Fig. 2 | Nature Communications

Fig. 2

From: Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Fig. 2

Whole-cell DNA- and Exchange-PAINT enabled by SDC microscopy. a Schematic drawing of the cell and the detection volume for z positions 0 µm (left), 1 µm (middle), and 4 µm (right). b 500-nm slice of the microtubule network in a HeLa cell at the coverslip surface. Left: super-resolved DNA-PAINT image. Right: super-resolved (SR) and diffraction-limited (DL) zoomed-in image of the highlighted area. c 500-nm slice of the same cell ~1 µm away from the coverslip surface. d 500 nm slice of the same cell ~4 µm away from the coverslip surface. eg Three-target Exchange-PAINT of Alpha-Tubulin (green), TOM20 (red), and HSP60 (blue). e Left: DNA-PAINT super-resolution image of a 500-nm slice at the coverslip surface. Right: super-resolved (SR) and diffraction-limited (DL) zoomed-in image of the highlighted area. f 500 nm slice of the same cell ~1 µm away from the coverslip surface. g 500-nm slice of the same cell ~4 µm away from the coverslip surface. Scale bars, 5 µm (bg), 500 nm (insets in b and e)

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