Fig. 3 | Nature Communications

Fig. 3

From: Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1

Fig. 3

Structures of RORα, YAP, and Tip60 and their possible relationship. a Scheme showing the interaction among YAP, Tip60, and RORα. GST-RORα (a.a. 424–523), used for the pull-down assay in Fig. 5d, is also shown. b Mutants generated for interaction assays. c, d In left panels, immunoprecipitation analyses with primary cerebellar neurons transiently expressing FLAG-YAP2 (full-length YAP) or FLAG-YAPdeltaC show the interaction of endogenous RORα with YAP or YAPdeltaC. In middle panels, YAP2 mutants of the first and/or second WW domain were also examined for interaction with endogenous RORα. In right panels, wild-type RORα and mutant RORα at the PPLY motif were tested for interaction with FLAG-YAP2/YAPdeltaC. The lower graphs show quantitative analyses of the relative intensities of output bands, corrected based on the corresponding input band (n = 3). Double asterisks: p < 0.01 in one-way ANOVA with post hoc Tukey’s HSD test

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