in the NCBI SRA repository under Accession ID PRJNA350858. Sequences for CYP92B14 and CPR1 have been deposited in GenBank under accession codes LC199944 and LC209223, respectively. The authors declare that all other data supporting the findings of this study are available within the manuscript and its Supplementary Files or are available from the corresponding author upon request.

Change history

25 May 2018
The original version of of the Supplementary Information associated with this Article inadvertently omitted oligonucleotide primer sequences from Supplementary Table 3 and Supplementary Methods describing the molecular cloning of CYP92B14, CPR1 and CYP81Q cDNA fragments. The HTML has been updated to include a corrected version of the Supplementary Information.

References

Bedigian, D. & Harlan, J. R. Evidence for cultivation of sesame in the ancient world. Econ. Bot. 40, 137–154 (1986).
Article Google Scholar
Umezawa, T. Diversity in lignan biosynthesis. Phytochem. Rev. 2, 371–390 (2003).
Article CAS Google Scholar
Davin, L. B. & Lewis, N. G. An historical perspective on lignan biosynthesis: Monolignol, allylphenol and hydroxycinnamic acid coupling and downstream metabolism. Phytochem. Rev. 2, 257–288 (2003).
Article CAS Google Scholar
Liu, Z., Saarinen, N. M. & Thompson, L. U. Sesamin is one of the major precursors of mammalian lignans in sesame seed (Sesamum indicum) as observed in vitro and in rats. J. Nutr. 136, 906–912 (2006).
Article PubMed CAS Google Scholar
Katayama, S. et al. Effects of sesaminol feeding on brain Aβ accumulation in a senescence-accelerated mouse-prone 8. J. Agric. Food Chem. 64, 4908–4913 (2016).
Article PubMed CAS Google Scholar
Baek, S. & Lee, S. Sesamol decreases melanin biosynthesis in melanocyte cells and zebrafish: possible involvement of MITF via the intracellular cAMP and p38/JNK signalling pathways. Exp. Dermatol. 24, 761–766 (2015).
Article PubMed PubMed Central CAS Google Scholar
Prasad, N. R., Mahesh, T., Menon, V. P., Jeevanram, R. K. & Pugalendi, K. V. Photoprotective effect of sesamol on UVB-radiation induced oxidative stress in human blood lymphocytes in vitro. Environ. Toxicol. Pharmacol. 20, 1–5 (2005).
Article PubMed CAS Google Scholar
Yamauchi, S., Ichikawa, H., Nishiwaki, H. & Shuto, Y. Evaluation of plant growth regulatory activity of furofuran lignan bearing a 7,9’:7’,9-diepoxy structure using optically pure (+)- and (-)-enantiomers. J. Agric. Food Chem. 63, 5224–5228 (2015).
Article PubMed CAS Google Scholar
Davin, L. B. et al. Stereoselective bimolecular phenoxy radical coupling by an auxiliary (dirigent) protein without an active center. Science 275, 362–366 (1997).
Article PubMed CAS Google Scholar
Ono, E. et al. Formation of two methylenedioxy bridges by a Sesamum CYP81Q protein yielding a furofuran lignan, (+)-sesamin. Proc. Natl Acad. Sci. USA 103, 10116–10121 (2006).
Article ADS PubMed PubMed Central CAS Google Scholar
Ghosal, H., Banerjee, S. & Srivastava, S. Simplexolin, A new lignan from Justicia simplex. Phytochemistry 18, 503–505 (1979).
Article CAS Google Scholar
Zhu, H., Zhao, D., Deng, J. & Zhang, Y. Two new lignans from Phryma leptostachya L. Helv. Chem. Acta 96, 1392–1396 (2013).
Article CAS Google Scholar
Ide, T. et al. Comparative effects of sesame seeds differing in lignan contents and composition on fatty acid oxidation in rat liver. J. Oleo. Sci. 64, 211–222 (2015).
Article PubMed CAS Google Scholar
Etter, P. D., Bassham, S., Hohenlohe, P. A., Johnson, E. A. & Cresko, W. A. SNP discovery and genotyping for evolutionary genetics using RAD sequencing. Methods Mol. Biol. 772, 157–178 (2011).
Article PubMed PubMed Central CAS Google Scholar
