Fig. 1 | Nature Communications

Fig. 1

From: A thermostable Cas9 with increased lifetime in human plasma

Fig. 1

GeoCas9 is a thermostable Cas9 homolog. a Phylogeny of Cas9 proteins used for genome editing with their length (amino acids) and the maximum temperatures that supports growth of the host indicated to the right22. b Homology model of GeoCas9 generated using Phyre 247 with the DNA from PDB 5CZZ docked in. c Schematic illustration of the domains of Spy Cas9 (blue) and GeoCas9 (orange) with active site residues indicated below with asterisks. d Representative traces for differential scanning calorimetry (DSC) of GeoCas9 and SpyCas9. e Denaturation temperature of various Cas9 proteins as measured by DSC, mean ± S.D. is shown. Nme Neisseria meningitides, Geo Geobacillus stearothermophilus, Geo LC300 Geobacillus LC300, Spy Streptococcus pyogenes, Sau Streptococcus aureus, Fno Francisella novicida, Sth (3) Streptococcus thermophilus CRISPR III, T d denaturation temperature

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