Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation

Zhu, Liang; Yang, Jun; Bromberger, Thomas; Holly, Ashley; Lu, Fan; Liu, Huan; Sun, Kevin; Klapproth, Sarah; Hirbawi, Jamila; Byzova, Tatiana V.; Plow, Edward F.; Moser, Markus; Qin, Jun

doi:10.1038/s41467-017-01822-8

Download PDF

Article
Open access
Published: 23 November 2017

Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation

Liang Zhu^1,2^na1,
Jun Yang¹^na1,
Thomas Bromberger³^na1,
Ashley Holly¹,
Fan Lu^1,2,
Huan Liu¹,
Kevin Sun¹,
Sarah Klapproth³,
Jamila Hirbawi¹,
Tatiana V. Byzova¹,
Edward F. Plow¹,
Markus Moser³ &
…
Jun Qin^1,2

Nature Communications volume 8, Article number: 1744 (2017) Cite this article

5015 Accesses
72 Citations
9 Altmetric
Metrics details

Subjects

Abstract

Activation of transmembrane receptor integrin by talin is essential for inducing cell adhesion. However, the pathway that recruits talin to the membrane, which critically controls talin’s action, remains elusive. Membrane-anchored mammalian small GTPase Rap1 is known to bind talin-F0 domain but the binding was shown to be weak and thus hardly studied. Here we show structurally that talin-F0 binds to human Rap1b like canonical Rap1 effectors despite little sequence homology, and disruption of the binding strongly impairs integrin activation, cell adhesion, and cell spreading. Furthermore, while being weak in conventional binary binding conditions, the Rap1b/talin interaction becomes strong upon attachment of activated Rap1b to vesicular membranes that mimic the agonist-induced microenvironment. These data identify a crucial Rap1-mediated membrane-targeting mechanism for talin to activate integrin. They further broadly caution the analyses of weak protein–protein interactions that may be pivotal for function but neglected in the absence of specific cellular microenvironments.

Mechanism of integrin activation by talin and its cooperation with kindlin

Article Open access 29 April 2022

Talin and kindlin use integrin tail allostery and direct binding to activate integrins

Article Open access 12 December 2023

Molecular basis for integrin adhesion receptor binding to p21-activated kinase 4 (PAK4)

Article Open access 17 November 2022

Introduction

The adhesion of cells to the extracellular matrix (ECM) is essential for regulating a variety of physiological or pathological responses such as platelet aggregation, blood clotting, stroke, wound-healing, and cancer metastasis. Such adhesion critically depends upon integrins, a class of cell surface receptors that are (α/β) heterodimers with each subunit containing a small cytoplasmic tail (CT), a transmembrane segment, and a large extracellular domain. In unstimulated cells, integrin adopts an inactive conformation with low affinity for ECM ligands but, upon agonist stimulation, integrin CT is bound by intracellular proteins, in particular talin and/or kindlin, which trigger global conformational change of the extracellular domain to acquire high affinity for ECM ligand and initiate firm adhesion. This process, widely regarded as inside-out signaling or integrin activation, has been the central topic of cell adhesion research for nearly three decades^1,2,3,4,5,6. Extensive efforts have been focused on investigating the mechanism of integrin activation by talin—a major cytoskeletal protein comprising an N-terminal head domain (talin-H) and a C-terminal rod domain (talin-R). Talin-H contains four subdomains F0, F1, F2, and F3 in which F1–F3 constitutes a so-called FERM (4.1/ezrin/radixin/moesin)-like domain. Talin-R is made up of 13 consecutive helical bundles followed by a C-terminal dimerization domain (Fig. 1). Intact talin adopts an inactive conformation where the major integrin-binding site located in talin-H F3 domain is masked by talin-R (autoinhibition)^7,8,9,10. Talin may be unmasked via multiple mechanisms to bind integrin β CT^{7, 8, 11,12,13} and trigger the receptor activation^14,15,16,17.

While a great deal has been learned about talin, a fundamental issue still remains unresolved: how is cytoplasmic talin recruited to plasma membrane—a crucial step for talin to bind and activate integrin? A previous dogma, largely derived from studying prototypic integrin α_IIbβ₃ from platelets, is that agonist stimulation activates small GTPase Rap1 on the membrane to recruit an effector called RIAM that in turn recruits talin^{5, 18}. However, while both Rap1 and talin are highly abundant¹⁹, RIAM is present at very low levels in platelets with no other homologs detected²⁰. More importantly, mice lacking RIAM are viable and the platelet functions including the α_IIbβ₃ activation are totally normal^20,21,22. By contrast, Rap1 and talin are both essential for integrin activation^{4, 5, 18}. For example, ablation of the Rap1 isoform Rap1b severely impairs α_IIbβ₃ activation and platelet aggregation²³—defects that were also observed in talin knockout mice^{24, 25}. These observations strongly suggest a RIAM-independent engagement between Rap1 and talin to regulate integrin activation. Interestingly, previous studies reported a direct interaction between Rap1 and talin in mammals²⁶ and in Dictyostelium²⁷, but the affinity of the interaction was found to be low especially for that in mammals (K _d ~ 0.14 mM)²⁶. Since weak protein–protein interactions (PPIs) are typically assumed to be nonspecific and RIAM was widely regarded as a key bridge between Rap1 and talin, the Rap1/talin interaction was hardly studied at the mechanistic level. Through comprehensive structural, biochemical, and cell biological analyses, we discovered that talin is actually a distinct effector of Rap1 that recruits talin to membrane and triggers integrin activation. We further found that, while being weak in conventional binary binding assays, Rap1/talin interaction became strong when activated Rap1 was anchored to membrane that mimics the cellular condition. Our results thus uncover a mechanism of membrane-targeting of talin by Rap1 and also highlight the importance of weak PPIs that may be neglected in the absence of specific cellular microenvironments.

Results

Rap1 recognizes talin-F0 domain in a GTP-dependent manner

Rap1 is known to contain two highly homologous isoforms called Rap1a and Rap1b^28,29,30,31. In this study, we choose to focus on examining the interaction between the platelet-rich Rap1b and talin-F0. Like other small GTPases, Rap1b exists in inactive (GDP-bound) and active (GTP-bound) states. We first used a highly sensitive NMR technique called heteronuclear single quantum coherence (HSQC)³² to examine the Rap1b/talin-F0 interaction. Figure 2a and Supplementary Fig. 1a show that Rap1b loaded with GTP analog GMP-PNP induced residue-specific chemical shift changes of ¹⁵N-talin-F0. Inactive Rap1b loaded with GDP also binds to talin-F0 but at weaker affinity as evidenced by smaller chemical shift changes than those with GMP-PNP (Fig. 2b). Thus, talin-F0 binds favorably to the active form of Rap1b but, as shown in Fig. 2c, the binding affinity is quite low (K _d ~ 162 μM) as previously concluded²⁶. On the other hand, talin-F0 does not bind H-Ras (Supplementary Fig. 1b) that is near 90% homologous in terms of binding interface (overall similarity is ~76%) to Rap1b (see also Supplementary Fig. 1c), suggesting that despite being weak, the talin-F0/Rap1b interaction is specific. To further investigate the specificity of Rap1b/talin-F0 interaction, we examined the Rap1b binding to F0 domain of another integrin activator kindlin2 that is homologous to talin-H (Supplementary Fig. 2a)³³, but no interaction was detected (Fig. 2d). Since talin-H contains FERM domain (F1–F3) prior to F0 and F1–F2 of KRIT1 FERM domain was shown to bind Rap1b³⁴, we also wondered whether F1–F2 of talin FERM would be involved in binding to Rap1b. However, Supplementary Fig. 2b shows that Rap1b does not interact with talin FERM F1–F2. Overall, these data suggest that Rap1b engages with talin-F0 in a highly specific manner. Interestingly, NMR (Fig. 3a, Supplementary Figs. 1a and 2c) and Glutathione S-transferase (GST) pull-down (Fig. 3b) experiments revealed that active Rap1b also binds talin-H and full-length talin in the same manner as to talin-F0. Given that full-length talin is autoinhibited with F0 being unmasked⁷, these data suggest that active Rap1b specifically recognizes the F0 domain of resting talin.

