Fig. 3 | Nature Communications

Fig. 3

From: Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4

Fig. 3

Disease incidence and gene expression analysis. a, b Levels of Foc TR4 infection in selected transgenic and WT banana plants throughout the 3-year field trial. a WT and RGA2 lines, and b WT and Ced9 lines. Wet seasons (November–April) are indicated. The number of biological replicates (n) of each independent line at the start of the trial is provided in Supplementary Table 1. Data points are percentage of biological replicates infected at time of assessment. *0.01 < p < 0.05, **0.001 < p < 0.01 at trial end (Tukey's HSD test). c, d Analysis of RGA2 mRNA expression levels in transgenic and WT banana plants. c Analysis of transgene (RGA2-Nos) expression levels using primers designed to amplify a 96 bp fragment spanning the RGA2 transgene/Nos terminator junction. d Analysis of RGA2 transgene and endogenous mRNA expression levels using primers designed to amplify a 92 bp fragment of both the RGA2 transgene and RGA2 endogenous sequences. All values are normalized expression levels expressed relative to line RGA2-3. WT GN; TR4-susceptible M. acuminata ssp. malaccensis (Mal-S) and TR4-resistant M. acuminata ssp. malaccensis (Mal-R). A single biological replicate was analyzed with three technical replicates (n = 3). Data are mean ± SEM

Back to article page