Wang, L. et al. Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis. Genome Biol. 15, R39 (2014).
Article PubMed PubMed Central Google Scholar
Nelson, D. R. The cytochrome p450 homepage. Hum. Genom. 4, 59–65 (2009).
CAS Google Scholar
Kang, J. G. et al. Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth. Cell 105, 625–636 (2001).
Article PubMed CAS Google Scholar
Singleton, C., Howard, T. P. & Smirnoff, N. Synthetic metabolons for metabolic engineering. J. Exp. Bot. 65, 1947–1954 (2014).
Article PubMed CAS Google Scholar
Ohe, T., Mashino, T. & Hirobe, M. Substituent elimination from p-substituted phenols by cytochrome P450 ipso-substitution by the oxygen atom of the active species. Drug Metab. Dispos. 25, 1–5 (1997).
Google Scholar
Grougnet, R., Magiatis, P., Mitakou, S. & Skaltsounis, A. in Sesame: the genus Sesamum (ed. Bedigian, D.) 93–110 (CRC Press, Florida, 2006).
Tomberg, A., Pottel, J., Liu, Z., Labute, P. & Moitessier, N. Understanding P450-mediated bio-transformations into epoxide and phenolicmetabolites. Angew. Chemie-Int. Ed. 54, 13743–13747 (2015).
Article CAS Google Scholar
Ming, D. S., Yu, D. Q. & Yu, S. S. New quinoid glycosides from Forsythia suspensa. J. Nat. Prod. 61, 377–379 (1998).
Article PubMed CAS Google Scholar
Tanaka, H., Tanaka, T. & Etoh, H. A pterocarpan from Erythrina orientali. Phytochemistry 42, 1473–1475 (1996).
Article CAS Google Scholar
Saito, Y. et al. Chemical and genetic diversity of Ligularia virgaurea collected in northern Sichuan and adjacent areas of China. Isolation of 13 new compounds. Tetrahedron 68, 10011–10029 (2012).
Article CAS Google Scholar
Wada, H., Fujita, H., Murakami, T., Saiki, Y. & Chen, C.-M. Chemical and chemotaxonomical studies of ferns. LXXIII. New flavonoids with modified B-Ring from the genus Pseudophegopteris (Thelypteridaceae). Chem. Pharm. Bull. 35, 4757–4762 (1987).
Article CAS Google Scholar
Lin, A. S., Chang, F. R., Wu, C. C., Liaw, C. C. & Wu, Y. C. New cytotoxic flavonoids from Thelypteris torresiana. Planta. Med. 71, 867–870 (2005).
Article PubMed CAS Google Scholar
Liao, S. G. et al. Identification and characterisation of phenolics in polygonum capitatum by ultrahigh-performance liquid chromatography with photodiode array detection and tandem mass spectrometry. Phytochem. Anal. 24, 556–568 (2013).
Article PubMed CAS Google Scholar
Tang, Y. et al. Potential therapeutic agents for circulatory diseases from Bauhinia glauca Benth.subsp. pernervosa. (Da Ye Guan Men). Bioorg. Med. Chem. Lett. 25, 3217–3220 (2015).
Article PubMed CAS Google Scholar
Jo, Y. H. et al. Benzylated and prenylated flavonoids from the root barks of Cudrania tricuspidata with pancreatic lipase inhibitory activity. Bioorganic Med. Chem. Lett. 25, 3455–3457 (2015).
Article CAS Google Scholar
Panthong, K. et al. Two new triterpenes and a new coumaroyl glucoside from the twigs of Mangifera foetida Lour. Phytochem. Lett. 11, 43–48 (2015).
Article CAS Google Scholar
Tanaka, T., Watarumi, S., Matsuo, Y., Kamei, M. & Kouno, I. Production of theasinensins A and D, epigallocatechin gallate dimers of black tea, by oxidation-reduction dismutation of dehydrotheasinensin A. Tetrahedron 59, 7939–7947 (2003).
Article CAS Google Scholar
Leu, Y. L., Kuo, S. M., Hwang, T. L. & Chiu, S. T. The inhibition of superoxide anion generation by neutrophils from Viscum articulactum. Chem. Pharm. Bull. 52, 858–860 (2004).
Article PubMed CAS Google Scholar
Vanholme, R., Demedts, B., Morreel, K., Ralph, J. & Boerjan, W. Lignin biosynthesis and structure. Plant Physiol. 153, 895–905 (2010).
Article PubMed PubMed Central CAS Google Scholar
Bugg, T. D. H., Ahmad, M., Hardiman, E. M. & Singh, R. The emerging role for bacteria in lignin degradation and bio-product formation. Curr. Opin. Biotechnol. 22, 394–400 (2011).
