Fig. 4 | Nature Communications

Fig. 4

From: Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity

Fig. 4

The gl precursor of the PGT128/130 families binds the lead NIT82B glycoconjugate. Binding of the predicted gl antibody was determined by flow cytometry using a biotinylated version of the lead conjugate NIT82B (designated NIT150b). Shown is the binding of the gl precursor (left) and of the affinity-matured PGT128 antibody (right), as a control, to NIT150b at 1 µM. Bound antigen was detected with PE-conjugated streptavidin and antigen binding gated on the live, antibody-expressing cell populations, which were detected with an APC-conjugated anti-human F(ab‘)2-specific antibody. The greyed histograms represent glycoconjugate binding to cells transfected with the HIV bnAb VRC01, which is specific for the CD4-binding site of HIV Env. The results are from a single experiment