Rap1b/talin interface exhibits distinct features

To understand how Rap1b recognizes talin, we decided to pursue the total structure of active human Rap1b (~20 kDa) in complex with talin-F0 (~10 kDa). Solution NMR is particularly suitable for structure determination of weak protein complexes³². By performing a series of three-dimensional (3D) heteronuclear experiments using a combination of ¹H/¹⁵N/¹³C-labeled and ²H/¹⁵N-labeled samples, we were able to obtain nearly complete resonance assignment, 3422 intramolecular NOEs, and 78 intermolecular NOEs (Supplementary Fig. 3), which allowed determination of the complex structure (Fig. 4a and Supplementary Table 1). Figure 4b displays the cartoon diagram of the overall complex in which individual subunits, Rap1b and talin-F0, adopt a Ras family GTPase fold and an ubiquitin-like fold, respectively, as expected. The ubiquitin-like fold of talin-F0 has similarity to Ras-association (RA) domain present in known Rap1 effectors such as KRIT1, RIAM, and c-Raf1 (Fig. 4c). However, despite this similar fold, talin-F0 has little sequence homology with these Rap1b RAs (Fig. 4d) with only three positively charged residues being relatively conserved (highlighted in red in Fig. 4d). The detailed Rap1b/talin-F0 interface is summarized in Fig. 4e and Supplementary Fig. 4a and compared with other Rap1b effectors (Supplementary Table 2). Majority of the contacts in the Rap1b/talin-F0 complex are different from other Rap1b/effector complexes (Supplementary Table 2) apparently due to the scant sequence homology of talin-F0 with other Rap1b effectors (Fig. 4d). A particularly distinct feature in the Rap1b/talin-F0 complex is the existence of a hydrophobic core between I36/P37 of talin-F0 and V21/I27/V29 of Rap1b (Fig. 4e and Supplementary Fig. 4b). Such core was not observed in other Rap1b/effector complexes. To understand how Rap1b but not the highly homologous H-Ras binds talin-F0 (Supplementary Fig. 1b), we mutated I27 and K31 of Rap1b, which closely contact with talin-F0, into corresponding H-Ras residues, Histidine and Glutamate, respectively. Both mutations showed reduced binding to talin-F0 (Supplementary Fig. 4c). By contrast, both Rap1b and H-Ras bind equally well to RIAM as shown before³⁵ as also confirmed in Supplementary Fig. 4d and Supplementary Fig. 16. These data provide insight into the high specificity of the talin binding to Rap1b despite the weaker affinity than that between talin and RIAM (Supplementary Fig. 4e).

Further analysis revealed that, while the Rap1b/talin-F0 interface exhibits distinct features (Fig. 4e, Supplementary Fig. 4a, and Supplementary Table 2), the overall topology of the complex shares similarity with other Rap1b/effector complexes (Fig. 4b, c). A key determinant for this similarity appears to be the antiparallel β-sheet between Rap1b β2 strand and talin-F0 β2 strand (Fig. 4b and Supplementary Fig. 4a), as also present in other Rap1b/effector complexes (Fig. 4c). In addition, charge–charge interactions in the talin-F0/Rap1b complex, which involve talin-F0 K7 with Rap1b E37 and talin-F0 K15/R35 with Rap1b D33, are relatively conserved in other Rap1b/effector complexes (Fig. 4d and Supplementary Table 2). By contrast, kindlin2-F0, which is not a Rap1b effector (Fig. 2d) but is highly homologous to talin-F0 (Supplementary Fig. 2a), lacks two positively charged residues in the corresponding positions (Fig. 4d).

In summary, the Rap1b/talin-F0 interface is quite unique yet resembles other Rap1b/effector complexes in overall topology. These structural and binding data suggest that talin is a direct effector of Rap1. Interestingly, the interface residues are highly conserved in both talin/Rap1 isoforms and in different species (Supplementary Fig. 5a, b), suggesting that Rap1/talin recognition is evolutionally conserved.

Rap1b binding to talin is crucial for integrin activation

To further evaluate the importance of the Rap1b/talin interaction, we performed the structure-based analysis and mutated two critical interface residues K15 and R35 of talin-F0 (Fig. 4e and Supplementary Fig. 4a) into alanines. Figure 5a and Supplementary Fig. 6 show that K15A/R35A double mutations (DM) drastically reduced the Rap1b interaction to talin-F0 as well as talin-H. Since talin-H is the unit responsible for binding and activating integrin^{5, 36}, we next tested the impact of the Rap1b-binding defective mutations on its ability to activate α_IIbβ₃—the prototypic integrin widely used to study integrin activation. To this end, we transfected wild-type (WT) talin-H or talin-H DM into Chinese hamster ovary (CHO) A5 cells stably expressing α_IIbβ₃ (Supplementary Fig. 7a). Figure 5b shows that, as compared to the WT talin-H, the talin-H mutant-induced α_IIbβ₃ activation was substantially reduced. These results provide strong evidence that the interaction of talin-H with Rap1b is crucial for the talin-H-mediated integrin activation. They also explain a previously reported phenomenon where deletion of F0 domain in talin-H significantly impaired the integrin activation³⁷. To further demonstrate that the impaired interaction between talin DM and Rap1 has an impact on integrin-mediated functions, we retrovirally transduced talin-null fibroblasts (talin^1/2dko), which show a strong defect in cell adhesion and spreading³⁸, with C terminally ypet-tagged talin WT as well as talin DM. The transduced cells were FACS-sorted for equal talin and talin DM expression as confirmed by flow cytometry and western blotting (Supplementary Figs. 7b and 17). Re-expression of both WT talin and talin DM induced cell spreading of talin^1/2dko cells with WT and mutant talin localizing to paxillin-positive focal adhesions (FAs; Supplementary Figs. 7c, d). We then performed adhesion assays on various integrin ligands and observed that talin DM only partially rescued cell adhesion of talin^1/2dko cells to fibronectin (FN), laminin-111 (LN), and vitronectin (VN) compared to WT talin, whereas integrin-independent adhesion to poly-L-lysine was unchanged (Fig. 5c). As expected, talin^1/2dko cells expressing ypet as a control hardly adhered to any integrin ligand (Fig. 5c). Importantly, expression of mutant talin did not alter the expression levels of surface integrins nor those of RIAM and Rap1 (Supplementary Figs. 7b, e). Next, we plated the cells on FN and measured cell spreading for 4 h. While ypet-transduced talin^1/2dko cells remained roundish and did not spread, talin DM cells displayed a significant spreading defect compared to talin WT-transduced cells (Fig. 5d).

We hypothesize that the reduced PAC1 binding, cell adhesion, and spreading of talin DM-expressing cells are due to impaired integrin activation caused by deficient talin recruitment to the integrin site at the plasma membrane. To test this hypothesis experimentally, we seeded WT talin and talin DM-expressing cells on FN-coated micropatterns for 4 h and measured the number and size of paxillin-positive FAs. This analysis reveals that indeed the FA area and number per cell are significantly reduced in cells expressing talin DM compared to those expressing WT talin (Figs. 5e–g). The mean FA size (Fig. 5h) was also reduced by talin DM, although with a p value of 0.09. To further test whether impaired talin/Rap1 interaction affects the recruitment of talin DM to FAs, we measured the intensity of ypet fluorescence within FAs and correlated it to the total cellular ypet fluorescence intensity. We observed a significantly reduced relative intensity of ypet signal within FAs in talin DM-transduced cells, which suggests that the talin/Rap1b interaction is important for talin recruitment to FAs (Fig. 5i). Collectively, our data provide strong evidence for the physiological importance of direct Rap1/talin interaction in integrin regulation.