Article PubMed CAS Google Scholar
Johnson, E. F. & Stout, C. D. Structural diversity of eukaryotic membrane cytochrome P450s. J. Biol. Chem. 288, 17082–17092 (2013).
Article PubMed PubMed Central CAS Google Scholar
Sawada, N., Sakaki, T., Kitanaka, S., Kato, S. & Inouye, K. Structure-function analysis of CYP27B1 and CYP27A1. Eur. J. Biochem. 268, 6607–6615 (2001).
Article PubMed CAS Google Scholar
Fukuda, Y., Isobe, M., Nagata, M., Osawa, T. & Namiki, M. Acidic transformation of sesamolin, the sesame-oil constituent, into an antioxidant bisepoxylignan, sesaminol. Heterocycles 24, 923–926 (1986).
Article CAS Google Scholar
Marchand, P. A., Zajicek, J. & Lewis, N. G. Oxygen insertion in Sesamum indicum furanofuran lignans. Diastereoselective syntheses of enzyme substrate analogues. Can. J. Chem. 75, 840–849 (1997).
Article PubMed CAS Google Scholar
Noguchi, A. et al. Mode-of-action and evolution of methylenedioxy bridge forming P450s in plant specialized metabolism. Plant Biotechnol. 31, 493–503 (2014).
Article CAS Google Scholar
Bedigian, D., Seigler, D. S. & Harlan, J. R. Sesamin, sesamolin and the origin of sesame. Biochem. Syst. Ecol. 13, 133–139 (1985).
Article CAS Google Scholar
Laursen, T., Møller, B. L. & Bassard, J. E. Plasticity of specialized metabolism as mediated by dynamic metabolons. Trends Plant Sci 20, 20–32 (2015).
Article PubMed CAS Google Scholar
Bulutoglu, B. et al Direct evidence for Metabolon Formation and Substrate Channeling in Recombinant TCA Cycle Enzymes. ACS Chem. Biol. 11, 2847–2853 (2016).
Article PubMed CAS Google Scholar
Laursen, T. et al. Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Science 354, 890–893 (2016).
Article ADS PubMed CAS Google Scholar
Waki, T. et al. Identification of protein-protein interactions of isoflavonoid biosynthetic enzymes with 2-hydroxyisoflavanone synthase in soybean (Glycine max (L.) Merr.). Biochem. Biophys. Res. Commun. 469, 546–551 (2016).
Article PubMed CAS Google Scholar
Kawamura, K. et al. Genetic distance of inbred lines of Chinese cabbage and its relationship to heterosis. Plant Gene 5, 1–7 (2016).
Article ADS CAS Google Scholar
Neff, M. M., Neff, J. D., Chory, J. & Pepper, A. E. dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications inArabidopsis thaliana genetics. Plant J. 14, 387 (1998).
Article PubMed CAS Google Scholar
Iwata, H. & Ninomiya, S. AntMap: constructing genetic linkage maps using an ant colony optimization algorithm. Breed Sci. 56, 371–377 (2006).
Article Google Scholar
Wei, L., Miao, H., Zhao, R., Han, X. & Zhang, T. Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR. Planta 237, 873–889 (2013).
Article PubMed CAS Google Scholar
Kumar, S., Stecher, G. & Tamura, K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33, 1870–1874 (2016).
Article PubMed CAS Google Scholar
Kurita, T. et al. Efficient and convenient heterogeneous palladium-catalyzed regioselective deuteration at the benzylic position. Chem.-A Eur. J. 14, 664–673 (2008).
Article CAS Google Scholar
Kato, M. J., Chu, A., Davin, L. B. & Lewis, N. G. Biosynthesis of antioxidant lignans in Sesamum indicum seeds. Phytochemistry 47, 583–591 (1998).
Article CAS Google Scholar
Noguchi, A. et al. Sequential glucosylation of a furofuran lignan, (+)-sesaminol, by Sesamum indicum UGT71A9 and UGT94D1 glucosyltransferases. Plant J. 54, 415–427 (2008).
Article PubMed CAS Google Scholar
Kim, H. J. et al. Metabolic engineering of lignan biosynthesis in forsythia cell culture. Plant Cell Physiol. 50, 2200–2209 (2009).
Article PubMed CAS Google Scholar
Omura, T. & Sato, R. The Carbon Monoxide-biding pigment of Liver Microsomes. J. Biol. Chem. 239, 2370–2378 (1964).
PubMed CAS Google Scholar

