Fig. 1 | Nature Communications

Fig. 1

From: Cisplatin is retained in the cochlea indefinitely following chemotherapy

Fig. 1

A clinically relevant mouse model of cisplatin ototoxicity shows progressive, high-frequency hearing loss. a The three-cycle cisplatin regimen administered to CBA/CaJ mice. Each cycle consisted of 4 days of once-daily i.p. injections of cisplatin followed by 10 days of recovery. b Hearing loss following the cisplatin regimen as measured by auditory brainstem response recordings. Cisplatin caused a moderate to severe hearing loss across frequencies. n = 22 mice for cisplatin and saline groups. n = 6 mice for 60 days after cisplatin and saline groups. Statistical significance as determined by two-way ANOVA followed by the Holm–Šidák multiple comparisons test is displayed for cisplatin vs. saline. c Progression of hearing loss after each cycle of cisplatin and subsequent recovery. High-frequency hearing loss becomes evident after two cycles. Robust hearing loss across all frequencies is evident after three cycles. n = 3–5 for each cisplatin treatment time point, n = 23 for saline group. Two-way ANOVA followed by the Holm–Šidák multiple comparisons test was used to determine significance. d Percentages of surviving inner and outer hair cells (as compared to control averages) following the complete cisplatin regimen. Predominantly outer hair cells in the basal portion of the cochlea are lost. n = 3 mice in each group. e Distortion product otoacoustic emission (DPOAE) amplitudes (a measure of outer hair cell function) after each cycle and recovery period. Gray shaded area depicts the testing noise floor (the underlying background noise detected by the system microphone). n = 3–5 mice at each time point. Two-way ANOVA followed by the Holm–Šidák multiple comparisons test was used to determine significance. Data are expressed as mean ± s.e.m. ns not significant, *P < 0.01, **P < 0.01, ****P < 0.0001

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