Fig. 4 | Nature Communications

Fig. 4

From: Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Fig. 4

DNA-PAINT imaging of distinct types of biomolecular targets at single optical sections. a Three-color immunostaining of fixed HeLa cells for histone variant MacroH2A.1 (red), Lamin B (green), and nucleophosmin (blue) at 3.6 µm height from the glass surface. b DNA FISH in EY.T4 mouse embryonic fibroblasts for the major satellite (magenta) and immunostaining for Lamin A (green) at 4.6 µm height from the glass surface. c Three-color DNA FISH against the major satellite (magenta), minor satellite (white), and telomere regions (green) in mouse embryonic fibroblasts. d Two-color DNA FISH against single-copy targets at Xq28 in human female WI-38 fetal lung fibroblast cells. e RNA FISH against the Xist RNA in human female IMR-90 fetal lung fibroblast cells. f Single-molecule RNA FISH against the CBX5 mRNA in HeLa cells. Scale bars, 2 µm (ab, and f), 1 µm (ce)

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