Download references

Acknowledgments

We thank T. Amano and T. Kondo for ITCFA2002 seeds, and NARO for Maruemon seeds. We are also grateful to K. Namba, T. Sakaki, T. Umezawa, T. Waki, S. Takahashi, T. Nakayama, H. Takagi, H. Satake, K. Shimamoto, S. Nakanishi, H. Hatanaka, M. Fukuchi-Mizutani, M. Nakai, Y. Ono, and K. Yamada for fruitful discussion. This research was supported partly by JST PRESTO JP16H01473 (A.J.N.) and Grants-in-Aid for Scientific Research (C) JP25450003 (M.P.Y.).

Author information

Jun Murata, Eiichiro Ono, and Seigo Yoroizuka contributed equally to this work.

Authors and Affiliations

Bioorganic Research Institute, Suntory Foundation for Life Sciences (SUNBOR), 8-1-1 Seikadai, Seika, Soraku, Kyoto, 619-0284, Japan
Jun Murata, Akira Shiraishi, Shoko Mori, Masayuki Tera, Toshiaki Azuma & Manabu Horikawa
Research Institute, Suntory Global Innovation Center Ltd (SIC), 8-1-1 Seikadai, Seika, Soraku, Kyoto, 619-0284, Japan
Eiichiro Ono & Hiromi Toyonaga
Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan
Seigo Yoroizuka, Tatsuya Wakasugi & Masayuki P. Yamamoto
Faculty of Agriculture, Ryukoku University, 1-5 Yokotani, Seta Oe, Otsu, Shiga, 520-2914, Japan
Atsushi J. Nagano
JST CREST, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan
Atsushi J. Nagano
Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan
Masaru Nakayasu & Masaharu Mizutani

Authors

Jun Murata
View author publications
You can also search for this author in PubMed Google Scholar
Eiichiro Ono
View author publications
You can also search for this author in PubMed Google Scholar
Seigo Yoroizuka
View author publications
You can also search for this author in PubMed Google Scholar
Hiromi Toyonaga
View author publications
You can also search for this author in PubMed Google Scholar
Akira Shiraishi
View author publications
You can also search for this author in PubMed Google Scholar
Shoko Mori
View author publications
You can also search for this author in PubMed Google Scholar
Masayuki Tera
View author publications
You can also search for this author in PubMed Google Scholar
Toshiaki Azuma
View author publications
You can also search for this author in PubMed Google Scholar
Atsushi J. Nagano
View author publications
You can also search for this author in PubMed Google Scholar
Masaru Nakayasu
View author publications
You can also search for this author in PubMed Google Scholar
Masaharu Mizutani
View author publications
You can also search for this author in PubMed Google Scholar
Tatsuya Wakasugi
View author publications
You can also search for this author in PubMed Google Scholar
Masayuki P. Yamamoto
View author publications
You can also search for this author in PubMed Google Scholar
Manabu Horikawa
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

M.P.Y. and M.H. designed the research, S.Y., T.W., and M.P.Y. performed genetic analyses, E.O. and H.T. cloned CYP92B14, A.J.N. conducted RAD-Seq analyses, E.O. and A.S. analyzed RNA-Seq data, J.M. and M.H. performed biochemical analyses, M.N. and M.M. performed CO-difference spectra analysis, M.H., M.T., and T.A. conducted chemical syntheses, M.H. and S.M. performed NMR analyses, and J.M., E.O., M.P.Y., and M.H. wrote the manuscript. J.M., E.O., and S.Y. contributed equally to this work.

Corresponding authors

Correspondence to Masayuki P. Yamamoto or Manabu Horikawa.

Ethics declarations

Competing interests

E.O. and H.T. are employees of Suntory Global Innovation Center, Ltd. Suntory Foundation for Life Sciences is a non-profit organization. The corresponding authors had full access to all the data in the study and had final responsibility for the decision to submit for publication. All other authors declare no competing financial interests.

Additional information

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Supplementary Information

Peer Review File

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Murata, J., Ono, E., Yoroizuka, S. et al. Oxidative rearrangement of (+)-sesamin by CYP92B14 co-generates twin dietary lignans in sesame. Nat Commun 8, 2155 (2017). https://doi.org/10.1038/s41467-017-02053-7

Download citation

Received: 28 March 2017
Accepted: 02 November 2017
Published: 18 December 2017
DOI: https://doi.org/10.1038/s41467-017-02053-7

This article is cited by

Omics technologies towards sesame improvement: a review
- Micheale Yifter Weldemichael
- Hailay Mehari Gebremedhn
Molecular Biology Reports (2023)
Transgenic Forsythia plants expressing sesame cytochrome P450 produce beneficial lignans
- Tomotsugu Koyama
- Erika Matsumoto
- Honoo Satake
Scientific Reports (2022)
Inter-specific hybrid sesame with high lignan content in oil reveals increased expression of sesamin synthase gene
- Debabrata Dutta
- Ranjana Prasad
- Gaurab Gangopadhyay
The Nucleus (2022)
Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue
- Huihui Zhu
- Honglian Ai
- Liang Chen
BMC Genomics (2020)
Identification of a binding protein for sesamin and characterization of its roles in plant growth
- Masayuki Tera
- Tomotsugu Koyama
- Manabu Horikawa
Scientific Reports (2019)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.