Fig. 2 | Nature Communications

Fig. 2

From: Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking

Fig. 2

Parallax-DIC microscope and 5D-SPT instrumental set-up. a Schematic illustration of the optical path in parallax-DIC microscope. High-precision objective scanner was not drawn in the scheme. b Model of the insert mounted with the custom-cut wedge prism. c Model of the slot. d Photograph of the wedge prism set installed on the 3D-printed insert housing in the 3D-printed slot. The position of the insert and wedge prism were optimized and fixed by four #4/40 × 1/8 socket set screws and two #4/40 × 1/2 hex socket head cap screws to split the light in half at the back focal plane of the objective. e Photograph of the 5D-SPT instrumental set-up. The optimized wedge prism set was insert to the light path (as indicated in the red dashed box) to enable the parallax-DIC mode and the objective scanner (as shown in the blue dashed box) was use to deploy the automated 5D-SPT

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