Fig. 1 | Nature Communications

Fig. 1

From: Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Fig. 1

DNA-PAINT with sub-15-nm lateral and sub-50-nm axial resolution using spinning disk confocal (SDC) microscopy. a Different illumination and detection schemes. Total internal reflection fluorescence (TIRF) creates an evanescent field at the surface. Highly inclined and laminated optical sheet (HILO) enables sheet-like illumination for deeper imaging. Spinning disk confocal (SDC) detects emission light only from the focal plane (red). b Schematic drawing of an SDC where 3D super-localization is achieved by optical astigmatism through a cylindrical lens in the detection path. c DNA origami structures imaged with DNA-PAINT on an SDC. Diffraction-limited (DL) and super-resolution (SR) representation. The schematic inset shows the DNA origami structure with docking strands in each corner. d SDC–DNA-PAINT allows sub-20 nm resolution. Schematic depiction of the DNA origami structure (top left). Super-resolution sum image of 1028 structures (bottom right) along with histograms of columns and rows of the DNA origami. The measured distances are in good agreement with the designed distances. e Lateral 2D projection of DNA origami tetrahedrons (designed length of each edge is 100 nm). Diffraction-limited image (DL) and DNA-PAINT super-resolved image (SR). f Isometric 3D sum image of 42 tetrahedrons (inset: design of tetrahedron). This panel represents an isometric 3D sum image as overview image of the 3D tetrahedron. g xy projection of the summed image. h xz projection of the summed image. i Height measurement of the tetrahedrons obtained from the cross-sectional histogram in the x–z projection from h yields 80 nm. Scale bars, 200 nm (c, e), 20 nm, d, 50 nm (g, h). Height scale, 0–200 nm (f–h)

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