Fig. 7 | Nature Communications

Fig. 7

From: Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity

Fig. 7

Immune sera contain elevated levels of oligomannose-specific antibodies with select specificity as revealed by glycan array analyses. Immune sera (n = 3) and a pre-immune control comprised of pooled pre-immune sera from the same three animals were assayed on printed glycan arrays (NCFG; version 5.3) consisting of various synthetic and naturally derived glycosides. Shown is serum IgM antibody detection at a dilution of 1:10 as measured by fluorescence (y axis). The assayed glycans are referenced by their identification numbers on the printed array (x axis). For clarity, only results (from sextuplicate replicates, with outlier signals removed) for high-mannose glycosides and partial structures thereof are shown here. Symbolic representations of the glycosides to which the immune sera bound significantly more strongly than pre-immune control are shown. Statistical significance was determined using the Holm–Sidak method (*P < 0.05). Each glycoside was analyzed individually (immune serum vs. pre-immune control), without assuming a consistent standard deviation. The chemical sequences of all arrayed glycosides are listed in Supplementary Table 1