Fig. 3 | Nature Communications

Fig. 3

From: The mitochondrial negative regulator MCJ is a therapeutic target for acetaminophen-induced liver injury

Fig. 3

MCJ liver-specific silencing prevents against APAP-induced liver injury in vivo. af WT mice were treated with APAP 360 mg/kg and 6 h after control (Control) (n = 6) or a MCJ-specific siRNA (siMCJ) (n = 6) were intravenously injected. a MCJ silencing was evaluated by western blotting, b liver necrosis was assessed by H&E staining, c ROS in vivo measured by DHE staining in liver sections, d inflammation assessed by F4/80 staining in liver, e relative mRNA expression of different inflammatory genes, f serum ALT levels in Control and siMCJ-treated animals. gk WT mice were treated with APAP 360 mg/kg and 24 h after control (Control) (n = 6) or a MCJ-specific siRNA (siMCJ) (n = 6) were intravenously injected. g Liver necrosis was assessed by H&E staining, h ROS in vivo measured by DHE staining in liver sections, i inflammation assessed by F4/80 staining in liver, j serum ALT, and k serine aminotransferase (AST) levels in Control and siMCJ-treated animals. Scale bar corresponds to 100 μm. Values are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test) (siMCJ vs. Control)