Fig. 2 | Nature Communications

Fig. 2

From: Relationships between structure, in vivo function and long-range axonal target of cortical pyramidal tract neurons

Fig. 2

Identifying subcortical targets of in vivo recorded PTs. a Cell-attached recording and biocytin labeling of exemplary neuron in L5, after retrograde tracer injections into the SC (FG), Pons (CTB-594), and POm (CTB-647). Slices were stained with Alexa-405 and Alexa-488 to reveal FG-positive and biocytin-labeled neurons (white arrow), respectively. Right panel: 3D dendrite reconstruction of the exemplary PT. b Top panels: raster plots and PSTHs of spiking activity before (ongoing), during, and after whisker stimulation (red dashed lines) for three example neurons. Bottom panels: 3D dendrite reconstructions and identification of the respective subcortical targets of the three example PTs

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