Fig. 5 | Nature Communications

Fig. 5

From: Survival of pancreatic cancer cells lacking KRAS function

Fig. 5

MAPK blockade following PI3K inhibition in KRAS deficient cells. a Western blot showed no change in pERK1/2 levels in KRAS intact cells at designated times (minutes for A13, hours for 8988T) following GDC-0941 treatment. HSP90 is loading control. b Western blot demonstrated a transient decrease in pERK1/2 levels in KRAS deficient cells at designated times (minutes for A13, hours for 8988T) following GDC-0941 treatment. c Western blot showed a transient decrease in phosphorylation of the MAPK pathway regulators CRAF and MEK1/2 following GDC-0941 treatment in KRAS deficient cells. d Western blot of RAS-GTP levels in KRAS intact (E6) and deficient (H36) clones following 1-h treatment with GDC-0941 showed a specific decline in deficient cells. e Overexpression of constitutively active MEK (MEK-DD) or oncogenic KRAS-G12V, but not KRAS-WT or GFP, blocked pERK1/2 inhibition by a 4-h treatment with GDC-0941. f MEK-DD and KRAS-G12V-transduced cells from e showed decreased sensitivity to PI3K inhibition compared to control GFP- and KRAS-WT-transduced cells