Fig. 1 | Nature Communications

Fig. 1

From: Characterizing a thermostable Cas9 for bacterial genome editing and silencing

Fig. 1

The Geobacillus thermodenitrificans T12 type-IIC CRISPR-Cas locus encoding ThermoCas9. a Schematic representation of the genomic locus encoding ThermoCas9. The domain architecture of ThermoCas9 based on sequence comparison, with predicted active sites residues highlighted in magenta. A homology model of ThermoCas9 generated using Phyre 257 is shown, with different colors for the domains. b Phylogenetic tree of Cas9 orthologues highly identical to ThermoCas9. Evolutionary analysis was conducted in MEGA758. c SDS-PAGE of ThermoCas9 after purification by metal-affinity chromatography and gel filtration. The migration of the obtained single band is consistent with the theoretical molecular weight of 126 kD of the apo-ThermoCas9

Back to article page