Fig. 2 | Nature Communications

Fig. 2

From: OCT4 impedes cell fate redirection by the melanocyte lineage master regulator MITF in mouse ESCs

Fig. 2

MITF efficiently transdifferentiates MEFs. a Bright field images of Mitf knock-in MEFs showing morphological changes at 12 days after Mitf induction by Dox supplementation and of untreated Mitf knock-in MEFs and primary mouse melanocytes. This was one of n = 3 experiments. b MITF and TYRP1 protein levels in Mitf knock-in MEFs at days 6 and 12 post Dox induction. This was one of n = 3 experiments. c Immunostaining of MITF (green) and TYRP1 (purple) in MEFs at day 6 post Dox induction with endogenous expression of mCherry (red). This was one of n = 3 experiments. d Hierarchical clustering of genes differentially expressed in MEFs before and after induction of Mitf expression (5864 genes) using Spearman correlation as a distance metric, Ward’s linkage, and per-row standardization (z score). e Spearman’s correlation was calculated over all exons that show differential ( > 4 fold change) expression between MEFs and melanocyte samples (45,155 exons)

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