Fig. 2 | Nature Communications

Fig. 2

From: Survival of pancreatic cancer cells lacking KRAS function

Fig. 2

KRAS is dispensable in a subset of PDAC cell lines. a Western blot confirmed loss of KRAS protein in knockout clones derived from PANC-1 (P2 complete, P3 partial), KP-4 (P1, P2, P3, P4), and MM1402 (H1, H2) cell lines compared to intact clones (PANC-1-E1,E2; KP-4-E1,E2; MM1402-E1,E2,E3). HSP90 is loading control. b KRAS deficient clones (purple) exhibited altered cell morphology compared to intact cells (gray). Specific differences include increased cell size, cytoplasmic translucency, and smooth edges. Scale bar is 100 µm. c KRAS deficient clones showed diminished proliferation in vitro. Average cell viability (normalized to day 0) ± s.e.m. for each clone is plotted. PANC-1 knockout clone (P2) and partial knockout clone (P3) exhibited a dose-dependent effect of KRAS expression on proliferation compared to intact clones (E1, E2). d PANC-1, KP-4, and MM1402 KRAS deficient clones showed diminished soft agar colony formation. PANC-1 cells displayed a dose-dependent effect of KRAS expression on anchorage-independent growth. Scale bar is 500 µm