Fig. 4 | Nature Communications

Fig. 4

From: The mitochondrial negative regulator MCJ is a therapeutic target for acetaminophen-induced liver injury

Fig. 4

Lack of MCJ counteracts APAP toxicity in hepatocytes by maintaining mitochondrial function and ATP levels. Triplicates were used for experimental condition. a Total ATP levels in WT and MCJ KO primary hepatocytes treated with APAP for 9 h. b Oxygen consumption rate (OCR) and c mitochondrial ATP production using the Mitostress assay at basal conditions and in the presence of APAP in WT and MCJ KO primary hepatocytes. d ATP levels in mitochondria isolated from WT and MCJ KO livers after 24 h of APAP administration. eg WT mice were administered with APAP 360 mg/kg (n = 12), 24 h later mice (n = 4) received siMCJ and 12 h later mice were harvested and livers were used to examine e MCJ silencing by western blotting, f liver necrosis by H&E staining, and g PCNA expression by immunohistochemistry. Scale bar corresponds to 100 μm. Values are represented as mean ± SEM. *P < 0.05, ***P < 0.001 (Student’s t test) (APAP vs. Control, MCJ KO vs. WT, and siMCJ vs. Control)

Back to article page