Membrane-anchored Rap1b shows enhanced binding to talin

While the forgoing data demonstrated the unique specificity of Rap1b/talin interaction and its importance in regulating integrin-mediated functions, a key issue still remains: how can such interaction with ~0.16 mM affinity be effective since protein concentration in cells is typically much lower than 0.16 mM? We attempted to address this issue from two angles. First, both Rap1 and talin are highly abundant in cells. For example, recent proteomic studies showed that Rap1b and talin belong to the top most abundant proteins in platelets with copy numbers of ~200,000, which are nearly 1/4 of the most abundant platelet protein actin¹⁹. The high levels of Rap1 and talin in platelets are consistent with our protein expression analysis (Fig. 6a). Given that the average concentration of actin is above 200 μM in platelets³⁹, the estimated concentration of Rap1b or talin in platelets should be at least 50 μM. By contrast, the RIAM level is very low in both human and murine platelets (Fig. 6a), which was also confirmed by quantitative mass spectrometry with a copy number of only 162¹⁹. Thus, the high concentrations of Rap1 and talin increase the chance for their direct interaction to occur. Second, given that Ras family GTPases including Rap1 are all known to be attached to membrane via Cys prenylation of their C-terminal CAAX motif²⁹ and talin-H also binds to membrane via a large positively charged surface on F1, F2, and F3 domains^{8, 40}, we reasoned that Rap1 and talin-H might be more tightly associated if Rap1 were prenylated with membrane to mimic the true cellular microenvironment (Fig. 6b). Our Rap1b/talin-F0 structure superimposed with the structure of talin-H supports this possibility in that the CAAX containing C terminus of Rap1b and the positively charged surface of talin-H align well to all face toward the membrane surface (Fig. 6b). To address this possibility experimentally, we prepared membrane vesicles with anchored Rap1b using the well-established thiol-maleimide crosslinking method⁴¹ (Supplementary Fig. 8a). As shown in Fig. 6c, membrane-anchored Rap1b indeed bound much more robustly to talin-H than Rap1b unanchored to membrane. Isothermal titration calorimetry (ITC) experiments revealed a K _d of ~1.52 μM for membrane-anchored active Rap1b to bind to talin-H (Supplementary Fig. 8b), which indicates a more than two orders of magnitude stronger binding than that measured without membrane (Fig. 2c and Supplementary Fig. 4e). Consistently, the membrane-binding capacity of talin-H was also substantially enhanced by Rap1 anchored to the membrane, but such enhancement was diminished for talin-H DM that is defective for binding to Rap1 (Fig. 6d). Importantly, the binding of membrane-anchored Rap1b to talin-H is GTP-dependent as shown by two independent methods: GST pull-down assay (Supplementary Figs. 8c and 18) and co-sedimentation assay (Supplementary Figs. 9a and 19). Such GTP dependence indicates that the effective membrane targeting of talin requires active Rap1b. These findings provide key evidence for talin being a bona fide Rap1 effector and suggest how agonist-activated and membrane-anchored Rap1 can effectively recruit talin to the membrane surface. Although full-length talin binds to membrane more weakly than talin-H possibly due to some degree of autoinhibition for the former, membrane with anchored Rap1b clearly binds more potently to full-length talin contrasting to the scenario where Rap1b was not attached to the membrane (Fig. 6e). Similar to membrane-mediated Rap1b/talin-H binding (Supplementary Fig. 9a), membrane-anchored Rap1b also interacts with full-length talin in a GTP-dependent manner (Supplementary Fig. 9b). Importantly, since full-length talin adopts an autoinhibited conformation^{7, 10}, which may be unmasked through a pull–push mechanism by binding to the membrane⁸, our data further suggest that Rap1 not only promotes the membrane targeting but also leads to activation of talin via this mechanism (Fig. 6b). In order to test this hypothesis, we performed NMR-based competition experiments by examining the interaction of ¹⁵N/²H-labeled inhibitory talin rod domain 9 (talin-R9) with talin-H in the absence and presence of membrane-anchored Rap1b. Supplementary Fig. 9c shows that, while talin-H induces residue-specific chemical shift changes of talin-R9 as the signature of the autoinhibition⁸, such chemical shift changes were suppressed by membrane-anchored Rap1b, thus demonstrating the talin-unmasking process.

Discussion

Rap1 was discovered nearly three decades ago⁴², and its two isoforms Rap1a and Rap1b (with ~95% identity, see also Supplementary Fig. 5b) are regarded as the key membrane-associated small GTPases to target proteins to the plasma membrane to regulate diverse cellular responses^28,29,30,31. A large body of data have indicated that Rap1 is critically involved in activation of integrins^{5, 18, 43}, but the immediate downstream effector of Rap1 has been highly elusive. Our studies have now obtained an important clue on this longstanding mystery. We found that the activated Rap1b interacts with the F0 domain of talin—the key mediator of integrin activation, and we determined the NMR structure of the Rap1b/talin-F0 complex. Despite little sequence homology between talin-F0 and other Rap1 effectors, the overall topology of the complex was found to resemble those known Rap1/effector complexes. Moreover, disruption of the Rap1b/talin interface substantially impaired the integrin α_IIbβ₃ activation, cell adhesion, and cell spreading. Interestingly, we found that, while being weak measured in conventional binary conditions²⁶, the Rap1b/talin interaction became quite strong when Rap1b was anchored to membrane—a condition mimicking the cellular microenvironment of Ras family GTPases⁴¹ and also favoring talin unmasking as indicated previously⁸. Overall, our data suggest a crucial pathway by which agonist-activated Rap1 directly promotes the membrane recruitment of talin, which further leads to the talin unmasking and integrin activation (Fig. 6b). Our data also provide an explanation of why Rap1 and talin are essential for integrin activation, whereas RIAM is dispensable for activation of most integrins expressed on many cell types as indicated by the recent series of genetic studies^20,21,22. On the other hand, RIAM, which is highly abundant in leukocytes, was shown to play an important role in regulating leukocyte integrin function^{21, 22} by possibly forming the so-called “MIT complex” with talin and integrin⁴⁴, indicating that the direct Rap1/talin pathway plays a minor role in regulating the leukocyte β₂ integrins. The fact that talin DM still partially supports cell adhesion and spreading of talin^1/2dko cells (Fig. 5c, d) further indicates the existence of other compensatory pathways including “Rap1-RIAM-talin” and “PIPKIγ/PIP2-talin”^{7, 8, 11}. More detailed investigations are needed to understand how these different pathways are turned on or cooperated in a spatiotemporal manner. Nevertheless, our data, combined with the genetic evidence of Rap1 and talin in integrin activation, suggest that direct Rap1–talin interaction critically regulates integrin activity.

In addition to uncovering the physiological role of the Rap1b/talin interaction, our findings also bear broad implications in the analyses of PPIs. Tens of thousands of PPIs in cells form a web of intricate communication networks for regulating diverse biological processes⁴⁵. Interestingly, recent proteomic analyses have suggested that weak PPIs dominate in these cellular PPI networks⁴⁶. However, because cellular protein concentrations, which are typically estimated on whole-cell volumes, are low (e.g., nM), weak binary PPIs, as detected by conventional in vitro methods, were commonly treated to be nonspecific and therefore disregarded for further investigations. Nevertheless, these weak PPIs may be enhanced and they exert crucial functions at specific microenvironments, which is strongly demonstrated in our study. Specifically, while isolated Rap1b and talin-F0 binds at sub-mM affinity, the binding became very strong in the presence of membrane that mimics the cellular microenvironment. As illustrated in Fig. 6b, the membrane-anchoring ability of both Rap1b and talin may increase the local concentration of both proteins, thereby markedly strengthening their association. Other types of cellular strategies may also exist to enhance the association of otherwise weak binary interactions, which remain to be further investigated. With the completion of human genome and rapidly growing data of weak PPIs⁴⁶, our study emphasizes cautious treatment of a wide variety of weak PPIs by including specific microenvironments. In this regard, NMR is particularly valuable for deciphering the detailed structural basis of weak PPIs³² in vitro, laying down the foundation for further analysis of the interaction in certain networks both in vitro and in vivo, as exemplified in our study. Such approach would complement with other techniques such as crystallography and CryoEM that are advantageous for elucidating large high-affinity complexes, together, facilitating a thorough and unbiased view of how proteins function in cells.

