Fig. 3 | Nature Communications

Fig. 3

From: Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity

Fig. 3

NAbs PGT125, 126, 128, and 130 bind avidly to NIT82B, a neoglycoconjugate presenting an Rv3-derived mimic of oligomannose. a The listed conjugates are those from Fig. 2. The conjugates (69–72 kDa) were coated as solid-phase antigen onto ELISA plate wells at 5 µg ml−1 and assayed for recognition by PGT125, 126, 128, and 130 (starting at 2 µg ml−1). All antibodies were tested as IgGs. The data represent the means from two independent experiments, with error bars omitted for clarity. GraphPad Prism (version 7.02) was used to determined EC50 values (0.1–5 nM). b To assess the importance of valency for antibody binding, conjugate NIT82B was coated onto ELISA plates (5 µg ml−1) and antibody binding assessed in the presence of the unconjugated 3-aminopropyl glycosides NIT68A (presented on NIT82A), NIT68B (presented on NIT82B) or NIT70A (presented on NIT83A), or the NIT82B glycoconjugate as a comparator (dashed line). The results, obtained from one experiment, show that the antibodies are inhibited best by the NIT82B conjugate