Fig. 4 | Nature Communications

Fig. 4

From: Stress-induced plasticity of dynamic collagen networks

Fig. 4

The micromechanics of collagen ECM in the vicinity of cell-induced collagen bundles after traction forces are released. a The confocal reflection image and directional compliance given by five probe particles around a collagen bundle in a typical experiment. The compliance is scaled linearly into real space such that an isotropic response of 0.5 Pa−1 would be plotted as a ring with the size of the bottom right circle. Magenta dots: experimentally measured directional compliance. Red circles: the compliance ellipse, i.e., the elliptical fit to the magenta dots. White dashed lines: outlines of MDA-MB-231 cells after Cytocytochalasin-D treatment. Green line: the location of collagen bundle. Scale bar: 50 μm. b The aspect ratios of the compliance ellipses at varying particle-to-bundle distances d. Symbols of different colors correspond to results measured around different bundles. We divide all the data into three groups d < 25 μm, 25 ≤ d < 50 μm, and 50 ≤ d < 75 μm. Error bars represent the mean and standard deviations of each group. ANOVA analysis shows that the aspect ratios close to the collagen bundles (d < 25 μm) are significantly higher than the values further away

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