Methods

Plasmid constructs and mutagenesis

The following constructs for bacterial expression were used in this study: mouse talin-F0 (1–86) subcloned into a pHis-1 vector, mouse full-length talin subcloned into a pET28t vector, human kindlin2-F0 (1–105) subcloned into a pGST-1 vector, mouse talin-H (1–429) subcloned into a pET28t, and a pGST-1 vector, mouse RIAM-RAPH (149–438) subcloned into a pGST-1 vector, human full-length Rap1b subcloned into a pGST-1 vector, and C-terminal truncated Rap1b (1–167) subcloned into a pET28t vector. Human wild-type Rap1b and H-Ras (G12V) subcloned into pET28a vectors (6xHis tag) were kind gifts from Dr. Matthias Buck at Case Western Reserve University. Construct mutagenesis was conducted by using QuickChange lightning site-directed mutagenesis Kit (Agilent Technologies). Mutated constructs generated in this study include talin-F0_DM (K15A, R35A), talin-H_DM (K15A, R35A), Rap1b (G12V), Rap1b (1–167, G12V), Rap1b (G12V, I27H), Rap1b (G12V, K31E), Rap1b_TM1 (C51S, C118S, C141S), and Rap1b_TM2 (G12V, C51S, C118S, C141S). Note that pET28t is a modified pET28a vector where the thrombin cleavage site is substituted with a TEV protease cleavage site. Primers used in this study are listed in Supplementary Table 3.

Protein expression and purification

Recombinant proteins with fusion tag (6xHis tag or GST tag) were expressed in Escherichia coli BL21 (DE3) strain (New England Biolabs). Typically, bacteria was initially grown in 50 ml Lysogeny broth (LB) medium and then amplified in 1 ~ 2 l LB at 37 °C. The culture was induced by 0.4 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 20 °C or room temperature (RT) for overnight when it reached an A₆₀₀ of 0.7. The pellet was then collected and suspended in buffer and frozen at −80 °C. For protein purification, the pellet was lysed by incubation with lysozyme and sonication. After high-speed centrifugation, the supernatant was subjected to affinity column purification by using either nickel or GST gravity column. Ion exchange chromatography was an optional purification step depending on protein purity (in this study, full-length Rap1b was purified by anion exchange method using Hi-trap Q column). Gel filtration was always performed in the final step by using either Superdex-75 (16/60) or Superdex-200 (16/60; GE Healthcare). Note that Superose-6 (10/300) was used for full-length talin purification. Purified protein was checked by SDS-PAGE. Proteins with 90% purity or above were used in this study. According to experimental requirement, fusion tag (either His-tag or GST tag) was removed by TEV protease during protein purification if necessary. Isotope-labeled (¹⁵N and/or ¹³C) proteins were achieved by employing minimal medium with ¹⁵NH₄Cl and/or ¹³C-glucose as the sole nitrogen and carbon sources. ²H-labeled protein was achieved by using the minimal medium prepared in 99.8% D₂O with ²H-glucose as the carbon source. Protein concentration was measured by absorbance at 280 nm or Pierce 660 nm protein assay reagent (Thermo Fisher Scientific).

GTPase nucleotide exchange

Non-hydrolyzable analogs of GDP and GTP, named Guanosine 5′-[β-thio] diphosphate trilithium salt (GDP-β-S) and Guanosine 5′-[β, γ-imido] triphosphate trisodium salt (GMP-PNP), respectively, were purchased from Sigma-Aldrich. In this study, wild-type Rap1b loaded with GDP-β-S was used as an inactive form, and Rap1b (G12V) or H-Ras (G12V) loaded with GMP-PNP was used as an active form. Purified wild-type Rap1b (50 μM), Rap1b (G12V) or H-Ras (G12V) was first incubated with 2 mM EDTA and 2 mM GDP-β-S or GMP-PNP at RT for 35 min, MgCl₂ was then added in to reach a final concentration of 7 mM and incubated for another 30 min. After that, they were kept on ice and concentrated into a high concentration for experimental use.

NMR 2D-HSQC and HSQC titration

2D-HSQC experiments were performed on Bruker 600 MHz NMR spectrometer, and samples containing 50 μM ¹⁵N-labeled protein were studied. For ¹⁵N-labeled talin-F0 or kindlin2-F0, experiments were performed at 25 °C in buffer containing 20 mM NaH₂PO₄/Na₂HPO₄ (pH 6.6), 50 mM NaCl, 5 mM MgCl₂, 2 mM dithiothreitol (DTT), and 5% D₂O. For ¹⁵N-labeled Rap1b (1–167), experiments were performed at 25 °C in buffer containing 25 mM NaH₂PO₄/Na₂HPO₄ (pH 6.8), 150 mM NaCl, 5 mM MgCl₂, 2 mM DTT, and 5% D₂O. Chemical shift change (Δδ _{obs [HN,N]}) was calculated with the equation Δδ _{obs [HN,N]} = [(Δδ _HN W _HN)² + (Δδ _N W _N)²)]^1/2, where W _HN and W _N are weighting factors based on the gyromagnetic ratios of ¹H and ¹⁵N (W _HN = 1 and W _N = 0.154) and Δδ (p.p.m.) = δ _bound – δ _free. For HSQC titration, samples containing 45 μM ¹⁵N-labeled talin-F0 with increasing amount of GMP-PNP-loaded Rap1b (G12V) were used to collect 2D-HSQC spectra. Dissociation constant (K _d) was achieved by fitting the chemical shift changes into the equation as shown below⁴⁷. Four independent well-resolved residues were selected for fitting and an average K _d was obtained.

∆δ_obs = ∆δ_max{(K _d + [P]_t + [L]_t) − [(K _d + [P]_t + [L]_t)² − 4 × [P]_t × [L]_t]^1/2} / 2[P]_t (∆δ_max, maximum chemical shift change; [P]_t, labeled protein concentration; [L]_t, ligand or titrant concentration).

GST pull-down

A volume of ~ 25 μg purified GST or GST-fused protein was immobilized on 15 μl glutathione-Sepharose 4B resin (beads) via incubation on a rotator for 1.5 h at 4 °C in the binding buffer containing 25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 0.01% Nondiet P-40, and supplemented with Complete EDTA-free Protease Inhibitor (Roche, Indianapolis, IN). Desired amount of prey protein was then added in and incubated with beads for another 2 h at 4 °C. After that, the beads were washed by 600 μl binding buffer twice and subjected to be denatured by adding 35 μl 2 × SDS loading buffer and boiling for 5 min. After centrifuging down, supernatants were resolved by SDS-PAGE or western blotting. All the pull-down experiments were performed in triplicate independently.

Western blotting and antibodies

After SDS-PAGE, samples were transferred onto a 0.45 μm polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA). The membrane was blocked with 5% non-fat milk in TBST buffer overnight at 4 °C. After that, the membrane was incubated with primary antibody at RT for 3 h and then incubated with horeseradish peroxidase (HRP)-conjugated secondary antibody at RT for 1 h. The blots were detected by Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). The following primary antibodies were used in this study: 6xHis epitope tag antibody (Catalog# MA1-21315, Thermo Fisher Scientific), Rap1a/Rap1b (26B4) Rabbit mAb (Catalog# 2399, Cell Signaling Technology), Ras (27H5) Rabbit mAb (Catalog# 3339, Cell Signaling Technology), GAPDH (D16H11) Rabbit mAb (Catalog# 5174, Cell Signaling Technology), Anti-Talin-1 antibody (Catalog# ab57758, Abcam Inc.), and Anti-RIAM antibody (EPR2806; Catalog# ab92537, Abcam Inc.). The following secondary antibodies were used in this study: anti-mouse IgG, HRP-linked antibody (Catalog# 7076, Cell Signaling Technology), and anti-rabbit IgG, HRP-linked antibody (Catalog# 7074, Cell Signaling Technology). Primary antibodies were used at 1:1000 dilution, and secondary antibodies were used at 1:3000 dilution.

