Fig. 2 | Nature Communications

Fig. 2

From: A thermostable Cas9 with increased lifetime in human plasma

Fig. 2

PAM identification and engineering of GeoCas9. a WebLogo for sequences found at the 3′ end of protospacer targets identified with CRISPRTarget for Geobacillus stearothermophilus (left) and Geobacillus LC300 (right). b Cleavage assays conducted with the two homologs of GeoCas9. Substrates with various PAM sequences were 32P-labeled and mean ± S.D. is shown. c Mapping of mutated residues (orange spheres) between G. st. and G. LC300 onto the homology model of GeoCas9 showing high density in the PAM-interacting domain near the PAM region of the target DNA. d Alignment of the Cas9 proteins from G. st. and G. LC300 with the domain boundaries shown above. Solid colors represent identical residues and gray lines indicate residues that are mutated between the two Cas9 homologs

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