Fig. 8 | Nature Communications

Fig. 8

From: Survival of pancreatic cancer cells lacking KRAS function

Fig. 8

Multiple dysregulated cellular processes in KRAS deficient cells. a Unsupervised hierarchical clustering dendrograms of 8988T and A13 clones indicated clean segregation between KRAS intact and deficient (KO) cells within each cell line. b Network representation of overlapping enriched GSEA/MSigDB gene sets in the A13 knockout signature (p < 0.05, FDR < 0.25). Each circle represents a gene set with circle size corresponding to gene set size and intensity corresponding to enrichment significance. Red is upregulated and blue is downregulated. Each line corresponds to minimum 50% mutual overlap with line thickness corresponding to degree of overlap. Cellular processes associated with related gene sets are listed. c Network representation of overlapping enriched GSEA/MSigDB gene sets in the 8988T knockout signature (p < 0.005, FDR < 0.1). d Heat map of a 32-gene combined knockout signature generated through ICA analysis of A13 and 8988T gene expression data sets. Gene names are listed in rows. Row normalized gene expression values are shown where red designates relative upregulation and blue designates relative downregulation. e Kaplan–Meier plots of survival of human patients from the TCGA PDAC cohort whose tumors most correlated (top quintile) and least correlated (bottom quintile) with the combined knockout signature in d (UP genes only). Log-rank (Mantel–Cox) p value is shown. f Kaplan–Meier plots of survival of human patients from the ICGC cohort whose tumors most correlated (top quintile) and least correlated (remaining tumors) with the combined knockout signature in d (UP genes only). Log-rank (Mantel–Cox) p value is shown

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