Assignment of Rap1b and talin-F0 and their bound forms

C-terminal truncated Rap1b (1–167) with G12V mutation was used in all the following structure determination studies. For assignment of Rap1b, ¹⁵N/¹³C-labeled Rap1b was purified and the N-terminal 6xHis tag was removed during purification. Standard triple-resonance experiments were conducted with 0.6 mM GMP-PNP-loaded Rap1b. Rap1b sequential assignment was performed using PASA software⁴⁸. For assignment of talin-F0, the complete chemical shift–assignment table of talin-F0 from Biological Magnetic Resonance Data Bank (accession code 15458) was downloaded and used in this study²⁶. Amide proton to nitrogen to α-carbon correlation (HNCA) experiment was performed on 1.0 mM ¹⁵N/¹³C-labeled talin-F0 to confirm the assigned resonances. All these experiments were performed on Bruker 600 MHz NMR spectrometer, and at 25 °C in the buffer that contained 20 mM NaH₂PO₄/Na₂HPO₄ (pH 6.6), 50 mM NaCl, 5 mM MgCl₂, 2 mM d-DTT, and 5% D₂O. For assignments of the bound Rap1b and talin-F0 forms, the chemical shifts of majority of residues were readily transferred from the free forms with slight adjustments as they are similar to those of the free forms. A few residues (e.g., I27, R41) exhibited duplex peaks in the free form of Rap1 likely due to local conformational flexibility, and most of these peaks were merged into single peaks in the complex. All assignments were also verified by through-bond NOESY experiments of the complex.

3D-NOESY experiments

The following samples were prepared: (1) 0.6 mM ¹⁵N/¹³C-labeled Rap1b in the presence of 0.9 mM unlabeled talin-F0, (2) 0.5 mM ¹⁵N/¹³C-labeled talin-F0 in the presence of 0.7 mM unlabeled Rap1b, (3) 0.5 mM ¹⁵N/100% ²H-labeled talin-F0 in the presence of 0.7 mM unlabeled Rap1b, and (4) 0.5 mM ¹⁵N/¹³C-labeled talin-F0 in the presence of 0.7 mM unlabeled Rap1b prepared in 99.8% D₂O. The following NOESY experiments were performed using the above samples: (i) 3D ¹⁵N/¹³C-edited NOESY experiment (120 ms mixing time) with sample 1, (ii) 3D ¹⁵N/¹³C-edited NOESY experiment (120 ms mixing time) with sample 2, (iii) 3D ¹⁵N-edited NOESY experiment (300 ms mixing time) with sample 3, and (iv) 3D ¹⁵N/¹³C-filtered NOESY experiment (120 ms mixing time) with sample 4. All these experiments were performed on Bruker 850 or 900 MHz spectrometers at the same condition as mentioned above.

Structure determination

All NMR data were processed and analyzed using nmrPipe⁴⁹, PIPP⁵⁰, and Sparky⁵¹. Distance restraints (NOE constraints) were obtained from 3D-NOESY experiments mentioned above, and Xplor-NIH⁵² was used to calculate the complex structure. In brief, the individual structures of Rap1b and talin-F0 in complex form were calculated first based on the NOEs collected from sample 1 (2308 NOEs) and sample 2 (1114 NOEs), respectively. 302 dihedral-angle restraints for Rap1b derived from TALOS⁵³ were applied during Rap1b structure calculation. The initial templates for Rap1b and talin-F0 were derived from PDB database with PDB codes of 4DXA and 3IVF, respectively. A total of 78 unambiguous intermolecular NOEs collected from sample 3 and sample 4 were used to calculate complex structure (also see Supplementary Table 1 for structural statistics). After refinements, a total of 50 final structures were calculated and the 20 lowest-energy structures were selected for analysis. The quality of structure was evaluated with PROCHECK⁵⁴. Structures are visualized by PyMOL 1.3 (Schrödinger LLC.).

Membrane-anchored Rap1b preparation

To prepare large unilamellar vesicles (LUVs), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P₂), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidomethyl)cyclohexane-carboxamide] (16:0 PE MCC) were purchased from Avanti Polar Lipids. LUVs, which consist of 96% POPC, 1% PIP₂, and 3% PE MCC, were prepared by extrusion. In brief, lipids were first dissolved together in chloroform, and then the chloroform was removed under a stream of nitrogen followed by overnight vacuum pumping. The lipid film was suspended in buffer containing 25 mM NaH₂PO₄/Na₂HPO₄ (pH 6.8), 100 mM NaCl, and 5 mM MgCl₂, and subjected to homogenization with a few freeze–thaw cycles. LUVs were finally formed by extruding the lipid suspension ~20 times through two stacked 0.1 mm polycarbonate filters. Rap1b was tethered to LUVs via Michael addition between the thiol group of Cys 180 and the maleimide group of PE MCC^{41, 55}. To mimic the physiological condition that only the C-terminal Cys 180 of Rap1b is attached to membrane and meanwhile to avoid nonspecific chemical interactions, all the cysteines of Rap1b except Cys 180 were mutated to serines. Rap1b_TM1 (C51S, C118S, and C141S) and Rap1b_TM2 (G12V, C51S, C118S, and C141S) were generated, purified, and loaded with GDP-β-S and GMP-PNP, respectively, as described above. Rap1b_TM2 was first confirmed to interact with ¹⁵N-talin-F0 by HSQC (Supplementary Fig. 10a). To make Rap1b-anchored LUVs, Rap1b_TM1 or Rap1b_TM2 was incubated with freshly prepared LUVs in 1:6 molar ratio overnight at RT in the same buffer. The reaction was terminated by adding β-mercaptoethanol to a final concentration of 5 mM. The achievement of membrane-anchored Rap1b was confirmed by Native-PAGE combined with SDS-PAGE (Supplementary Figs. 10b and 20). Unanchored Rap1b was dialyzed out in the same buffer using a dialyzer with 100 kDa cutoff membrane (Harvard Apparatus) before use.

Vesicle co-sedimentation

Multilamellar vesicles (MLVs) rather than LUVs are used in this study since MLVs are larger in size and could be pelleted down easily. MLVs, which consist of 87% POPC, 10% PIP2, and 3% PE MCC were prepared similarly to LUVs as described above but without extrusion. MLVs with anchored Rap1b (either GDP-β-S or GMP-PNP) were also prepared similarly as described above. Talin-H (10 μM) or full-length talin was incubated with 10 μM “MLV-Rap1b (Rap1b-anchored MLVs)”, “MLV only”, and “MLV + Rap1b (MLVs with unanchored Rap1b)” separately in a “7 × 21 mm” Polycarbonate tube (Beckman Coulter Inc.) with a total volume of 30 μl at RT for 15 min. Samples were then centrifuged down at 22,000 r.p.m. for 35 min. Membrane pellet was dissolved in 2 × SDS loading buffer and subjected to SDS-PAGE analysis. Gels were stained with Coomassie blue, and then scanned and quantified with an Odyssey CLx Imaging System (LI-COR Inc.). The intensities of protein bands were quantified by the fluorescent signal detected from the 700 nm channel according to the manufacturer’s protocol. Experiments were performed in quadruplets. Statistical significance was tested by two-tailed unpaired t-test.

Isothermal titration calorimetry

ITC experiments were performed using a Microcal iTC 200 system (GE Healthcare Life Sciences) at 25 °C. Before experiments, all samples were dialyzed into the same buffer that contained 25 mM NaH₂PO₄/Na₂HPO₄ (pH 6.8), 100 mM NaCl, and 5 mM MgCl₂ overnight at 4 °C. Talin-H (200 μM) in the syringe (~ 40 μl) was injected 20 times in 2.0 μl aliquots into the sample-cell (~220 μl) containing 15 μM LUV-Rap1b (GMP-PNP). As controls (Supplementary Fig. 10c), talin-H was titrated into LUV only, Rap1b only, or buffer only, and talin-H_DM was titrated into LUV-Rap1b (GMP-PNP) using the same condition. ITC Data were analyzed by fitting to a single-site binding model with Origin Software.

