Fig. 1 | Nature Communications

Fig. 1

From: Stress-induced plasticity of dynamic collagen networks

Fig. 1

Cell traction forces irreversibly induce the formation of collagen bundles. a Reconstructed streamlines showing the spatial-temporal profile of the cell-induced matrix deformation. The deformation field from frame to frame is calculated via reflectance particle image velocimetry22. Color code (blue to red) is linearly proportional to maturation time 0−30 min). b Confocal reflection image of the collagen matrix showing a collagen bundle (arrow) between two MDA-MB-231 cells. c Collagen bundles simultaneously form between multiple cell pairs. Red: GFP-labeled MDA-MB-231 cells. Green: reflectance image of collagen fibers. d The relative reflectance intensity ΔF/F of collagen bundles compared to the background. e ΔF/F of collagen bundles after disrupting the cell traction forces by Cytochalasin-D treatment. In d, e, ~30 cell pairs are sampled for each maturation time. The thick black lines, box edges and whiskers represent the median, first/third quartiles, and lower/upper 5% values, respectively. ANOVA and Fisher’s least significant difference procedure is used to evaluate the difference of ΔF/F corresponding to different maturation times. *p < 0.05, **p < 0.01. Differences between non-labeled pairs are not significant. f Fraction of permanent collagen bundles F perm as a function of maturation time. Scale bar: 20 μm

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