Fig. 6 | Nature Communications

Fig. 6

From: Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1

Fig. 6

Mutant Atxn1 impairs the interaction of YAP/YAPdeltaC with RORα in vivo. a Immunoprecipitation analyses of mouse cerebellar cortex at P7 and P21 revealing reduced interaction between RORα with YAP/YAPdeltaC in Atxn1-KI mice (No-Dox) and the recovery of the interaction in Group I mice. b ChIP assay was performed on mouse cerebellar cortex using anti-YAP2 antibody (recognizing both full-length YAP and YAPdeltaC) or anti-YAPdeltaC antibody (recognizing only YAPdeltaC). The RORα-responsive elements in the upstream region of Pcp2, Pcp4, and Slc1a6 were amplified using specific primers. Quantitative analyses of band intensities are shown in the graphs at right. Double asterisks indicate statistical significance (p < 0.01, N = 4) in one-way ANOVA with post hoc Tukey’s HSD test. c ChIP assay was performed on primary cerebellar neurons 2 or 7 days after transfection. Expression of Myc-Atxn1-86Q decreased the amount of YAP or YAPdeltaC in the RORα transcription complex on the cis-elements of Pcp2, Pcp4, and Slc1a6, whereas mutant Atxn1 did not change the amount of RORα attached to the cis-element. Immunoprecipitation of c-Jun was performed as a negative control. Double asterisks indicate statistical significance (p < 0.01, N = 5) in one-way ANOVA with post hoc Tukey’s HSD test. d Protein levels of Myc-Atxn1-33Q and Myc-Atxn1-86Q, determined by western blot using anti-Myc antibody, in the samples shown in Fig. 6c

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