Microscale thermophoresis analysis

The affinity of the interaction between GMP-PNP-loaded GFP-Rap1b (G12V) and RIAM-RAPH, talin-F0 or talin-H was measured by using a Monolith NT.115 instrument (NanoTemper Technologies). Rap1b (G12V) was subcloned into a pRSET vector (Thermo Fisher Scientific), which expresses an N-terminal 6xHis tag and a C-terminal GFP fusion tag. RIAM-RAPH, talin-F0, or talin-H was prepared at different concentrations through 1:1 serial dilution and then mixed with 100 nM GFP-Rap1b (G12V) in buffer containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 5 mM MgCl₂, 2 mM DTT, and 0.05% Tween 20. The samples were incubated at RT for 10 min and then loaded into microscale thermophoresis (MST) standard or premium capillary tubes. The fluorescent signal of GFP fusion tag was detected by the blue channel of the instrument according to the manufacturer’s protocol, and the data were collected using 20% LED power and 40% MST powder at RT. Three independent experiments were performed, and the collected MST data were used to fit the dissociation constant (K _d) by using the MO. Affinity Analysis software (NanoTemper Technologies).

Integrin activation assay

Wild-type talin-H or talin-H_DM (K15A, R35A) was subcloned into a pEGFP-C1 vector. CHO A5 cells stably expressing integrin α_IIbβ₃ were transfected with empty vector, wild-type talin-H, or talin-H_DM using Lipofectamine 2000 reagent (Life Technologies). After 24 h, cells were stained with anti-α_IIbβ₃ activation-specific mAb PAC1 (1:100 dilution; Catalog# 340535, BD Biosciences) at RT for 40 min, and later incubated with Alexa Flour 647 Goat Anti-Mouse IgM (Catalog# 115-607-020, Jackson Immunology Laboratories; 1:3000 dilution) on ice for 30 min. After wash, cells were fixed and subjected to flow cytometry analysis. Values for PAC1 binding were reported as median fluorescent intensities and the data were normalized to control with empty vector only and are presented by the means ± S.E.M. The two-tailed unpaired t-test was performed to calculate the p value using GraphPad software.

Generation of cell lines and cell culture conditions

Murine talin1 and 2 double-knockout fibroblasts (talin^1/2dko)³⁸ were retrovirally transduced with pLPCX expression construct containing YPET alone, C terminally YPET-tagged talin-1 cDNA (talin WT), or YPET-tagged double-mutant talin-1 (talin DM; K15A, R35A) using the Phoenix retroviral expression system. Phoenix cells were transfected using a conventional calcium phosphate protocol and five infection cycles were performed by transferring the phoenix cell culture supernatant supplemented with 5 µg ml⁻¹ polybrene to talin^1/2dko cells^{56, 57}. Cells were FACS-sorted using a FACSAria™ IIu cell sorter (BD Biosciences, Heidelberg, Germany) to isolate cells with comparable expression levels. Cells were characterized by western blotting using mouse α-GFP (home-made), rabbit anti-talin-1(Catalog# sc-15336, Santa Cruz Biotechnology Inc.), rabbit anti-Rap1 (Catalog# sc-65, Santa Cruz Biotechnology Inc.), rabbit anti-RIAM (Catalog# ab92537, Abcam Inc.), mouse anti-GAPDH (Catalog# CB1001-500UG, Millipore), goat anti-rabbit-HRP (Catalog# 111-035-144, Jackson ImmunoResearch Laboratories), and goat anti-mouse-HRP (Catalog# 115-035-003, Jackson ImmunoResearch Laboratories Inc.) antibodies following standard protocols. Rabbit anti-talin-1, rabbit anti-Rap1, and rabbit anti-RIAM antibodies were used at a dilution of 1:1000. Mouse anti-GAPDH, goat anti-rabbit-HRP, and goat anti-mouse-HRP antibodies were used at a dilution of 1:20,000. Cells were cultured under standard conditions in DMEM GlutaMAX^TM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, 100 U ml⁻¹ penicillin, 100 µg ml⁻¹ streptomycin, and non-essential amino acids (all from Thermo Fisher Scientific).

Adhesion and spreading assays

For adhesion assays, polystyrol flat-bottom 96-well microplates (Greiner Bio-One, Frickenhausen, Germany) were coated with 10 µg ml⁻¹ laminin (Sigma) in HBSS (Thermo Fisher Scientific), 5 μg ml⁻¹ FN, 0.01 % poly-L-lysine (both Sigma), or 5 μg ml⁻¹ VN (STEMCELL Technologies, Köln, Germany) in coating buffer (20 mM Tris-HCl pH 9.0, 150 mM NaCl, 2 mM MgCl₂) overnight at 4 °C. After blocking with 3% bovine serum albumin in PBS for 30 min at room temperature, 4 × 10⁴ cells in DMEM containing 0.1 % FBS, and 25 mM HEPES were seeded per well, incubated for 1 h, and washed with PBS. Adherent cells were fixed with 4% paraformaldehyde (PFA) for 15 min and stained with 5 mg ml⁻¹ crystal violet in 2% ethanol for 30 min. After washing, remaining crystal violet was dissolved in 2% SDS and quantified by measuring absorbance at 595 nm using a microplate reader (Tecan, Männedorf, Switzerland). All experiments were performed in quadruplets. For spreading analysis, cells were plated on FN (5 μg ml⁻¹)-coated dishes and phase contrast pictures were taken using an EVOS^TM FL Auto life cell microscope (Thermo Fisher Scientific) 5, 15, 30, 60, 120, and 240 min after seeding the cells. Cell spreading area of 30 cells per group at each time point were measured using ImageJ software (US National Institutes of Health). Statistical significance was tested by two-tailed paired t-test.

Flow cytometry

Integrin surface expression was determined by FACS using a LSRFortessa^TM X-20 flow cytometer (BD Biosciences). Staining and measurement were performed in PBS supplemented with 2% FBS and 2 mM EDTA. Cells were incubated with biotinylated anti-integrin antibodies and subsequently with Cy5-labeled strepdavidin (Catalog# 016-170-084, Jackson ImmunoResearch Laboratories). Data were analyzed using FlowJo software. The following antibodies were used for flow cytometry: hamster IgM anti-integrin β1 (Catalog# 13-0291-82, eBioscience), rat IgG2a anti-integrin α6 (Catalog# 13-0495-82, eBioscience), rat IgG1 isotype control (Thermo Fisher Scientific), hamster IgG isotype control (Thermo Fisher Scientific), hamster IgG anti-integrin β3 (Catalog# 553345, BD Pharmingen), rat IgG2a anti-integrin α5 (Catalog# 557446, BD Pharmingen^TM), rat IgG1 anti-integrin αV (Catalog #104104, BioLegend), hamster IgM isotype control (BioLegend), and rat IgG2a isotype control (BioLegend). All listed antibodies were biotinylated and used at 1:200 dilution.

Immunostaining and FA analysis

Cells were cultured on FN-coated glass coverslips (Thermo Fisher Scientific) overnight or on FN-coated micropatterns (disc-shaped with 1100 µm² area; Cytoo, Grenoble, France) for 5 h and fixed for 10 min with 4% PFA. Mouse anti-paxillin with a dilution of 1:300 (Catalog# 610051, BD Transduction Laboratories, Heidelberg, Germany), goat anti-mouse-Cy3 with a dilution of 1:400 (Catalog# 115-165-146, Jackson ImmunoResearch Laboratories), and Phalloidin-Alexa Fluor 647 with a dilution of 1:100 (Catalog# A22287, Thermo Fisher Scientific) were used for immunostainings. Images were acquired using a Leica TCS SP5 X confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with ×63 numerical aperture (NA) 1.40 oil objective lenses and Leica Confocal Software (LAS AF). All pictures were processed with Photoshop (Adobe Systems, San José, CA, USA). FA area was defined by paxillin staining and quantified using ImageJ software. For quantitative analysis of talin recruitment to FAs, the total ypet signal intensity within the FA area was normalized to the total ypet fluorescence of the whole cell. Statistical significance was tested by two-tailed paired t-test.

Cell lysate preparations

Various types of cells indicated in Fig. 6a were isolated from blood or cultured cells. Cells were washed once with cold PBS, and lysed by adding buffer that contained 50 mM Tris-HCl (pH 6.8) and 1% SDS. The cell lysates were boiled for 5 min, and the concentration of total protein in each cell lysate was quantified with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Approximately 8 μg total protein for each lysate was loaded onto SDS-PAGE gels for western blotting analysis.

