Fig. 3 | Nature Communications

Fig. 3

From: Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Fig. 3

Multiplexed 3D-Exchange-PAINT optical section inside a cell. a 2D projection of a 500-nm thick SDC slice ~3 µm inside a fixed HeLa cell. Two-target Exchange-PAINT targeting TOM20 (red) and HSP60 (green). Top left: Schematic drawing of the cell along with the detection volume (red). The 500-nm thick detection volume is split up into smaller slices (100 nm thick, see zoom-in) using astigmatism-based 3D super-localization. b Comparison of diffraction-limited (DL) and DNA-PAINT image (SR) of the highlighted area in a. c 3D super-resolved z-slice (0–100 nm, see height indicator on the top right) of the highlighted area in a. The arrows highlight areas that change appearance throughout the whole 500 nm slice. d 3D super-resolved z-slice (100–200 nm) of the highlighted area in a. e 3D super-resolved z-slice (200–300 nm) of the highlighted area in a. f 3D super-resolved z-slice (300–400 nm) of the highlighted area in a. g 3D super-resolved z-slice (400–500 nm) of the highlighted area in a. Scale bars, 5 µm (a), 500 nm (bg)

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