Data availability

Accession codes: The complete chemical shift–assignment tables of free Rap1b, bound Rap1b, and bound talin-F0 have been deposited in the Biological Magnetic Resonance Bank (BMRB) with the accession code 30353. The coordinates of Rap1b/talin-F0 complex structure have been deposited in the Protein Data Bank (PDB) with the code 6BA6. Other data are available from the corresponding authors upon reasonable request.

References

Springer, T. A. Adhesion receptors of the immune system. Nature 346, 425–434 (1990).
Article ADS CAS PubMed Google Scholar
Hynes, R. O. Integrins: bidirectional, allosteric signaling machines. Cell 110, 673–687 (2002).
Article CAS PubMed Google Scholar
Qin, J., Vinogradova, O. & Plow, E. F. Integrin bidirectional signaling: a molecular view. PLoS Biol. 2, e169 (2004).
Article PubMed PubMed Central Google Scholar
Moser, M., Legate, K. R., Zent, R. & Fassler, R. The tail of integrins, talin, and kindlins. Science 324, 895–899 (2009).
Article ADS CAS PubMed Google Scholar
Calderwood, D. A., Campbell, I. D. & Critchley, D. R. Talins and kindlins: partners in integrin-mediated adhesion. Nat. Rev. Mol. Cell Biol. 14, 503–517 (2013).
Article CAS PubMed PubMed Central Google Scholar
Arnaout, M. A. Biology and structure of leukocyte beta 2 integrins and their role in inflammation. F1000Res 5, 2433 (2016).
Goksoy, E. et al. Structural basis for the autoinhibition of talin in regulating integrin activation. Mol. Cell 31, 124–133 (2008).
Article CAS PubMed PubMed Central Google Scholar
Song, X. et al. A novel membrane-dependent on/off switch mechanism of talin FERM domain at sites of cell adhesion. Cell Res. 22, 1533–1545 (2012).
Article CAS PubMed PubMed Central Google Scholar
Goult, B. T. et al. The structure of an interdomain complex that regulates talin activity. J. Biol. Chem. 284, 15097–15106 (2009).
Article CAS PubMed PubMed Central Google Scholar
Goult, B. T. et al. Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation. J. Struct. Biol. 184, 21–32 (2013).
Article CAS PubMed PubMed Central Google Scholar
Martel, V. et al. Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides. J. Biol. Chem. 276, 21217–21227 (2001).
Article CAS PubMed Google Scholar
Yang, J. et al. Conformational activation of talin by RIAM triggers integrin-mediated cell adhesion. Nat. Commun. 5, 5880 (2014).
Article CAS PubMed PubMed Central Google Scholar
Schiemer, J. et al. Galpha13 switch region 2 relieves talin autoinhibition to activate alphaIIbbeta3 integrin. J. Biol. Chem. 291, 26598–26612 (2016).
Article CAS PubMed Google Scholar
Vinogradova, O. et al. A structural mechanism of integrin alpha(IIb)beta(3) “inside-out” activation as regulated by its cytoplasmic face. Cell 110, 587–597 (2002).
Article CAS PubMed Google Scholar
Kim, M., Carman, C. V. & Springer, T. A. Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Science 301, 1720–1725 (2003).
Article ADS CAS PubMed Google Scholar
Wegener, K. L. et al. Structural basis of integrin activation by talin. Cell 128, 171–182 (2007).
Article CAS PubMed Google Scholar
Xu, X. P. et al. Three-dimensional structures of full-length, membrane-embedded human alpha(IIb)beta(3) integrin complexes. Biophys. J. 110, 798–809 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Shattil, S. J., Kim, C. & Ginsberg, M. H. The final steps of integrin activation: the end game. Nat. Rev. Mol. Cell Biol. 11, 288–300 (2010).
Article CAS PubMed PubMed Central Google Scholar
Zeiler, M., Moser, M. & Mann, M. Copy number analysis of the murine platelet proteome spanning the complete abundance range. Mol. Cell Proteomics 13, 3435–3445 (2014).
Article CAS PubMed PubMed Central Google Scholar
Stritt, S. et al. Rap1-GTP-interacting adaptor molecule (RIAM) is dispensable for platelet integrin activation and function in mice. Blood 125, 219–222 (2015).
Article CAS PubMed PubMed Central Google Scholar
Klapproth, S. et al. Loss of the Rap1 effector RIAM results in leukocyte adhesion deficiency due to impaired beta2 integrin function in mice. Blood 126, 2704–2712 (2015).
Article CAS PubMed PubMed Central Google Scholar
Su, W. et al. Rap1 and its effector RIAM are required for lymphocyte trafficking. Blood 126, 2695–2703 (2015).
Article CAS PubMed PubMed Central Google Scholar
Chrzanowska-Wodnicka, M., Smyth, S. S., Schoenwaelder, S. M., Fischer, T. H. & White, G. C. 2nd Rap1b is required for normal platelet function and hemostasis in mice. J. Clin. Invest. 115, 680–687 (2005).
Article CAS PubMed PubMed Central Google Scholar
Nieswandt, B. et al. Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo. J. Exp. Med. 204, 3113–3118 (2007).
Article CAS PubMed PubMed Central Google Scholar
Petrich, B. G. et al. Talin is required for integrin-mediated platelet function in hemostasis and thrombosis. J. Exp. Med. 204, 3103–3111 (2007).
Article CAS PubMed PubMed Central Google Scholar
Goult, B. T. et al. Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation. EMBO J. 29, 1069–1080 (2010).
Article CAS PubMed PubMed Central Google Scholar
Plak, K., Pots, H., Van Haastert, P. J. & Kortholt, A. Direct interaction between TalinB and Rap1 is necessary for adhesion of dictyostelium cells. BMC Cell Biol. 17, 1 (2016).
Article PubMed PubMed Central Google Scholar
White, G. C. 2nd, Crawford, N. & Fischer, T. H. Cytoskeletal interactions of Rap1b in platelets. Adv. Exp. Med. Biol. 344, 187–194 (1993).
Article CAS PubMed Google Scholar
Hancock, J. F. Ras proteins: different signals from different locations. Nat. Rev. Mol. Cel. Biol. 4, 373–384 (2003).
Article CAS Google Scholar
Gloerich, M. & Bos, J. L. Regulating rap small G-proteins in time and space. Trends Cell Biol. 21, 615–623 (2011).
Article CAS PubMed Google Scholar
Chrzanowska-Wodnicka, M. Rap1 in endothelial biology. Curr Opin Hematol. 24, 248–255 (2017).
Article Google Scholar
Vaynberg, J. & Qin, J. Weak protein-protein interactions as probed by NMR spectroscopy. Trends Biotechnol. 24, 22–27 (2006).
Article CAS PubMed Google Scholar
Perera, H. D. et al. Membrane binding of the N-terminal ubiquitin-like domain of kindlin-2 is crucial for its regulation of integrin activation. Structure 19, 1664–1671 (2011).
Article CAS PubMed PubMed Central Google Scholar
Li, X. et al. Structural basis for small G protein effector interaction of Ras-related protein 1 (Rap1) and adaptor protein Krev interaction trapped 1 (KRIT1). J. Biol. Chem. 287, 22317–22327 (2012).
Article CAS PubMed PubMed Central Google Scholar
Wynne, J. P. et al. Rap1-interacting adapter molecule (RIAM) associates with the plasma membrane via a proximity detector. J. Cell Biol. 199, 317–330 (2012).
Article CAS PubMed PubMed Central Google Scholar
Das, M., Subbayya Ithychanda, S., Qin, J. & Plow, E. F. Mechanisms of talin-dependent integrin signaling and crosstalk. Biochim. Biophys. Acta 1838, 579–588 (2014).
Article CAS PubMed Google Scholar
Bouaouina, M., Lad, Y. & Calderwood, D. A. The N-terminal domains of talin cooperate with the phosphotyrosine binding-like domain to activate beta1 and beta3 integrins. J. Biol. Chem. 283, 6118–6125 (2008).
Article CAS PubMed Google Scholar
Theodosiou, M. et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. Elife 5, e10130 (2016).
Article PubMed PubMed Central Google Scholar
Pollard, T. D., Blanchoin, L. & Mullins, R. D. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu. Rev. Biophys. Biomol. Struct. 29, 545–576 (2000).
Article CAS PubMed Google Scholar
Elliott, P. R. et al. The Structure of the talin head reveals a novel extended conformation of the FERM domain. Structure 18, 1289–1299 (2010).
Article CAS PubMed PubMed Central Google Scholar
Gureasko, J. et al. Membrane-dependent signal integration by the Ras activator Son of sevenless. Nat. Struct. Mol. Biol. 15, 452–461 (2008).
Article CAS PubMed PubMed Central Google Scholar
Pizon, V., Lerosey, I., Chardin, P. & Tavitian, A. Nucleotide sequence of a human cDNA encoding a ras-related protein (rap1B). Nucleic Acids Res. 16, 7719 (1988).
Article CAS PubMed PubMed Central Google Scholar
Lilja, J. et al. SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras. Nat. Cell Biol. 19, 292–305 (2017).
Article CAS PubMed PubMed Central Google Scholar
Lagarrigue, F. et al. A RIAM/lamellipodin-talin-integrin complex forms the tip of sticky fingers that guide cell migration. Nat. Commun. 6, 8492 (2015).
Article CAS PubMed PubMed Central Google Scholar
Gavin, A. C. et al. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415, 141–147 (2002).
Article ADS CAS PubMed Google Scholar
Hein, M. Y. et al. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163, 712–723 (2015).
Article CAS PubMed Google Scholar
Williamson, M. P. Using chemical shift perturbation to characterise ligand binding. Prog. Nucl. Magn. Reson. Spectrosc. 73, 1–16 (2013).
Article CAS PubMed Google Scholar
Xu, Y., Wang, X., Yang, J., Vaynberg, J. & Qin, J. PASA--a program for automated protein NMR backbone signal assignment by pattern-filtering approach. J. Biomol. NMR 34, 41–56 (2006).
Article PubMed Google Scholar
Delaglio, F. et al. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR 6, 277–293 (1995).
Article CAS PubMed Google Scholar
Garrett, D. S., Powers, R., Gronenborn, A. M. & Clore, G. M. A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams. 1991. J. Magn. Reson. 213, 357–363 (2011).
Article ADS CAS PubMed Google Scholar
Goddard T. D. & Kneller D. G. SPARKY 3 (University of California, San Francisco). http://www.cgl.ucsf.edu/home/sparky/.
Schwieters, C. D., Kuszewski, J. J., Tjandra, N. & Clore, G. M. The Xplor-NIH NMR molecular structure determination package. J. Magn. Reson. 160, 65–73 (2003).
Article ADS CAS PubMed Google Scholar
Shen, Y., Delaglio, F., Cornilescu, G. & Bax, A. TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J. Biomol. NMR 44, 213–223 (2009).
Article CAS PubMed PubMed Central Google Scholar
Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R. & Thornton, J. M. AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J. Biomol. NMR 8, 477–486 (1996).
Article CAS PubMed Google Scholar
Moore, D. T. et al. Affinity of talin-1 for the beta3-integrin cytosolic domain is modulated by its phospholipid bilayer environment. Proc. Natl Acad. Sci. USA 109, 793–798 (2012).
Article ADS CAS PubMed Google Scholar
Austen, K. et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat. Cell Biol. 17, 1597–1606 (2015).
Article CAS PubMed PubMed Central Google Scholar
Austen, K., Kluger, C., Freikamp, A., Chrostek-Grashoff, A. & Grashoff, C. Generation and analysis of biosensors to measure mechanical forces within cells. Methods Mol. Biol. 1066, 169–184 (2013).
Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank Reinhard Fässler for providing us with talin1/2 double-knockout fibroblasts. This work was supported by NIH grants R01HL58758, R01GM62823 to J.Q., P01HL073311 to E.F.P., the Max-Planck-Society, and the SFB1123 TP A08 to M.M. We thank Jianmin Liu, Koichi Fukuda, Sujay Ithychanda, and Xi-An Mao for assistance and useful discussions.

Author information

Liang Zhu, Jun Yang and Thomas Bromberger contributed equally to this work.

Authors and Affiliations

Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH, 44195, USA
Liang Zhu, Jun Yang, Ashley Holly, Fan Lu, Huan Liu, Kevin Sun, Jamila Hirbawi, Tatiana V. Byzova, Edward F. Plow & Jun Qin
Department of Biochemistry, Case Western Reserve University, Cleveland, OH, 44106, USA
Liang Zhu, Fan Lu & Jun Qin
Max-Planck-Institute of Biochemistry, Department of Molecular Medicine, 82152, Martinsried, Germany
Thomas Bromberger, Sarah Klapproth & Markus Moser

Authors

Liang Zhu
View author publications
You can also search for this author in PubMed Google Scholar
Jun Yang
View author publications
You can also search for this author in PubMed Google Scholar
Thomas Bromberger
View author publications
You can also search for this author in PubMed Google Scholar
Ashley Holly
View author publications
You can also search for this author in PubMed Google Scholar
Fan Lu
View author publications
You can also search for this author in PubMed Google Scholar
Huan Liu
View author publications
You can also search for this author in PubMed Google Scholar
Kevin Sun
View author publications
You can also search for this author in PubMed Google Scholar
Sarah Klapproth
View author publications
You can also search for this author in PubMed Google Scholar
Jamila Hirbawi
View author publications
You can also search for this author in PubMed Google Scholar
Tatiana V. Byzova
View author publications
You can also search for this author in PubMed Google Scholar
Edward F. Plow
View author publications
You can also search for this author in PubMed Google Scholar
Markus Moser
View author publications
You can also search for this author in PubMed Google Scholar
Jun Qin
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

L.Z., M.M. and J.Q. conceived this study. L.Z. performed all biochemical/biophysical studies including NMR 2D-HSQC experiments, GST pull-down assays, ITC experiments, and MST assay. L.Z. and J.Y. performed all 3D-NMR studies and determined the solution structure of Rap1b/talin-F0 complex. T.B. and S.K. performed the functional experiments showing that the double mutations of talin resulted in defective cell adhesion and spreading, and T.B., S.K. and M.M. analyzed the data. A.H. and J.H. performed the integrin activation assay in CHO A5 cells. L.Z., F.L. and J.Y. designed and performed vesicle co-sedimentation assays. L.Z. and H.L. prepared various types of cell lysates and analyzed the expression levels of desired proteins. L.Z. and K.S. prepared ¹⁵N/¹³C-labeled Rap1 and ¹⁵N/¹³C-labeled talin-F0 for 3D-NMR studies. T.B. and E.F.P. participated in the interpretation and preparation of the manuscript. L.Z., M.M. and J.Q. wrote the manuscript with contributions from all other co-authors.

Corresponding authors

Correspondence to Markus Moser or Jun Qin.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Additional information

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Supplementary Information

Peer Review File

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Zhu, L., Yang, J., Bromberger, T. et al. Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation. Nat Commun 8, 1744 (2017). https://doi.org/10.1038/s41467-017-01822-8

Download citation

Received: 11 April 2017
Accepted: 18 October 2017
Published: 23 November 2017
DOI: https://doi.org/10.1038/s41467-017-01822-8

This article is cited by

Structure, signal transduction, activation, and inhibition of integrin αIIbβ3
- Honglei Xin
- Jiansong Huang
- Xiaofeng Shi
Thrombosis Journal (2023)
Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin
- Sawako Yamashiro
- David M. Rutkowski
- Naoki Watanabe
Nature Communications (2023)
Targeting integrin pathways: mechanisms and advances in therapy
- Xiaocong Pang
- Xu He
- Yimin Cui
Signal Transduction and Targeted Therapy (2023)
Mechanism of integrin activation by talin and its cooperation with kindlin
- Fan Lu
- Liang Zhu
- Jun Qin
Nature Communications (2022)
Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies
- Tsuyoshi Sato
- Sayaka Ishihara
- Koko Katagiri
Scientific Reports (2020)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.